Compound biological agent for deodorizing public toilets, and preparation method and application of compound biological agent

A technology for biological preparations and public toilets, applied in the field of applied biology, can solve the problems of difficulty in exerting the complementary deodorization performance of plant extracts and microbial inoculants, expensive raw materials, and slow deodorization effect, so as to achieve in-situ source control of odors. , The effect of reducing the intensity of odor release and improving the toilet environment

Active Publication Date: 2013-01-09
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological deodorants mainly include plant extracts, microbial agents, or compound preparations mixed with plant extracts or essences and microbial agents. Most of the plant extract deodorants sold on the market are made from Chinese herbal medicines, bamboo, etc. Plant extracts have expensive raw materials, complex extraction processes, and high costs; while simple microbial agents have slow deodorizing effects and are difficult to work in a short period of time; compound preparations are mostly plant extracts or essences and microbial bacteria. It is difficult to exert the complementary deodorizing effect of plant extracts and microbial agents by simple mixing of agents

Method used

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  • Compound biological agent for deodorizing public toilets, and preparation method and application of compound biological agent
  • Compound biological agent for deodorizing public toilets, and preparation method and application of compound biological agent
  • Compound biological agent for deodorizing public toilets, and preparation method and application of compound biological agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Quantitatively prepare the MRS solid medium that has been sterilized at high temperature (121°C, 30 minutes). The formula of the MRS solid medium is 10g of peptone, 10g of beef extract, 5g of yeast extract, 5g of glucose, 5g of sodium acetate, and citric acid. Diammonium 2g, Tween 801mL, magnesium sulfate 0.58g, manganese sulfate 0.05g, dipotassium hydrogen phosphate 2g, agar 15-20g, add water to 1000mL, pH 6.2.

[0045] (2) Introduce strains ZW and SR into sterilized (121°C, 30 minutes) MRS solid slant medium for activation respectively.

[0046] (3) Quantify the liquid medium that has been sterilized at high temperature (121°C, 30 minutes) for later use. The formula of the liquid medium is 10g of peptone, 10g of beef extract, 5g of yeast extract, 5g of glucose, 5g of sodium acetate, dicitric acid Ammonium 2g, Tween 801mL, magnesium sulfate 0.58g, manganese sulfate 0.05g, dipotassium hydrogen phosphate 2g, add water to 1000mL, pH 6.2.

[0047] (4) Put the activate...

Embodiment 2

[0054] Add 10ml of liquid compound biological preparation to a beaker (1L) containing 400g of fresh feces, and seal the beaker with plastic wrap; at the same time, do a control without adding bacteria agent, and set up three repetitions for each group of experiments, and measure the odor intensity and ammonia concentration after 5 days , hydrogen sulfide concentration and TVOC concentration, the measurement results are as follows in Table 1:

[0055] Table 1: Deodorizing effect of compound biological preparations

[0056]

[0057] It can be seen from the above table that after adding bacterial agents to the feces, the odor intensity, ammonia concentration, hydrogen sulfide concentration and TVOC concentration were all significantly lower than those of the control, of which the odor intensity decreased by 38.8%, the ammonia concentration decreased by 44.3%, and the hydrogen sulfide concentration decreased by 38.8%. Reduced by 33.8%, TVOC concentration decreased by 35.8%.

Embodiment 3

[0059] Add 100g of solid compound biological agent (after activation) to a beaker (2L) containing 1000g of fresh feces, mix it with the feces, and seal the beaker with plastic wrap; at the same time, do a control without adding bacterial agents, and set three replicates for each group of experiments , measure stench intensity, ammonia concentration, hydrogen sulfide concentration and TVOC concentration after 5 days, measurement result is as follows table 2:

[0060] Table 2: Deodorizing effect of bacteria agent

[0061]

[0062] It can be seen from the above table that after adding bacterial agents to feces, the odor intensity, ammonia gas concentration, hydrogen sulfide concentration and TVOC concentration were all significantly lower than those of the control, of which the odor intensity decreased by 41%, the ammonia gas concentration decreased by 49.6%, and the hydrogen sulfide concentration decreased by 41%. Reduced by 40.1%, TVOC concentration decreased by 38.7%.

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Abstract

The invention discloses a compound biological agent for deodorizing public toilets. The compound biological agent contains a lactic acid bacteria: Lactobacillus sp. With the preservation number of CGMCC NO5983 and a lactic acid bacteria: Streptococcus thermophilus with the preservation number of CGMCC NO5985. The invention further discloses a preparation method of the compound biological agent for deodorizing the public toilets, namely the preparation method of compound biological agent and compound solid biological agent, as well as use of the compound biological agent.

Description

technical field [0001] The invention belongs to the field of applied biotechnology, and in particular relates to a compound biological preparation for deodorizing public toilets and a preparation method thereof. Background technique [0002] With the rapid development of my country's economy and the increasing frequency of population flow, especially the rapid development of tourism, the number of urban public toilets and public toilets in scenic spots is increasing, which has become an important symbol of the management level and civilization of cities and scenic spots. However, the stench pollution caused by it has become an important topic of environmental protection in cities and scenic spots. [0003] At present, deodorants for public toilets mainly include chemical deodorants and biological deodorants. Although chemical deodorants are highly targeted and effective, their application range is small. The odor effect will be invalid, and the use cost is high, and it is e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L9/013C12N1/20C12R1/225C12R1/46A61L101/56A61L101/52
Inventor 闫志英刘晓风袁月祥廖银章李志东许力山
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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