Biological sensitive detecting method for heavy metal cadmium in seawater

A heavy metal, cadmium pollution technology, applied in recombinant DNA technology, DNA/RNA fragment, fluorescence/phosphorescence, etc., can solve the problem of no technical report

Inactive Publication Date: 2014-05-14
TIANJIN NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

In addition, the current domestic water heavy metal biomarker technology is limited to the measurement of stress protein content and activity
There is no formal technical report on the use of stress protein gene expression process as a monitoring method for heavy metal cadmium pollution

Method used

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  • Biological sensitive detecting method for heavy metal cadmium in seawater
  • Biological sensitive detecting method for heavy metal cadmium in seawater
  • Biological sensitive detecting method for heavy metal cadmium in seawater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Cadmium ions (Cd 2+ ) Determination of the expression of HSP70 gene under the safe concentration of green clam

[0078] 1. Safe Concentration Experiment of Heavy Metal Cadmium Stress

[0079] The safe concentration refers to the highest concentration of toxic substances that can survive, grow and reproduce normally under the continuous action of pollutants. The currently widely used formula for calculating the safe concentration is:

[0080] Safe concentration = half-lethal concentration × application coefficient, namely SC = LC50 × f (Zhou Yongxin et al., 1983).

[0081] The selection of the application factor f is based on the toxicity of the poison, whether the physical and chemical properties are stable, whether it can accumulate in the organism, etc., and is generally selected in the range of 0.01~0.1. Under this concentration, the experimental organisms can survive normally under the stress of accepting toxic substances to the maximum, without affecting the vi...

Embodiment 2

[0144] Comparative test: The detection of metal ions in conventional water bodies is often done by spectroscopic techniques, such as ICP inductively coupled plasma emission spectroscopy. Although these analytical techniques can carry out more accurate quantitative determination of element content, there are disadvantages such as large initial investment and high operating cost. More importantly, these methods only measure the content of the total elements in the sample, and cannot measure indicators such as their valence state, existing form, and damage to organisms. For metal elements, the form that causes harm to organisms is often dissolved in the water body, and conventional quantitative analysis methods are difficult to analyze this data, which is not conducive to the degree of impact of water pollution on organisms. Effective analysis and evaluation. In addition, if the content of some heavy metal elements in the water body is low, the sample needs to be concentrated or...

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Abstract

The invention discloses an efficient and simple biological sensitive detecting method for heavy metal cadmium in seawater. Particularly, the method includes respectively placing Chinese Venuses in seawater containing heavy metal cadmium (two-valence Cd) and no cadmium (two-valence Cd), dissecting for 96 hours to obtain liver tissue of the Chinese Venuses, extracting total RNA (ribonucleic acid) and transcribing into cDNA (deoxyribonucleic acid), and measuring difference of expression quantity of HSP70 (hot shock protein 70) genes of the Chinese Venuses in an experiment group and that of the Chinese Venuses in a control group by a fluorescence quantitative PCR (polymerase chain reaction) method. The larger the content of the heavy metal cadmium (two-valence Cd) in water, the larger the relative expression quantity of HSP genes, and low-concentration cadmium (two-valence Cd) in water, which is hard to detect by analytic instruments, can be observed. The method can be used as a normal biological technique for detecting pollution level of the heavy metal cadmium in seawater and difference among different water areas, has the advantages of high analytic sensitivity, high comparison and the like and can be used to judge harmful risks of the heavy metal cadmium (two-valence Cd) to water areas and biological bodies, and irreparable loss caused by severe situation is avoided.

Description

[0001] This application was supported by the Tianjin Municipal Science and Technology Commission Application Fundamentals and Frontier Technology Key Project (12JCZDJC22800) and the Tianjin Science and Technology Commission Application Foundation and Frontier Technology Key Project (09JCZDJC19300). technical field [0002] The invention belongs to the field of biological probe monitoring of marine pollution, relates to aquatic animal molecular biology technology, and specifically relates to a novel, efficient and sensitive biological detection method for heavy metal cadmium in seawater. Background technique [0003] In recent years, with the rapid development of my country's mining, smelting, ceramics, electroplating, pigments, and electronics industries, harmful heavy metals such as cadmium, arsenic, and mercury have been discharged into nature in large quantities in the form of industrial "three wastes", which not only pollute the soil environment such as cultivated land , w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11G01N21/64
Inventor 潘宝平张丽岩罗凯娅
Owner TIANJIN NORMAL UNIVERSITY
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