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Pseudomonas psychrophila HA-4 low-temperature sulfamethoxazole degrading bacteria as well as screening method and application thereof

A technology of sulfamethoxazole and degrading bacteria, applied in the fields of bioengineering and environmental engineering

Active Publication Date: 2014-07-23
宜兴环保产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

is a low-temperature Pseudomonas strain

Method used

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  • Pseudomonas psychrophila HA-4 low-temperature sulfamethoxazole degrading bacteria as well as screening method and application thereof
  • Pseudomonas psychrophila HA-4 low-temperature sulfamethoxazole degrading bacteria as well as screening method and application thereof
  • Pseudomonas psychrophila HA-4 low-temperature sulfamethoxazole degrading bacteria as well as screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The bacterial strain Pseudomonas psychrophila provided by the invention is separated from activated sludge, and is screened at a set temperature (10°C). The specific screening steps are as follows:

[0037] (1) Take 20mL of fresh activated sludge from the aerobic tank of Harbin Taiping Urban Sewage Plant and add 100mL of inorganic salt culture medium containing 20mg / L sulfamethoxazole as the sole carbon and energy source into a triangular flask at a temperature of 10 °C, cultured in a shaker with a rotation speed of 150r / min for one week to obtain the enriched bacterial liquid;

[0038] (2) Take 10mL of the enriched bacteria liquid and add it to a 100mL sulfamethoxazole inorganic salt culture medium conical flask, repeat the culture conditions of step (1) 3 to 4 times, each time the concentration of sulfamethoxazole is graded Improve, finally to 100mg / L, to obtain the domesticated bacterial liquid, and obtain the OD of the domesticated bacterial liquid 660 =1.192;

[...

Embodiment 2

[0053] The present invention uses sulfamethoxazole as the only carbon and nitrogen source medium to degrade it and strain it Pseudomonas psychrophila The application of HA-4 growth status research, its steps are as follows:

[0054] strain Pseudomonas psychrophilaHA-4 under the conditions of temperature 10°C, initial pH value 6.0, shaker speed 150r / min, 50mL Erlenmeyer flask liquid volume 20mL, the inoculum amount of HA-4 bacterial suspension was 15%, sulfamethoxazole The initial concentration of the degradation system was 100 mg / L, and the culture was continued for 192 hours, and the concentration of sulfamethoxazole was detected every 24 hours. Using high performance liquid chromatography, the chromatographic conditions are Waters e2695 Alliance HPLC, and the chromatographic column is Symmetry -C18 column (4.6×250 mm, 5 μm); the mobile phase is acetonitrile: formic acid water=40:60 (V / V); the detection wavelength is 265nm; the column temperature is 30°C; the flow rate i...

Embodiment 3

[0058] The present invention uses sulfamethoxazole as the only carbon and nitrogen source medium for its degradation research:

[0059] The initial concentration of the sulfamethoxazole degradation system will be changed in Example 2, and the other steps are the same as in Example 2. image 3 It is the degradation situation of HA-4 bacterial suspension to different initial concentration sulfamethoxazole; By image 3 Visible, strain Pseudomonas psychrophila HA-4 has efficient degradation ability to sulfamethoxazole, and can tolerate the concentration of sulfamethoxazole up to 200mg / L.

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Abstract

The invention discloses a pseudomonas psychrophila HA-4 low-temperature sulfamethoxazole degrading bacteria, and belongs to the technical fields of bioengineering and environment engineering technology. The pseudomonas psychrophila HA-4 low-temperature sulfamethoxazole degrading bacteria is a low-temperature pseudomonas strain, which is preserved by the microbial strains preservation management committee center of general microbiology in March 27th, 2012, with the preservation number of CGMCC No.5944. The pseudomonas psychrophila HA-4 low-temperature sulfamethoxazole degrading bacteria is obtained by separating sludge in some sewage treatment plant aeration tank in Haerbin city; the strain can be grown by serving sulfamethoxazole as unique carbon, nitrogen and energy sources; the sulfamethoxazole can be degraded during culture of cell liquid; the cell liquid can be used for biodegradation of the sulfamethoxazole in city sewage; and by the strain, the removal rate of the sulfamethoxazole with the initial concentration of 100mg / L under a low-temperature condition is up to 35.34 percent.

Description

technical field [0001] The invention belongs to the technical fields of biological engineering and environmental engineering, and relates to bacterial strains Pseudomonas psychrophila HA-4 and its application in the degradation of sulfamethoxazole. Background technique [0002] Pharmaceuticals and personal care products mainly include various prescription and non-prescription drugs, fragrances, hair dyes, cosmetics, sunscreens, hair sprays, shampoos, soaps, etc. Most of the drugs are directly discharged into the environment without being metabolized in the organism. In addition, they will also enter the water environment during the production process of the drug and the process of disposing of expired and unused drugs; personal care products will also be in the environment. Directly into the water environment when washing and swimming. [0003] Although the concentration of most PPCPs in the environment is very low, due to bioaccumulation, PPCPs will eventually have a pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/38C02F101/38
Inventor 马放蒋本超李昂蔡蕊崔迪邱天张斯庞长泷
Owner 宜兴环保产业有限公司