Method for separating and preparing high-purity acylated delphinidin derivatives from eggplant peel
A technology of delphinidin and eggplant skin, which is applied in the field of preparation of delphinidin derivatives, can solve the problems of high price and few anthocyanin standard types, and achieve the effect of simple operation and easy implementation
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Embodiment 1
[0024] 5 kg of fresh eggplants, cleaned, sorted, separated from the skin and flesh to obtain 750 g of eggplant skins, chopped; mixed with 1.5 L, 0.01% methanolic hydrochloric acid solution (v / v), extracted at room temperature for 2 hours; after extraction, passed through a Buchner funnel Suction filtration, and the filtrate was rotatively evaporated at 43°C to remove methanol to obtain 50 mL of anthocyanin extract; add 100 mL of ethyl acetate to extract, extract three times, each time the amount was 100 mL, and the obtained extract was concentrated by rotary evaporation at 42°C to obtain anthocyanin Extract: dissolving the extract with 10-15mL of 0.01% (v / v) hydrochloric acid aqueous solution to obtain anthocyanin loading solution;
[0025] Rinse XAD-7HP macroporous resin column with 0.01% (v / v) hydrochloric acid aqueous solution Carry out pre-acidification, inject anthocyanin sample solution at a flow rate of 0.5BV / h, after the adsorption is completed, rinse the resin with d...
Embodiment 2
[0027] 10kg of fresh eggplants, cleaned, sorted, separated from the skin and flesh to obtain 1500g of eggplant skins, chopped; mixed with 3L, 0.01% methanolic hydrochloric acid solution (v / v), leached at room temperature for 2h; Filter, and the filtrate was evaporated at 43°C to remove methanol, and 150 mL of anthocyanin extract was obtained; 300 mL of ethyl acetate was added for extraction, and the extraction was performed three times, each time with an amount of 300 mL, and the obtained extract was concentrated by rotary evaporation at 42°C to obtain anthocyanin extract cream; the extract is dissolved with 0.01% (v / v) hydrochloric acid aqueous solution of 30-50mL to obtain anthocyanin loading sample liquid; wash the XAD-7HP macroporous resin column (ratio Surface area is 500m 2 / g; the average pore diameter is The average particle size is 560μm; the dipole moment is 1.8; the uniformity coefficient D90 / D40 is 1.7; ) was pre-acidified, and the anthocyanin sample solution was...
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