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Alternate double-frequency countercurrent ultrasonic-assisted simultaneous extraction method for seaweed protein and polysaccharide mixed product

A technology of countercurrent ultrasound and extraction method, applied in the fields of food preparation, application, food science, etc., can solve the problems of single ultrasonic mode, it is difficult to obtain the effect of dissolution promotion and mass transfer enhancement, shorten the extraction time, etc., and achieve the mass transfer effect. Strong, shortened extraction time and high extraction efficiency

Inactive Publication Date: 2013-01-09
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ultrasound can use its cavitation, thermal and mechanical effects to improve the extraction efficiency of products, but the current ultrasonic-assisted extraction technology uses a single frequency ultrasonic mode
For the complex structures and forms of laver protein and polysaccharides, it is difficult to achieve the ideal effect of dissolution promotion and mass transfer enhancement by a single frequency of ultrasound, and it cannot well achieve the purpose of shortening the extraction time and improving the extraction yield. Development bottleneck, urgent need to find a solution

Method used

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  • Alternate double-frequency countercurrent ultrasonic-assisted simultaneous extraction method for seaweed protein and polysaccharide mixed product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Under the reference condition of ultrasonic power of 200W per liter volume, the extraction experiment of porphyra protein and polysaccharide was carried out by using two frequency ultrasonic waves. Crush laver flakes to 40 mesh, take 60 g of laver powder, dissolve in 6 L of tap water at a material-to-liquid ratio of 10 mg / mL, heat to 80 °C, adjust the pH to 9.0, and perform alternate dual-frequency countercurrent ultrasonic-assisted extraction of laver protein and polysaccharides. Alternate dual-frequency countercurrent ultrasonic-assisted extraction conditions are: 15 kHz and 20 kHz dual-frequency alternate operation, ultrasonic alternate working time 3 s, extraction time 30 min, and the feed liquid passes through the ultrasonic probe in a countercurrent circulation. After the extraction, centrifuge at 5000rpm for 10min, take the supernatant, concentrate, and spray-dry it to obtain the mixed product of laver protein and polysaccharide. The total yield of laver protein a...

Embodiment 2

[0024] The test process is the same as in Example 1, the difference is that the solid-liquid ratio is 15mg / mL, the temperature is 80°C, the extraction time is 40min, the total yield of laver protein and polysaccharide is 48.18%, and the yield of protein and polysaccharide is respectively 24.18% and 24.00%.

Embodiment 3

[0026] The test process is the same as in Example 1, the difference is that the solid-liquid ratio is 20mg / mL, the temperature is 80°C, the extraction time is 50min, the total yield of laver protein and polysaccharide is 38.02%, and the yield of protein and polysaccharide is respectively 15.50% and 22.52%.

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Abstract

The invention provides an alternate double-frequency countercurrent ultrasonic-assisted simultaneous extraction method for a seaweed protein and polysaccharide mixed product and relates to the field of development and utilization of marine products. The alternate double-frequency countercurrent ultrasonic-assisted simultaneous extraction method comprises the following steps of: crushing dried seaweed into 40 meshes, and adding running water which is 10 to 20 times that of the weight of the seaweed powder into the seaweed powder for dissolving; regulating the temperature to be 60 to 100 DEG C, and regulating the pH value to be 8.0 to 10.0; extracting under the reference conditions of ultrasonic power of 200W in volume per liter by employing an alternate double-frequency countercurrent ultrasonic-assisted water extraction method, performing double-frequency alternate operation at the specific parameters of 15kHz and 20kHz, wherein the ultrasonic alternate working time is 1 to 5 seconds, and the extraction time is 30 to 80 minutes, and the feed liquid passes through an ultrasonic probe in a countercurrent cycle mode; and centrifugally extracting the supernatant, concentrating the supernatant, spraying and drying to obtain the product when the extraction is ended. Compared with the conventional extraction method, the alternate double-frequency countercurrent ultrasonic-assisted simultaneous extraction method provided by the invention is high in exaction efficiency; and compared with a single-frequency ultrasonic-assisted extraction method, the alternate double-frequency countercurrent ultrasonic-assisted simultaneous extraction method provided by the invention has the advantages of good wall-breaking and mass transfer effects and high energy utilization rate.

Description

[0001] technical field [0002] The invention discloses an alternate dual-frequency countercurrent ultrasonic-assisted simultaneous extraction method for a mixed product of laver protein and polysaccharide, relates to the development and utilization of marine products, and belongs to the technical field of deep processing of laver. technical background [0003] Striped laver ( Porphyra yezoensis ) is an edible seaweed product with high nutritional value. The research found that Porphyra zebra has high protein and total sugar content (40.22% and 34.07%), and it is a raw material for the production of high-quality protein and polysaccharides. Laver protein and polysaccharides have good health care effects, such as laver polysaccharides have significant effects in reducing blood fat, anticoagulation, antithrombosis, and hypoglycemia; laver phycoerythrin is an important physiologically active substance. Therefore, there is currently an increasing interest in laver protein and ...

Claims

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Application Information

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IPC IPC(8): A23L1/337A23L1/09A23L17/60
Inventor 马海乐曲文娟任晓锋王婷
Owner JIANGSU UNIV
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