Preparation method of algistatic active substance di-isobutoxyphenyl amine

Abstract

The invention relates to a preparation method of an algistatic active substance di-isobutoxyphenyl amine, and relates to a phenyl amine compound having strong algicdal activity effect. The method comprises the following steps of: preparing fermentation liquid by brevibacterium sp. BS01, centrifuging the fermentation liquid and then adding an extracting agent, evaporating the extract, adding methanol for dissolving and centrifuging, desalting and deproteinizing to obtain a crude extract; dissolving the crude extract in the extracting agent, eluting, collecting eluate, performing chromatography, performing ultraviolet coloration, iodine vapor coloration and ammonium sulfate ethanol coloration, mixing similar components to obtain mixed eluate; evaporating and dissolving the evaporated eluate in DMSO (dimethylsulfoxide) to verify algistatic activity and selecting a crude extract containing components with algicdal activity; separating the crude extract by a sephadex column; carrying out chromatographic analysis, performing ultraviolet coloration, iodine vapor coloration and ammonium sulfate ethanol coloration, mixing similar components to obtain mixed eluate, evaporating and dissolving evaporated eluate in DMSO to verify algistatic activity and selecting an active substance containing components with algicdal activity; and performing chromatography and <1>H-NMR (nuclear magnetic resonance) mass spectrometric detection to obtain a product.

Description

technical field [0001] The invention relates to an aniline compound with strong algicidal activity, in particular to a preparation method of an algae-inhibiting active substance diisobutoxyphenylamine. Background technique [0002] Marine actinomycetes are a special class of microorganisms with important economic value. In recent years, more than 50% of the newly discovered marine microbial active substances are produced by the huge taxa of marine actinomycetes, making it possible to use actinomycetes to control water Hua and red tides have become areas worthy of further study. With the widespread use of molecular ecology methods, the research on the diversity of marine actinomycetes, especially the diversity of deep-sea actinomycetes has made great progress, and more and more marine actinomycetes have been discovered, including new 16SrDNA sequences Clusters, the species and number of actinomycetes increased continuously, from the initial 3 genera to 170 genera, however, i...

AI Technical Summary

Problems solved by technology

With the widespread use of molecular ecology methods, the research on the diversity of marine actinomycetes, especially the diversity of deep-sea actinomycetes has made great progress, and more and more marine actinomycetes have been discovered, including new 16SrDNA sequences Cluster, the species and number of actinomycetes are increasing, from the initial 3 genera to 170 genera, however, its known species are still insignificant compared with their estimated number of existence in nature

Images