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Building method for medicament transportation or radiography carrier containing coat protein and inorganic nanoparticles

A technology of inorganic nanoparticles and shell proteins, applied in the field of viral proteins, to achieve the effect of improving biocompatibility

Inactive Publication Date: 2013-01-23
苏州百拓生物技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are some problems with viral vectors, such as the integration of the genes of the virus itself with the genome of the host, thus causing some potential diseases

Method used

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  • Building method for medicament transportation or radiography carrier containing coat protein and inorganic nanoparticles
  • Building method for medicament transportation or radiography carrier containing coat protein and inorganic nanoparticles
  • Building method for medicament transportation or radiography carrier containing coat protein and inorganic nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Gold nanoparticles induce self-assembly of rotavirus coat protein VP6

[0023] The synthesis of the gold nanoparticles used in this example is based on chloroauric acid as the raw material, and polyethyleneimine (PEI) as the reducing agent. Since PEI has amino groups and positive charges on it, the gold obtained after the reaction is completed The surface of the nanoparticle is positively charged. Depending on the reducing agent PEI and the reaction time, gold nanoparticles with different particle sizes can be obtained. Characterized by TEM, as figure 2 Shown: PEI is used as a reducing agent to synthesize gold nanoparticles, and after washing and centrifugation, gold nanoparticles with a particle size of 10nm to 30nm are obtained.

[0024] Such as figure 1 As shown, the method for gold nanoparticles to induce the self-assembly of rotavirus coat protein VP6 is as follows: first, the surface of gold nanoparticles is positively charged, and the modification ...

Embodiment 2

[0025] Example 2: A composite drug-loading system in which silicon dioxide-coated iron ferric oxide nanoparticles and viral proteins are combined

[0026] In this example, after ferric oxide is coated with silicon dioxide, pores are formed on the surface of silicon dioxide, so that the anticancer drug doxorubicin can be carried in the pores, and ferric oxide can play a role in magnetic targeting. . Due to the positive charge on the doxorubicin, the surface of the silica can be positively charged, thereby positively modifying the silica-coated iron ferric oxide nanoparticles, which is characterized by TEM, such as Figure 4 As shown, ferric ferric oxide nanoparticles are coated with silica, porogens are used to cause pores in the silica layer, and positively charged doxorubicin is adsorbed to the silica pores by physical adsorption. Formation of nanoparticles with positive surface charges for inducible assembly of viral proteins. The specific method is as follows: take the Fe...

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Abstract

The invention discloses a building method for a medicament transportation or radiography carrier containing coat protein and inorganic nanoparticles, comprising the following steps of: firstly, carrying out positive electricity modification on the surfaces of inorganic nanoparticles; and inducing viral protein to be assembled per se around the positive electricity-modified nanoparticles. According to the building method, the viral protein is induced to be assembled per se by the nanoparticles of which the surfaces are modified by the positive electricity, so that the nanoparticles of which the outer surfaces are coated with the coat protein can be obtained, and the biocompatibility, the stability in the blood, the cycle time in vivo of the nanoparticles and the like can be improved due to the modification of the viral protein, and therefore, the building method is more suitable for use in vivo; and the natural assemble mode of the viral protein can be changed due to the nanoparticles, so that a novel strategy can be found for the fundamental research, the vaccine development and the like of the virus.

Description

technical field [0001] The invention belongs to the technical field of viral proteins, and in particular relates to a method for inducing the self-assembly of viral proteins by inorganic nanoparticles, thereby constructing and obtaining a drug delivery or imaging carrier. Background technique [0002] Viruses, as the most genetically diverse organisms on earth, are good at folding long genomes through motor molecules, and then loading them into shells to form mature viruses. Viruses generally have two ways to load the genome: 1. The phage first folds the gene through a motor protein complex at the head, and then assembles into a mature virus; 2. Cowpea Chlorotic Mottle Virus (CCMV) Assembly method: assemble while compressing the genome, protein and gene interact and fold, and other proteins are assembled at the same time. In the viral coat structure protein, there is often a very conserved structure, that is, eight antiparallel β-sheet structures and loops linking these str...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61K49/14
Inventor 赵庆欢刘红海乐晓桐李咏梅顾莉娟
Owner 苏州百拓生物技术服务有限公司
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