Scenedesmus obliquus producing self-flocculation substance, and application of same in microalgae harvesting
A technology of Scenedesmus obliquus and self-flocculation, which is applied in the field of microbial applications, can solve the problems that the filtration method depends on the size of algal cells, is not suitable for large-scale microalgae collection, and is complicated in operation, and achieves good pH stability and thermal stability. Effect
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Embodiment 1
[0032] Embodiment 1: The cultivation of the self-flocculating Scenedesmus obliquus of the present invention.
[0033] The self-flocculating Scenedesmus obliquus of the present invention is derived from the freshwater body in southern Taiwan, and the most suitable culture conditions are as follows: the pH of the initial medium is 6.0-6.2; the initial concentration is about 0.05-0.1 at O.D.685nm; the culture temperature is 28°C; the light intensity 60 μmol m -2 the s -1 ; Photoperiod: 16h light culture, 8h dark culture. When cultured in the optimized DM liquid medium for 4-5 days, the algal cells are mostly in an aggregated state, and the size of the aggregates can be seen with the naked eye. After the 13-day culture medium is left still overnight, the algal cells aggregate together and settle into the Erlenmeyer flask At the bottom, the supernatant is clearly visible. Under the electron microscope, it was found that there is a membrane-like substance outside the algal cells ...
Embodiment 2
[0033] The self-flocculating Scenedesmus obliquus of the present invention is derived from the freshwater body in southern Taiwan, and the most suitable culture conditions are as follows: the pH of the initial medium is 6.0-6.2; the initial concentration is about 0.05-0.1 at O.D.685nm; the culture temperature is 28°C; the light intensity 60 μmol m -2 the s -1 ; Photoperiod: 16h light culture, 8h dark culture. When cultured in the optimized DM liquid medium for 4-5 days, the algal cells are mostly in an aggregated state, and the size of the aggregates can be seen with the naked eye. After the 13-day culture medium is left still overnight, the algal cells aggregate together and settle into the Erlenmeyer flask At the bottom, the supernatant is clearly visible. Under the electron microscope, it was found that there is a membrane-like substance outside the algal cells to wrap the cells and adhere together ( figure 1 ). Example 2: Genome extracted from Scenedesmus obliques and ...
Embodiment 3
[0047] Embodiment 3: the growth situation and the determination of biomass content of self-flocculation Scenedesmus oblique
[0048] The self-flocculating Scenedesmus obliquus was inoculated in the optimized DM liquid medium. After 5-13 days of culture, the algae grew rapidly, the number of algae cells increased rapidly, and the nitrogen content decreased significantly. The pH did not change much during the whole culture process, and the highest value was not More than 8, and no inorganic precipitates ( figure 2 ). After 16 days of cultivation, the protein content in the self-flocculating algae biomass can reach 28.4±1.3% of the dry weight, carbohydrates account for 25.3±1.0%, lipids account for 22.9±1.1%, and chlorophyll is as high as 13.0±0.6%, indicating that the algal strains of the present invention have Wide application prospects.
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