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Method for highly efficiently extracting protein from walnut dreg

A technology for walnut meal and walnut meal powder is applied in the field of high-efficiency extraction of walnut protein with reverse micelle combined with microwave and enzymatic methods, which can solve the problems of extracting walnut protein without a reverse micelle system, and achieves low cost, low production cost, and improved extraction. rate effect

Inactive Publication Date: 2013-02-20
新疆林科院经济林研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report on the extraction of walnut protein using the reverse micellar system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] a. Preparation of walnut meal powder: the walnut meal obtained after walnut cold pressing is pulverized on a high-speed pulverizer, and after crushing, 120 mesh sieves are passed to obtain walnut meal powder, and its protein content is measured to be 47.85%;

[0031] b. Preparation of reverse micellar solution: Dissolve n-hexane in diisooctyl sodium sulfonate and mix After the mixed solution is transparent, mix the mixed solution with KCl-containing phosphate buffer solution according to the ratio of mixed solution: phosphate buffer solution with KCl concentration of 0.2 mol / L and pH of 7.0 = 1 mL: 0.01 mL to obtain reverse glue Beam solution, the mol ratio W of water and diisooctyl sodium sulfonate succinate in this reverse micelle solution 0 is 10;

[0032] c. Pre-extraction: Mix the walnut meal powder in step a and the reverse micellar solution in step b according to the ratio of reverse micellar solution: walnut meal powder = 1 mL: 0.10 g, and stir at 30°C for 90 m...

Embodiment 2

[0036] a. Preparation of walnut meal powder: the walnut meal obtained after walnut cold pressing is pulverized on a high-speed pulverizer, and after crushing, 80 mesh sieves are passed to obtain walnut meal powder, and its protein content is measured to be 47.85%;

[0037]b. Preparation of reverse micellar solution: Dissolve n-hexane in diisooctyl sodium sulfonate, and mix After the mixed solution is transparent, mix the mixed solution with the phosphate buffer solution containing KCl according to the ratio of mixed solution: phosphate buffer solution with a KCl concentration of 0.03 mol / L and a pH of 10.0 = 1 mL: 0.06 mL, to obtain reverse gum Beam solution, the mol ratio W of water and diisooctyl sodium sulfonate succinate in this reverse micelle solution 0 is 30;

[0038] c. Pre-extraction: mix the walnut meal powder in step a and the reverse micellar solution in step b according to the ratio of reverse micellar solution: walnut meal powder = 1 mL: 0.02g, stir at 50°C for ...

Embodiment 3

[0042] a. Preparation of walnut meal powder: the walnut meal obtained after walnut cold pressing is pulverized on a high-speed pulverizer, and after crushing, 100 mesh sieves are passed to obtain walnut meal powder, and its protein content is measured to be 47.85%;

[0043] b. Preparation of reverse micellar solution: Dissolve n-hexane in diisooctyl sodium sulfonate and mix After the mixed solution is transparent, mix the mixed solution with the phosphate buffer solution containing KCl according to the ratio of mixed solution: KCl concentration of 0.21mol / L, pH 8.5 phosphate buffer solution = 1 mL: 0.04 mL, to obtain reverse glue Beam solution, the mol ratio W of water and diisooctyl sodium sulfonate succinate in this reverse micelle solution 0 is 20;

[0044] c. Pre-extraction: Mix the walnut meal powder in step a and the reverse micellar solution in step b according to the ratio of reverse micellar solution: walnut meal powder = 1 mL: 0.07g, stir at 40°C for 60 min, and the...

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Abstract

The invention provides a method for highly efficiently extracting protein from walnut dreg. The method comprises the steps that: a, walnut dreg is crushed into walnut dreg powder; b, according to a ratio that n-hexane:succinic diisooctyl sodium sulfonate=1mL:0.04-0.10g, n-hexane is dissolved in succinic diisooctyl sodium sulfonate, such that a mixed solution is obtained; the mixed solution is mixed with an electrolyte buffering solution, such that a reverse micelle solution is obtained; c, the walnut dreg powder in the step a and the reverse micelle solution obtained in the step b are mixed according to a ratio that reverse micelle solution:walnut dreg powder=1mL:0.02-0.10g; the mixture is subjected to stirring or microwave and enzyme method assisted extraction; centrifugation is carried out, and a supernatant is a protein pre-extraction liquid; d, the protein pre-extraction liquid obtained in the step c and the electrolyte buffering solution is mixed according to a volume ratio that protein pre-extraction liquid:electrolyte buffering solution=1:0.5-1:2; the mixture is subjected to stirring or microwave-assisted extraction; centrifugation is carried out, and lower-layer clear liquid is protein post-extraction liquid; and e, the protein post-extraction liquid obtained in the step d is subjected to chromatography column desalting, such that a product is obtained.

Description

technical field [0001] The invention relates to the technical field of walnut protein extraction, in particular to a method for efficiently extracting walnut protein by combining reverse micelles with microwave and enzymatic methods. Background technique [0002] Walnuts are rich in high-quality protein, the content of which is about 15%. The protein content of walnut meal after walnut oil production reaches more than 40%, and the content of essential amino acids is high, which can be compared with meat protein. It contains a variety of vitamins and minerals, does not contain cholesterol, and is an excellent food raw material. At present, domestic and foreign studies on walnuts focus on their oils, and there are few studies on the development and utilization of walnut proteins. Due to the processing characteristics of walnut, especially walnut protein is more sensitive to heat than other vegetable proteins, and the denaturation temperature is relatively low, which is 67°C. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14
Inventor 虎海防赵晓燕王宪昌刘凤兰孙丽陈锋亮
Owner 新疆林科院经济林研究所
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