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Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof

A technology of microfluidic chips and pesticide residues, applied in chemical instruments and methods, measuring devices, laboratory containers, etc., can solve problems such as no substantive breakthroughs, achieve good application prospects, short detection time, The effect of small dosage

Inactive Publication Date: 2012-11-21
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, using a wafer-shaped chip, preparing microstructures and microchannels on its surface for analysis, relying on centrifugal force to drive sample microfluidics, and completing the detection of several samples at the same time, the centrifugal microfluidic chip analysis technology is currently used in the rapid detection of pesticide residues. There has been no substantial breakthrough in the field of application

Method used

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  • Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof

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Experimental program
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Embodiment 1

[0035] The microstructure and microchannel pattern of the centrifugal microfluidic chip for detecting pesticide residues were designed and drawn by computer-aided design software. The microstructures and microchannels were prepared on the three-layer polycarbonate (PC) chip by the CNC micromachining system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the surface of the chip were wiped with ethanol. On the double-layer mechanoadhesive film, the desired microstructures and microchannels were prepared with a plotter. Place 40mg of shredded vegetable samples in the extraction pool, align, bond, and pressure-seal 3-layer PC chips and 2-layer adhesive films to make a centrifugal microfluidic chip for detecting pesticide residues. Add 120 μL of phosphate buffer (pH=7.71) to the extraction pool, add 60 μL of acetylcholinesterase solution (40 U / mL) to the inhibition reaction pool, and add 20...

Embodiment 2

[0037]The microstructure and microchannel pattern of the centrifugal microfluidic chip for detecting pesticide residues were designed and drawn by computer-aided design software. The microstructures and microchannels were prepared on the three-layer polymethyl methacrylate (PMMA) chip by the CNC micromachining system. The chips of each layer were cleaned with tap water and distilled water respectively, and the remaining fingerprints, oil stains and other stains on the surface of the chip were wiped with ethanol. On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. Weigh 40 mg of soil samples and place them in the extraction tank, align, bond, and pressure seal the 3-layer PMMA chip and 2-layer double-sided adhesive film to make a centrifugal microfluidic chip. Add 120 μL of phosphate buffer (pH=7.71) to the extraction pool, add 60 μL of acetylcholinesterase solution (40 U / mL) to the inhibition reaction pool, a...

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Abstract

The invention relates to a centrifugal micro-fluidic chip for detecting pesticide residue and a preparation method thereof. The centrifugal micro-fluidic chip for detecting the pesticide residue is a disk type chip with a micro structure and a micro channel and consists of multiple layers of chips; the mixing, extracting, reacting, separating and developing process of a sample to be measured and a reagent is realized under the drive of a centrifugal force generated by rotating a centrifugal machine; and finally, the content of the pesticide residue in the sample is quantitatively detected by using an ultraviolet visible light spectrophotometer. The centrifugal micro-fluidic chip for detecting the pesticide residue can be used for directly sampling without pretreating the sample; the consumption of the sample and the reagent is low; a plurality of samples can be simultaneously treated and detected in parallel, and thus the integration, the micromation and the automation of the pesticide residue detection are realized; and the centrifugal micro-fluidic chip has the characteristics of economy, quickness and portability and provides a brand-new analysis technology for quickly detecting the pesticide residue of water bodies, foods and soil.

Description

technical field [0001] The invention relates to a centrifugal microfluidic chip for detecting pesticide residues and a preparation method thereof. Driven by the centrifugal force generated by the rotation, the process of mixing, extraction, reaction, separation and color development between the sample to be tested and each reaction reagent is realized on the chip, and finally the pesticide residue content in the sample is quantitatively detected by a UV-visible spectrophotometer. The main application In health supervision, food safety, environmental testing and other related fields. Background technique [0002] Organic pesticides, as a class of high-efficiency and broad-spectrum insecticides, are an important means to prevent and control diseases and insect pests and improve crop quality and yield. The lack of knowledge and one-sided pursuit of production have resulted in the existence of pesticide residues in soil, water, and food to varying degrees, resulting in many pot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/01G01N21/33
Inventor 王晓东沙俊聂富强
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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