Method for preparing homoeriodictyol from mistletoe

A technology of high eriodiction and mistletoe, which is applied in the field of preparation of high eriodiction by enzymatic hydrolysis, can solve the problems of acid hydrolysis environmental pollution, unsuitable use, and unsuitable preparation of high ethiopia, and the method is simple to operate and solves the problem of The effect of heavy pollution

Inactive Publication Date: 2013-02-06
苏州宝泽堂医药科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Existing methods for preparing homo-election from mistletoe, no specific process
However, the method disclosed in the document "Study on the Chemical Components of Mistletoe I" is a research-type method, which is not suitable for the preparation of homo-elitorin
Due to the existence of glycosides in mistletoe, the traditional process generally uses acid hydrolysis, but acid hydrolysis will seriously pollute the environment and should not be used.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing homoeriodictyol from mistletoe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Grind mistletoe medicinal material, take 1kg, add 6 times the amount of 80% ethanol solution for microwave extraction twice, 1 hour each time, combine the extracts and concentrate alcohol-free hydrochloric acid to adjust ph5, add 3g cellulase and stir evenly, enzymolysis at 40-50°C for 10 hours , the enzymatic solution was added to the HZ816 macroporous resin column for adsorption, and eluted with 3 times the amount of 80% ethanol solution, the eluate was collected and concentrated under reduced pressure to crystallize, and filtered to obtain 13 g of crude extract. The crude extract dissolved in methanol was mixed with 30g polyamide to dry the sample, added to the polyamide resin column, first eluted with 4BV30% methanol, and then eluted with 50% methanol solution, and detected by TLC, and the high-concentration eluate was collected and concentrated to 1 / 2 3 Place the crystals on, filter out the crystals, recrystallize twice with 90% ethanol solution, and dry to obtain 7...

Embodiment 2

[0022] Grind mistletoe medicinal material, take 1kg, add 5 times the amount of 60% ethanol solution and ultrasonically extract 3 times, each time for 0.5h, combine the extracts with concentrated alcohol-free hydrochloric acid to adjust pH4.5, add 2g cellulase and 1g amylase and stir evenly, 40 Enzymolysis at -50°C for 8 hours, adding the enzymolysis solution to the AB-8 macroporous resin column for adsorption, eluting with 6 times the amount of 60% ethanol solution, collecting the eluate and concentrating the crystals under reduced pressure, filtering off to obtain 10g of crude extract . The crude extract dissolved in methanol was mixed with 20g polyamide to dry the sample, added to the polyamide resin column, first eluted with 3BV40% methanol solution, then eluted with 60% methanol solution, and detected by TLC, the high-concentration eluate was collected and concentrated to 1 / 2 4 Place the crystals on, filter out the crystals, recrystallize twice with 95% ethanol solution, a...

Embodiment 3

[0024] Grind mistletoe medicinal material, take 1kg, add 5 times the amount of 60% ethanol solution and ultrasonically extract 3 times, 0.5h each time, combine the extracts and concentrate alcohol-free hydrochloric acid to adjust pH5, add 1g cellulase, 1g amylase and 1g pectinase and stir Uniform, enzymolysis at 40-50°C for 10 hours, adding the enzymolysis solution to HPD-100 macroporous resin column for adsorption, eluting with 5 times the amount of 70% ethanol solution, collecting the eluate and concentrating the crystals under reduced pressure, filtering out to obtain crude Extract 11g. The crude extract dissolved in methanol was mixed with 30g polyamide to dry the sample, added to the polyamide resin column, first eluted with 4BV40% methanol, then eluted with 55% methanol solution, and detected by TLC, the high-concentration eluate was collected and concentrated to 1 / 2 3 Place the crystals on, filter out the crystals and recrystallize 3 times with 90% ethanol solution, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing homoeriodictyol from mistletoe. The method comprises the steps that: (1) a mistletoe medicine material is crushed; a 60-80% ethanol solution is added, and microwave or ultrasonic extraction is carried out 2-3 times; the extraction liquid is concentrated until no alcohol is left, and the pH value is regulated to 4-6; a biological enzyme is added for 8-10 hours of enzymolysis; an obtained enzymolysis liquid is absorbed by using macroporous resin column; 3-6 time amount of a 60-80% ethanol solution is used for eluting; an elution liquid is collected and is subjected to reduced-pressure concentration crystallization; and the material is filtered, such that a crude extract is obtained; and (2) the crude extract is dissolved by using methanol; polyamide is added and the mixture is stirred and dried; polyamide resin column is added; gradient elution is carried out by using a methanol solution; thin-layer detection is carried out; a high-concentration elution liquid is collected and is concentrated until a volume is small; the elution liquid is subjected to standing crystallization; an ethanol solution is used for re-crystallization 2-3 times; and the material is dried, such that homoeriodictyol is obtained. With the homoeriodictyol production method, the process is simple and is easy to operate, pollution to the environment can be reduced, and the method is suitable for industrialized productions.

Description

technical field [0001] The present invention relates to a method for preparing homo-election from mistletoe, in particular to a method for preparing homo-election by enzymatic hydrolysis. Background technique [0002] Mistletoe is also known as northern parasite, holly, mulberry parasite, willow parasite, yellow parasite, frozen green, and parasite. It usually parasitizes trees in the genus Quercus, Apple, Poplar, and Pinus. Modern studies have shown that mistletoe contains a large amount of flavonoids, alkaloids, and terpenoids, which have effects on the cardiovascular system, anti-platelet aggregation, and anti-tumor effects. [0003] Most of the flavonoids in mistletoe are flavonoid glycosides with homoeriotin as the mother nucleus, such as homoeriotin-7-O-β-D-glucoside, homoeriotin-7-O-β-D-glucoside -4-O-β-D-Apioside. [0004] Homoeriodictyol (homoeriodictyol), also known as homoeriodictyol, is a dihydroflavonoid compound, melting point: 223-224°C, molecular formula: C...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/32C07D311/40
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap