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Technique for preparing (FOs) feruloyl oligosaccharides by adopting two-period-type combined regulation fermentation technology

A technology of feruloyl oligosaccharide and joint regulation, applied in the directions of fermentation, enzymes, biochemical equipment and methods, etc., can solve the problems of poor product safety, cumbersome production process and high production cost, so as to simplify the production process and save the Preparation steps, the effect of reducing production costs

Inactive Publication Date: 2013-01-16
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a two-stage joint regulation and control fermentation technology for preparing FOs, so as to solve the technical problems of cumbersome production process, high production cost, poor product safety and low yield in the current Fos production process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Wheat bran is naturally dried, crushed and passed through a 60-mesh sieve to prepare a fermentation medium, which consists of wheat bran 60g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 1g / L, VB 1 0.1g / L, sterilized at 0.103MPa for 20min; adjust the initial pH of the fermentation broth to 3.0, inoculate the activated Aphrodisiac syringae strain with an inoculation amount of 4.5%, and carry out liquid submerged fermentation at 32°C; after 24 hours of fermentation, adjust the fermentation broth The pH was 6.0, and the fermentation continued for 72 hours at 28°C, and the FOs production reached 894nmol / L.

Embodiment 2

[0022] The preparation of the fermentation medium is the same as in Example 1. Adjust the initial pH of the fermentation broth to 4.0, inoculate the activated Aureobasidium pullulans strain with an inoculation amount of 5%, and carry out liquid submerged fermentation at 33°C; after 24 hours of fermentation, adjust the pH of the fermentation broth to 6.0, and continue to ferment at 27°C for 72h, FOs The yield reached 982nmol / L.

Embodiment 3

[0024] The preparation of the fermentation medium is the same as in Example 1. Adjust the initial pH of the fermented liquid to be 5.0, inoculate the mixed strains of the activated Aphrodisiac edulis and Aureobasidium pullulans, the ratio is 1:1, the inoculum size is 6%, and carry out liquid submerged fermentation at 33°C; after 36 hours of fermentation, adjust The pH of the fermentation broth was 6.0, and the fermentation was continued at 29°C for 84 hours, and the FOs production reached 1012nmol / L.

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Abstract

The invention relates to a technique for preparing (FOs) feruloyl oligosaccharides by adopting a two-period-type combined regulation fermentation technology, belonging to the technical field of deep processing of agricultural and sideline products. The technique is characterized by comprising the following steps of: preparing a fermentation medium by adopting wheat bran as a main material, and regulating the initial pH and the initial temperature of fermentation liquor and then inoculating agrocybe aegevita and / or aureobasidium pullulans; and during the fermentation process, regulating the pH and the temperature of the fermentation liquor again, hydrolyzing wheat bran fibers by utilizing xylanase produced by microbes, so as to prepare the FOs. With the adoption of the technique, the reduction of the steps for preparing the FOs by adopting an enzymic method is facilitated; and the technique has the advantages of simple preparation process, low preparation cost and short preparation period, and can be used for improving the utilization ratio of wheat bran resources. Due to the adoption of the two-period-type combined regulation technology, the activity of the xylanase is ensured to be at the optimum level, so that the purpose of high yield of FOs is achieved. The technique is suitable for preparation of health-food-level FOs, and the yield of the FOs can reach 1123nmol / L.

Description

1. Technical field [0001] The invention relates to a process for preparing feruloyl oligosaccharides (FOs) by a two-stage combined regulation and fermentation technology, and belongs to the technical field of deep processing of agricultural and sideline products. 2. Background technology [0002] Functional oligosaccharides are a kind of sugar compounds with sweet and sticky properties, which can proliferate beneficial intestinal bacteria, prevent cancer, prevent obesity, prevent constipation, etc., and have been used as a substitute for sucrose. Feruloyl oligosaccharides (FOs), which widely exist in grasses, are a kind of functional oligosaccharides, which are esterified by sugar hydroxyl groups at different positions and ferulic acid (Ferulic acid, FA) carboxyl groups form. FOs have a feruloyl group and a hydrophilic oligosaccharide group, so they have both water solubility and heat resistance, and better solve the problem of being insoluble in water (such as carotene, ph...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12N9/42C12R1/645
Inventor 顾振新余晓红尹永祺屠康
Owner NANJING AGRICULTURAL UNIVERSITY
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