Method for determining content of calcium carbonate in reconstituted tobacco
A technology for reconstituted tobacco leaves and determination methods, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of analyzing the calcium carbonate content in reconstituted tobacco leaves without ion chromatography, and achieve good application prospects, good repeatability, and high sensitivity. Effect
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[0024] 1. Sample pretreatment
[0025] Grind the reconstituted tobacco leaf sample into powder and pass through a 40-mesh sieve, accurately weigh 0.1g (accurate to 0.0001 g) of the sample, place it in a 250mL conical flask, and accurately add 100mL, 1% acetic acid aqueous solution or 100mL of deionized water, Shake at room temperature for 30 minutes (120 rpm). Filter the extract (acid solution extraction needs to be diluted 10 times) through a 0.22μm filter membrane and transfer to a 2mL sample bottle for calcium ion analysis.
[0026] 2. Chromatographic analysis conditions
[0027] Cation exchange column DIONEX Ion Pac? CS12 (4 mm i.d × 50 mm); cation protection column Ion Pac CG12 (4 mm i.d × 50 mm). The eluent was isocratic eluting with methanesulfonic acid (MSA), the eluting concentration was 20 mmol / L, and the flow rate was 1.0 mL / min. Suppressor DIONEX CSRS 300 4 mm, suppression current: 59 mA; column temperature: 30 °C; injection volume: 50 μL.
[0028] Under the ab...
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