Bacillus subtilis HAS and application thereof in preventing and treating sugarcane smut

A technology of Bacillus subtilis and smut, applied in the field of microorganisms, can solve problems such as sugarcane smut that has not yet been seen, and achieve the effect of reducing losses

Inactive Publication Date: 2013-01-30
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] After searching, there is no report on the successful use of microorganisms and their biocontrol agents for the prevention and treatment of sugarcane smut

Method used

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  • Bacillus subtilis HAS and application thereof in preventing and treating sugarcane smut

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Experimental program
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Effect test

Embodiment 1

[0017] Screening and identification of antagonistic bacteria of plant pathogenic fungi

[0018] Screening of bacterial strains: Weigh 5 grams of rhizosphere soil (the rhizosphere soil of sugarcane plants in the plot where sugarcane smut occurs seriously in Lingao County, Hainan Province, my country), put it into a triangular flask with 100ml sterile water, and put it on a shaker. After shaking vigorously for 30 minutes, let stand for 15 minutes. Take 1ml and dilute to 10 -4 、10 -5 、10 -6 Concentration gradient, take 0.1ml from each concentration gradient suspension, and spread it on LB medium with a sterile stick (the formula is: each liter contains 10g of peptone, 5g of yeast powder, 10g of NaCl, 12g of agar powder, pH7.0~7.2) On the plate, cultured upside down in a constant temperature incubator at 30°C, observed continuously for 3 days, picked strains with different colony shapes, streaked again, purified and stored for later use. Coat the teliospores of sugarcane smut o...

Embodiment 2

[0022] Determination of the control effect of Bacillus subtilis HAS on smut of sugarcane potted seedlings

[0023] Bacillus subtilis (Bacillus subtilis) HAS is used as the bacterial species to obtain biological preparations (the following are weight percentages):

[0024] 1. Solid culture: inoculate the preserved Bacillus subtilis HAS in solid medium (1% tryptone, 0.5% yeast extract, 1% NaCl, 1.2% agar powder, pH7.0.) , and cultured in a 37°C incubator for 1 day to activate the strain.

[0025] 2. Liquid culture: Inoculate the activated Bacillus subtilis HAS into a liquid medium (soybean flour 2.0%, corn flour 1.0%, sucrose 0.5%, peptone 0.3%, CaCO 3 0.2%, (NH 4 ) 2 SO 4 0.05%, MgSO 4 . 7H 2 O 0.02%, KH 2 PO 4 0.1%, NaOH 0.05%, peanut oil 0.2%, defoamer 0.02%, pH 6.8-7.2.) in 500ml Erlenmeyer flasks, each containing 200ml, cultured at 37°C for 36h on a shaker with a rotation speed of 170rpm to obtain liquid biological Preparation (biocontrol bacteria liquid one). ...

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Abstract

The invention relates to Bacillus subtilis HAS and an application thereof in preventing and treating sugarcane smut. The bacterial strain is named as Bacillus subtilis HAS, and is preserved in the general microorganism center of the Chinese microorganism bacterial strain preservation and administration committee, and the preservation number is CGMC No.6590. The use of the Bacillus subtilis HAS provided by the invention is the application of preventing and treating sugarcane smut; and a pot experiment shows that the Bacillus subtilis HAS has very good suppression function to sugarcane smut, the prevention effect can be 100% due to soaking seed, and the experiment shows that the bacterial strain is a biocontrol strain having potential meaning in preventing and treating sugarcane smut germs.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a bacterial strain and its application, in particular to a Bacillus subtilis HAS and its application in preventing and treating sugarcane smut. Background technique [0002] Sugarcane smut was first discovered in Natal, South Africa in 1877, and there have been reports of the disease in other countries and regions. China discovered the disease in Guangzhou in 1932, and the disease also appeared in various places afterwards. In recent years, with the popularization of vegetative propagation techniques of sugarcane species, frequent introduction and transportation of sugarcane species, long-term continuous cropping of the same variety in sugarcane fields and the gradual extension of the life of ratoon cane, resulting in the occurrence of sugarcane smut in Guangxi, Yunnan, Guangdong, Fujian, and Hainan in my country. The occurrence of sugarcane areas in other provinces is becom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
Inventor 熊国如伍苏然张树珍杨本鹏冯翠莲蔡文伟王俊刚王文治
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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