Application of polyphenol compound to preparation of pharmaceutical for treating senile dementia

A technology of polyphenolic compounds and senile dementia, which is applied in the field of application of polyphenolic compounds in the preparation of drugs for the treatment of senile dementia, and can solve problems such as senile dementia

Active Publication Date: 2013-01-02
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hypothesis holds that the excessive production, accumulation and deposition of Aβ in the brain will l

Method used

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  • Application of polyphenol compound to preparation of pharmaceutical for treating senile dementia
  • Application of polyphenol compound to preparation of pharmaceutical for treating senile dementia
  • Application of polyphenol compound to preparation of pharmaceutical for treating senile dementia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Preparation and structural identification of compounds

[0020] 1. Preparation of compounds

[0021] Take 5.5kg of dried raisin powder, take 500g each time, add 4L of 60% ethanol, heat and reflux, filter after 2.5h, and collect the filtrate; the filter residue is heated and refluxed with 60% ethanol for 3 times, filtered, and the filtrate is collected; The second filtrate was evaporated to dryness under reduced pressure to obtain 600g of grape vine ethanol extract (V).

[0022] Wash the HP20 type macroporous resin alternately with ethanol and distilled water until the washing solution is clear, then wash with distilled water until there is no ethanol, pack it into a column, and set aside; add 6L of distilled water to 550g of the ethanol extract of grape vine, stir to dissolve the alcohol extract of grape berry , and then centrifuged, the supernatant is the grapevine water solution; then, the precipitate part is then dissolved with 20% ethanol, and then ce...

Embodiment 2

[0026] Embodiment 2: anti-senile dementia effect of compound

[0027] 1. Miyabe orcorol C reduces the production of Aβ by weakening its amyloid cleavage of APP by inhibiting the activity of β-secretase (BACE-1).

[0028] 1.1 Materials and Instruments

[0029] Experimental cells: human β-amyloid precursor protein APP 695 Stably transfected mouse neuroblastoma cell line (N 2 a695).

[0030] Solution preparation:

[0031] N 2 a695 cell culture medium: 45% DMEM+50% Opti-MEM+5% fetal bovine serum+1% double antibody stock solution+400μg / mLG418.

[0032] TNEN cell lysate: 10mM Tris (pH7.5), 150mM NaCl, 1mM EDTA, 1% NP-40, 1×protease inhibitor.

[0033] Phosphate buffer (10×PBS): 1.37M NaCl, 270mM KCl, 100mM NaCl 2 HPO 4 , 20mM KH 2 PO 4 The pH was adjusted to 7.4 with hydrochloric acid.

[0034] Protease inhibitor stock solution (50×): Dissolve one capsule of protease inhibitor cocktail in 1 mL of 1× PBS solution.

[0035] Reagents required for Western blot:

[0036] 5×S...

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Abstract

The invention relates to application of a polyphenol compound to preparation of a pharmaceutical for treating senile dementia, and relates to polyphenol compounds. The polyphenol compound is derived from a compound of miyabenol C of vitis thunbergii with nerve protection effects. The polyphenol compound can be used for preparing the pharmaceutical for treating senile dementia and can be obtained from Chinese traditional medicine or natural medicine through many separation and purification methods. The protein expression quantity of beta-CTFs and sAPPbeta (Sodium Acid Pyrophosphate) generated by beta-secretase (BACE-1) (Beta Amyloid Cleaving Enzyme) cutting APP can be reduced, and has obvious dose-effect relationship; and the cutting on APP can be reduced by inhibiting the activity of beta-secretase (BACE-1), the generation of Abeta 1-40 and Abeta 1-42 closely related to senile dementia is remarkably decreased.

Description

technical field [0001] The present invention relates to a polyphenolic compound, in particular to the application of a polyphenolic compound in the preparation of medicaments for treating senile dementia. Background technique [0002] Dementia, also known as Alzheimer's disease, is a neurodegenerative disease characterized by progressive memory loss and cognitive impairment. There are two main pathological features in Alzheimer's patients: extracellular amyloid plaques composed of β-amyloid (Aβ) and intracellular neurofibrillary tangles composed of hyperphosphorylated tau protein. As the average life expectancy of the world's population increases year by year, the global population tends to age, and the incidence of Alzheimer's disease also increases year by year, becoming a major hidden danger that threatens people's health in their later years. [0003] In recent years, the research and development of drugs for the treatment of Alzheimer's disease has attracted great atte...

Claims

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Application Information

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IPC IPC(8): A61K31/343A61P25/28
Inventor 陈海峰张云武徐士兰许华曦胥俊越胡进
Owner XIAMEN UNIV
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