Human cytomegalo virus immunogen fusion protein as well as preparation method and usage thereof

Abstract

The invention discloses a human cytomegalovirus immunogen fusion protein, which includes flagellin, and human cytomegalovirus envelope glycoprotein or its polypeptide fragment; the invention discloses a DNA molecule encoding the aforementioned fusion protein, and a DNA molecule comprising the DNA Molecular recombinant plasmid and recombinant expression vector; the invention discloses the preparation method and application of the fusion protein; the invention also discloses a human cytomegalovirus vaccine. The fusion protein of the invention has strong immunogenicity, can stimulate the body to produce a large amount of anti-cytomegalovirus antibodies, is used for preparing vaccines to prevent diseases caused by cytomegalovirus infection, and has good clinical application prospects.

Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to a human cytomegalovirus immunogen fusion protein and a preparation method thereof. Background technique

[0002] Human cytomegalovirus (HCMV) belongs to the double-stranded DNA virus of the β-herpesvirinae subfamily, and is the most common opportunistic infection virus, and its infection is species-specific. The infection rate of HCMV showed obvious differences in different places and ages. In developed countries, the positive rate of HCMV antibodies is 50% to 80%. In developing countries, 80% of children are infected with HCMV before the age of 3, and the HCMV infection rate can even reach 100% in adults. The infection rate among Chinese population is as high as 86%-96%. Most healthy people have subclinical infection or latent infection without obvious clinical symptoms, and some patients may have symptoms similar to infectious mononucleosis. However, HCMV infection is inde...

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