Drug fusant of specific binding GLP-1 (Glucagon-like peptide-1) receptor
A technology of GLP-1 and fusion, applied in the field of genetic engineering, can solve the problems of reducing therapeutic effect, decreasing biological activity, causing immunogenicity, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0084] This example describes the preparation method of the drug fusion named HSK-B-01 in the present invention, the X fragment sequence of the fusion is:
[0085] HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS (SEQ ID NO: 1),
[0086] The sequence of X' fragments is:
[0087] SPPPAGSSPGGNKLWEIFLRVAEEEMQKSLDSTFTGEGH (SEQ ID NO: 7),
[0088] Y is GGGGGCGGGGG (SEQ ID NO: 4),
[0089] Z is a molecular weight of 20,000 Daltons, the end of which is maleic imide-activated monomethoxypolyethylene glycol,
[0090] a and b do not exist, that is, the number of amino acid residues is 0, n=1.
[0092] Cloning of plasmids expressing the following protein sequences:
[0093] HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPSGGGGGCGGGGG SPPPAGSSPGGNKLWEIFLRVAEEEMQKSLDSTFTGEGH (SEQ ID NO: 8)
[0094] Primer 5'-CATGCCATGGCCCATATGAAATACCTGCTGCCGACCGCTGC-3' (SEQ ID NO:9)
[0095] 5'-CCGAATTCTCATTAGTGGCCCTCGCCGGTGAAGGTGCTGT-3' (SEQ ID NO: 10)
[0096] The DNA sequence encodin...
Embodiment 2
[0107] Example 2 This example describes the preparation method of the drug fusion named HSK-B-02 in the present invention, the X fragment sequence of the fusion is:
[0108] HGEGTFTSDVSSYLEGQAAKEFIAWLVKGRG (SEQ ID NO:2),
[0109] The sequence of X' fragments is:
[0110] GRGKVLWAIFEKAAQGELYSSVDSTFTGEGH (SEQ ID NO: 12),
[0111] Y is GGGSGGGCGGGSGGG (SEQ ID NO:5),
[0112] Z is a molecular weight of 30,000 Daltons, the end of which is maleic imide-activated monomethoxypolyethylene glycol,
[0113] The amino acid residue sequence of a is DKTHT,
[0114] The amino acid residue sequence of b is PAPELL, n=1.
[0115] clone
[0116] Cloning of plasmids expressing the following protein sequences:
[0117] HGEGTFTSDVSSYLEGQAAKEFIAWLVKGRGGGGSGGGCGGGSGGG GRGKVLWAIFEKAAQGELYSSVDSTFTGEGH (SEQ ID NO: 13)
[0118] Primer 5'- CATGCCATGGCCCATATGAA ATACCTGCTGCCGACCGCTGC -3' (SEQ ID NO: 14)
[0119] 5'-CCGAATTCTCATTAGTGGCCCTCACCGGTGAAGGTGCTGT-3' (SEQ ID NO: 15)
[0120] The DNA s...
Embodiment 3
[0128] This example describes the preparation method of the drug fusion body named HSK-B-03 in the present invention, the X fragment sequence of the fusion body is:
[0129] HGEGTFTSDLSKQMEEEAVALPIETWLKQGGPSSGAPPSKKKKKK (SEQ ID NO: 3),
[0130] The sequence of X' fragments is:
[0131] KKKKKKSPPAGSSPGGQKLWTEIPLAVAEEEMQKSLDSTFTGEGH (SEQ ID NO: 17),
[0132] Y is GGGGGSGGGCGGGSGGGGG (SEQ ID NO: 6),
[0133] Z is a polyethylene glycol-polylactic acid block copolymer with a molecular weight of 20,000 Daltons and activated by maleic imide at the end,
[0134] a and b do not exist, that is, the number of amino acid residues is 0, n=1.
PUM
Property | Measurement | Unit |
---|---|---|
molecular weight | aaaaa | aaaaa |
molecular weight | aaaaa | aaaaa |
molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com