Actin binding peptide and purpose thereof
A technology of actin and peptide binding, applied in the field of medicine, can solve problems such as unsuitable for large-scale batch production, technical difficulty and high cost, and unsatisfactory treatment effect, so as to promote corneal wound healing and transparency restoration, and difficult synthesis And the effect of cost reduction and superior repairing effect
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Embodiment 1
[0017] Embodiment 1, the preparation of polypeptide LKKTETQ eye drops of the present invention
[0018] Using water as a solvent, sodium chloride and potassium chloride as an osmotic pressure regulator, calcium chloride and magnesium chloride as an electrolyte, and sodium hydroxide as a pH regulator, through a buffer solution containing sodium acetate and sodium citrate, the preparation containing the main drug LKKTETQ eye drops.
[0019] Weigh 6.4g of sodium chloride, 0.75g of potassium chloride, 0.48g of calcium chloride, 0.3g of magnesium chloride, 3.9g of sodium acetate, and 1.7g of sodium citrate into a container, add about 900ml of sterile water for injection, stir to dissolve ; Then add 0.02g actin-binding peptide (LKKTETQ peptide) and mix well, use 0.1mol / L sodium hydroxide solution to adjust the pH to 7.0, and finally add sterile water for injection to 1000ml. The solution was filtered through a sterile membrane, and filled with a sterile polypropylene bottle to obta...
Embodiment 2
[0020] Example 2, LKKTETQ repair test on rat corneal epithelium and matrix refractory damage after lamellar resection
[0021] Clean grade SD rats, 8-9 weeks old, male.
[0022] Rat half-thickness keratectomy model was made: rats were anesthetized by intraperitoneal injection of 1.5% pentobarbital sodium, the right eye was selected as the operated eye, and the corneal epithelium and about 1 / 2 of the depth of the corneal stroma were completely removed with a scalpel.
[0023] Eight lamellar excision model rats were randomly divided into 2 groups, 4 in each group, and LKKTETQ eye drops (specification: 1ml: 0.02mg) and recombinant human epidermal growth factor eye drops (trade name: Yibei, Produced by Guilin Huanuowei Gene Pharmaceutical Co., Ltd., 5ml / bottle), 1 drop / time (equivalent to about 0.03ml), administered 3 times a day at 8:00, 13:00, and 19:00. In the case of corneal perforation in the operated eye during the operation, or corneal ulcer or perforation in the observed ...
Embodiment 3
[0027] Embodiment 3, the research of LKKTETQ to the repairing effect of rabbit eye cornea
[0028] 20 white healthy rabbits, half male and half male, body weight 2±0.1kg / only, were kept in single cages during the experiment.
[0029] A circular wound surface with a diameter of 10mm was artificially created by corneal epidermis scraping and grinding, and the wound surface showed yellow-green fluorescence when examined by sodium fluorescein solution.
[0030] The animals were randomly divided into 5 groups, 4 in each group, half male and half male. The same group of animals was treated with the same concentration of LKKTETQ, and the experimental continuous treatment started 1 h after the wound formation. The right eyes of all animals were used as controls, 2 times a day, 1 drop of normal saline each time; the left eyes were repaired, 2 times a day, 1 drop of LKKTETQ solution each time, until the corneal wound was completely healed.
[0031] The change of corneal trauma area wa...
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