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Application of tea saponin and preparation thereof

A technology of tea saponin and inhibitor, which is applied in the field of application of tea saponin and its preparation, can solve the problems that have not been seen in the application of tea saponin, and achieve the effect of convenient material collection and broad application prospects

Inactive Publication Date: 2013-03-06
LB COSMECEUTICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, there is no relevant report on the application of tea saponin as a Malassezia inhibitor

Method used

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  • Application of tea saponin and preparation thereof
  • Application of tea saponin and preparation thereof
  • Application of tea saponin and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of tea saponin.

[0040] Tea seed cake was used as raw material, 70% ethanol aqueous solution was used as extract, mixed according to the mass ratio of 1:8, heated and refluxed at 85°C for 2 hours; D101 macroporous resin with 4 times the mass of tea seed cake was used After absorbing the extract, it was eluted with 0.2% sodium hydroxide solution until it was colorless; it was washed with distilled water until the pH of the solution was neutral, and then eluted with 90% ethanol water solution until it was colorless, and the eluate of 90% ethanol water solution was collected. Dry to get tea saponin.

Embodiment 2

[0042] Inhibition experiment of tea saponin (Zhejiang Dongfang Tea Technology Co., Ltd.) on Malassezia.

[0043] Experimental strain: Malassezia furfur (Malassezia furfur, ATCC44344);

[0044] Medium: Malassezia solid medium: glucose 40g / L, agar 14g / L, peptone 10g / L, dissolved in distilled water, adjusted to pH 6.8~7.0, autoclaved at 115°C for 20min; Malassezia liquid medium : Glucose 40g / L, dissolved in distilled water, adjusted to pH 6.8~7.0, autoclaved at 115°C for 20min.

[0045] Experimental method: After the test bacteria were subcultured on the Malassezia solid medium, to ensure the purification and viability of the colony, pick a single clone into 50ml Malassezia liquid medium, culture at 30°C for 24h, and use turbidimetric Instrument (BioMérieux) to adjust to the concentration of McFarland No. 4 turbidimetric tube.

[0046] Dilute the bacterial solution with the medium to make the final bacterial concentration 105CFU / mL, then draw 100ul and spread it on the solid me...

Embodiment 3

[0050] Determination of the minimum inhibitory concentration (MIC value) of tea saponin against Malassezia

[0051] Experimental strain: Malassezia furfur (Malassezia furfur, ATCC44344);

[0052] Medium: Malassezia liquid medium: glucose 40g / L, dissolved in distilled water, adjusted to pH 6.8~7.0, autoclaved at 115°C for 20min.

[0053] Experimental method: After the test bacteria were subcultured on the Malassezia solid medium, to ensure the purification and viability of the colony, pick a single clone into 50ml Malassezia liquid medium, culture at 30°C for 24h, and use turbidimetric Instrument (BioMérieux) to adjust to the concentration of McFarland No. 4 turbidimetric tube.

[0054] The tea saponin was directly diluted with Malassezia liquid culture medium to test concentrations, which were 10, 5, 2.5, 1.25, 0.625, 0.3125, 0.156, and 0.078 mg / ml, respectively. Add 100 μL of bacterial solution and 100 μL of medicinal solution to the microwells of a 96-well plate, and set u...

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Abstract

The invention discloses an application of tea saponin used as a malassezia inhibitor, and in particular relates to an application for preparing drugs for treating skin diseases caused by malassezia. The invention further discloses an external preparation and daily cosmetics containing the tea saponin.

Description

technical field [0001] The present invention relates to the application and preparation of tea saponin. Background technique [0002] Modern medical research has found that Malassezia (Pityrosporum) is a resident flora on the skin of humans and warm-blooded animals, belonging to the genus of lipophilic basidiomycetes. As early as the 1990s, people had found Malassezia furfur, Malassezia pachyderm, and Malassezia symaxis on the skin of warm-blooded animals; Malassezia globosa, Malassezia obtuse, Malassezia restricta and Malassezia slofi; said Malassezia mainly grows in the stratum corneum of human Lipid flora mainly feed on triacylglycerols and free fatty acids on the skin surface. The study also found that the strength of the sebaceous gland function is the main factor affecting the secretion of triacylglycerol and free fatty acid, so the growth density of Malassezia on the skin decreases due to aging and less lipid secretion. The said Malassezia grows in a yeast phase an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K8/63A61K9/12A61K9/06A61P31/04A61P17/00A61P17/06A61P17/08A61Q5/02A61Q5/12A61Q19/10A61Q19/00
Inventor 孙常磊朱丽平段朝辉翟春涛赵雪梅李成亮
Owner LB COSMECEUTICAL TECH CO LTD
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