Method for improving photosynthetic hydrogen production efficiency of algae

A technology of algae and high efficiency, which is applied in the field of improving the photosynthetic hydrogen production efficiency of algae, and can solve the problems of Chlamydomonas photosynthetic hydrogen production efficiency extension duration, high cost, and Chlamydomonas photosynthetic hydrogen production cannot be industrialized, and achieve photosynthetic hydrogen production efficiency Improved effect

Inactive Publication Date: 2012-11-14
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although great progress has been made in the research of Chlamydomonas photosynthetic hydrogen production, it is still impossible to maintain high efficiency and prolong the duration of Chlamydomonas photosynthetic hydrogen production. change

Method used

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  • Method for improving photosynthetic hydrogen production efficiency of algae
  • Method for improving photosynthetic hydrogen production efficiency of algae
  • Method for improving photosynthetic hydrogen production efficiency of algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0177] Embodiment 1, identification of transgenic Chlamydomonas

[0178] PCR method was used to detect whether the exogenous gene was integrated into Chlamydomonas chloroplast genome, and whether the exogenous gene was expressed after heat shock induction. figure 2 B shows that the expression of exogenous genes POX and CAT cannot be detected using the DNA of wild-type Chlamydomonas cc503 as a template. However, using the DNA of the transgenic Chlamydomonas ccHPC as a template, very specific bands of exogenous genes POX and CAT were detected.

[0179] The results showed that exogenous genes POX and CAT had been integrated into the genome of the transgenic Chlamydomonas.

Embodiment 2

[0180] Embodiment 2, the expression of exogenous gene of transgenic Chlamydomonas

[0181] At the beginning, the inventors adopted a culture method of heat shock once a day, and found that the expression level of the exogenous gene was relatively low.

[0182] Therefore, the induction method of heat shock at 40°C every 6 hours was adopted. For the specific procedure, see figure 2 A, The expression of exogenous genes POX and CAT has been at a high level throughout the culture period.

Embodiment 3

[0183] Embodiment 3, the detection of growth rate

[0184] Since the transgenic Chlamydomonas introduced two exogenous genes, in order to detect whether the expression of the exogenous genes affects the growth rate of Chlamydomonas itself, the inventors tested the growth rate of Chlamydomonas at the same initial concentration. image 3 (Experimental conditions are: continuous low light (light intensity is 75umol photons m -2 the s -1 ) culture and heat shock every 6 hours) showed that the growth rate of the transgenic Chlamydomonas ccHPC was only slightly lower than that of the wild-type Chlamydomonas cc503, and there was no significant difference between the two. This indicated that although the expression of the exogenous gene changed the metabolic response of Chlamydomonas itself, it did not cause a significant inhibitory effect.

[0185] In the practice of metabolic engineering transformation, the growth of many transgenic species is lower than that of wild-type species,...

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Abstract

The invention relates to a method for improving photosynthetic hydrogen production efficiency of algae. The method expresses pyruvate oxidase and catalase in hydrogen production algae with no influence on normal metabolism and growth of the hydrogen production algae, so that oxygen concentration in the hydrogen production algae can be decreased effectively; photorespiration function is reduced; and photosynthetic hydrogen production efficiency of the hydrogen production algae is increased greatly. The method provides novel approaches for improving photosynthetic hydrogen production efficiency, and provides novel ideas for bioenergy research and industrialization.

Description

technical field [0001] The invention belongs to the field of biotechnology; more specifically, the invention relates to a method for improving the photosynthetic hydrogen production efficiency of algae. Background technique [0002] A considerable part of algae can produce hydrogen through photosynthesis, so algae are studied as a kind of organisms capable of producing hydrogen. When hydrogen-producing algae are cultured under anaerobic conditions for a period of time, the oxygen produced by their photosynthesis is consumed by their own respiration, and the low-oxygen environment induces the expression of hydrogen-producing enzymes in the chloroplast stroma, which receive electron transport chain The electrons reduce the hydrogen ions produced by the photolysis of water to generate hydrogen gas. Usually, because the rate of oxygen production by photosynthetic hydrolysis is higher than the rate of oxygen consumption by respiration, the oxygen concentration in cells is high, ...

Claims

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Application Information

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IPC IPC(8): C12N15/79C12N1/13C12P3/00C12R1/89
Inventor 徐福桥朱新广孙宇静
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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