Synchronous fluorimetry for simply measuring pyrene in fish gills

A technique of synchronous fluorescence and fish gills, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of many experimental links, complex instruments, high cost, etc., and achieve less scattered light interference, simple instruments and equipment, and low detection limit Effect

Inactive Publication Date: 2013-02-06
HUBEI POLYTECHNIC UNIV
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Problems solved by technology

When using classic and traditional GC-MS methods to measure pyrene in fish gill tissue, th

Method used

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  • Synchronous fluorimetry for simply measuring pyrene in fish gills
  • Synchronous fluorimetry for simply measuring pyrene in fish gills
  • Synchronous fluorimetry for simply measuring pyrene in fish gills

Examples

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Embodiment Construction

[0019] 1. Fish gill sample pretreatment (freeze drying, grinding, extraction, purification)

[0020] Take out 6 fish from the 20-100ppb pyrene exposure pool, stun the head, dissect the fish gills quickly after bloodletting, take the gills one by one, merge the gills on both sides, and dry the fish gills for 24 hours. Mix with 3 grams of anhydrous sodium sulfate in a bowl and grind to powder. Put the above powder sample into the ASE column, and extract pyrene from fish gills by using the rapid solvent extractor (ASE). ASE extraction conditions: temperature: 125°C (heating for 5min); pressure: 1500psi; static extraction time: 10min; cycle times: 2 times; extraction solvent is n-hexane:dichloromethane (1:1). Both n-hexane and dichloromethane are analytical reagents, which are used after re-distillation. After ASE extraction, the above extract was rotovaped. The concentrated solution obtained was passed through a silica gel chromatography column, and the chromatography column w...

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Abstract

The invention discloses a synchronous fluorimetry for simply measuring pyrene in fish gills. The method comprises the steps of: airing, grinding and extracting a fish gill sample, rotatably evaporating and concentrating until the fish gill sample is almost dried before purifying, fixing the volume to measure by normal hexane, taking the normal hexane as a solvent to prepare pyrene standard solution of which the concentration range is 1-1000ug/l, measuring simultaneous wavelength EX334.5nm/delta lambda 50nm; measuring pyrene standard series of samples and to-be-measured samples with fixed volume by the synchronous fluorimetry, formulating a standard curve equation, and carrying out quantitative calculation on the to-be-measured samples according to the standard curve equation. The synchronous fluorimetry has the following advantages that (1) measurement is carried out by the synchronous fluorimetry, and the apparatus is relatively simple, easy to operate and low in measurement cost; (2) pyrene measurement in the fish gills is carried out by the synchronous fluorimetry, and the recovery rate is high; (3) an optimized measurement parameter is adopted as to EX and delta lambda; the measurement selectivity is improved; and the standard curve equation is high in linearity and low in detection limit.

Description

technical field [0001] The invention relates to an analysis and detection method for polycyclic aromatic hydrocarbons in environmental samples, in particular to a synchronous fluorescence method for measuring pyrene in fish gills. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the aquatic environment. Pyrene is used as a representative PAHs compound due to its moderate molecular weight and co-carcinogenicity. Also because the content of pyrene in environmental media often has good correlation characteristics with the total amount of PAHs, pyrene has become one of the model compounds often used in related research. In rivers and rivers, since fish is an important food chain in the ecological environment and one of the main food sources for humans, the study of the enrichment and release behavior of organic pollutants in fish can provide an important basis for ecological risk assessment. Fish gills are tissues and organs that effectiv...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 刘先利陶澍
Owner HUBEI POLYTECHNIC UNIV
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