Method for separating and purifying daptomycin

Abstract

The invention discloses a method for separating and purifying daptomycin. The method comprises the steps of allowing daptomycin to form micelles, filtering by a ceramic membrane system, performing separation and purification and discoloring treatment by using a weak-base anion exchange resin separating system and a macroporous resin separating system, crystallizing, and thus a daptomycin solid with a chromatographic purity higher than 98% is obtained. The method is simple and practicable, is low in cost, and is suitable for industrialized production.

Description

technical field

[0001] The invention relates to a preparation method of daptomycin, in particular to a method for preparing high-purity daptomycin by using ceramic membrane filtration, resin chromatography and crystallization techniques. Background technique

[0002] With the development of antibiotics and the abuse of antibiotics, the resistance of pathogenic bacteria to antibiotics is the most severe challenge facing society today. In addition to controlling the abuse of antibiotics, finding an effective antibiotic against drug-resistant bacteria has become the best way to solve this problem. Vancomycin was once considered the last line of defense against Gram-positive bacteria, but now it is More and more bacteria resistant to this drug have been found clinically in the world.

[0003] Daptomycin is a lipopeptide antibiotic originally researched by Lilly and developed by Cubist Pharmaceuticals. In response to the urgent needs of patients for new drug-resistant antibioti...