Preparation method of controlled release antibacterial film

A technology of lysozyme and film-forming matrix is ​​applied in the field of preparation of controlled-release antibacterial films, which can solve the problems of slow release rate and fast release rate, and achieve the effects of reducing damage, improving efficiency, and increasing the upper limit of addition amount.

Active Publication Date: 2012-11-21
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The antibacterial agent added directly will interact with the microorganisms after being released from the membrane network to the outside world, and the release rate is relatively fast; the antibacterial agent added after embedding needs to be released from the nanoparticles into the membrane network before that, and the release rate is relatively slow

Method used

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  • Preparation method of controlled release antibacterial film
  • Preparation method of controlled release antibacterial film
  • Preparation method of controlled release antibacterial film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Accurately weigh 0.5g of zein and 0.1g of thymol, dissolve in 20mL of 80% ethanol-water solution; dissolve 0.5g of sodium caseinate in 50ml of deionized water, quickly pour it into the stirring alcohol solution, At a temperature of 50 ° C, a vacuum of 0.1 Mpa was rotated for 20 min, centrifuged at 8000 rpm for 5 min and freeze-dried. Dissolve 1g of gelatin in 20ml of water, stir at 60°C for 10min, add 0.2g of freeze-dried nanoparticle powder, 40mg of lysozyme and 0.24g of glycerin, pour the film after stirring, and dry at 40% RH and 25°C for 24 hours. A mixed film is obtained.

[0041] The physicochemical performance parameter of the nanoparticle powder that embodiment obtains is as follows:

[0042] The particle size is 182.8nm, the PDI value is 0.22, the potential is -39.2mv, the yield is 79.4%, and the encapsulation efficiency is 84.6%.

[0043] The nano particle powder of the invention can be used as a food protein nano antibacterial embedding and delivery carrier...

Embodiment 2

[0047] Accurately weigh 0.5g of zein and 0.1g of thymol, dissolve in 20mL of 70% ethanol-water solution; dissolve 0.5g of sodium caseinate in 50ml of deionized water, quickly pour it into the stirring alcoholic solution, At a temperature of 60 ° C, a vacuum of 0.1 Mpa was rotated for 10 min, centrifuged at 1000 rpm for 10 min and freeze-dried. Dissolve 1g of sodium caseinate in 20ml of water, add 0.2g of freeze-dried nanoparticle powder, 40mg of lysozyme and 40u of TG enzyme, stir at 45°C for 4 hours, add 0.48g of glycerin, pour the membrane after stirring, and put it in RH40 %, and dried at 25°C for 24 hours to obtain a cross-linked mixed film.

[0048] The result of its release is as figure 2 shown.

[0049] From figure 2 It can be seen that the release rate of directly loaded lysozyme is still faster, and the release rate of loaded thymol after embedding is still slow. However, the rate of release has been greatly reduced compared to that of uncrosslinked membranes. ...

Embodiment 3

[0053]Accurately weigh 0.5g zein, dissolve it in 20mL 90% ethanol-water solution; , rotary evaporated at a vacuum of 0.1 Mpa for 20 min, centrifuged at 8000 rpm for 5 min and freeze-dried. Take 0.2 g of freeze-dried nanoparticle powder and 1 g of sodium caseinate, dissolve them in 30 ml of water, add 0.36 g of glycerin, pour the film after stirring, dry at RH40%, 25 ° C for 24 hours to obtain the sample film.

[0054] The physical and chemical performance parameters of nanoparticle powder are as follows:

[0055] The particle size is 184.2nm, the PDI is 0.22, the potential is -37.2mv and the yield is 79.6%.

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Abstract

The invention discloses a preparation method of a controlled release antibacterial film. The method includes the following steps of: (1) dissolving zein and a hydrophobic antibacterial agent into an alcoholic solution; (2) preparing a sodium caseinate aqueous solution; (3) adding the sodium caseinate aqueous solution into the solution obtained in step (1) under stirring; (4) subjecting the solution obtained in step (3) to rotary evaporation and centrifugation, then freeze drying the supernatant, thus obtaining nanoparticle powder; (5) dissolving a film-forming matrix in water, adding the nanoparticle powder and lysozyme to form a mixed solution; and (6) adding glycerin, and conducting film pouring, thus obtaining the film product. The method provided in the invention can simultaneously reduce initial microbial activity and inhibit microbial growth for a long term, and has the advantages of low instrument requirement, and controllable bacterium inhibiting intensity, thus having broad application prospects in food and medical products.

Description

technical field [0001] The invention relates to a method for preparing a controlled-release antibacterial film. The raw materials are all food materials, and belong to the technical field of deep processing of agricultural by-products and food industry. Background technique [0002] The growth of pathogenic microorganisms in the process of food processing and storage is an important factor leading to food spoilage, so ensuring food safety and extending food shelf life are key scientific and technological issues in the food field that need to be solved urgently. Adding natural antibacterial substances to food instead of chemically synthesized preservatives has become a trend. Thymol, lysozyme, and nisin are all natural antibacterial substances that exist in nature, but their application in food is limited due to factors such as pungent odor, low solubility, and short effective antibacterial time. Therefore such antibacterial agents need to be loaded into the carrier. The an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08J3/24C08L89/00C08L5/08C08K5/053C08K5/13
Inventor 尹寿伟黎亢抗杨晓泉
Owner SOUTH CHINA UNIV OF TECH
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