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Tissue culturing method for effectively improving general flavone content of tartary buckwheat

A technology of total flavonoids and tartary buckwheat, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of artificial hybridization, difficulty in success, difficulty in obtaining high-yielding and fine varieties of tartary buckwheat, and achieve the effect of increasing the content of total flavonoids

Active Publication Date: 2013-03-13
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the characteristics of self-pollination of tartary buckwheat plants, it is difficult to succeed in artificial hybridization in agricultural production and breeding, which is also an important reason why it is difficult to obtain high-yield and excellent varieties of tartary buckwheat in breeding.
At present, there is no report on the use of precursor feeding technology to significantly increase the total flavonoid content in tartary buckwheat tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Treatment of explants: select the 1st to 2nd young stem segments close to the terminal buds of tartary buckwheat plants that are healthy and free from diseases and insect pests as explants, sterilized with 0.1% mercury chloride for 4 minutes, and then Wash 4 times with sterile water;

[0018] (2) Induction culture of callus: the detoxified explants were cut into 0.6cm length after absorbing the water on the surface, and then inserted into callus induction medium MS+6-BA 1.0mgL+2, 4-D 5mgL+IAA 0.5mgL+sucrose 35gL -1 + agar 6.0 g·L -1 The culture conditions were: pH value 6.0, 12 hours of light per day, light intensity of 1500 lx, culture temperature of 25°C, cultured for 15 days, and the callus induction rate was 100%;

[0019] (3) Suspension culture of callus: callus with vigorous growth, loose texture and no browning was cut out after 20 days of growth, and 30 g·L -1 The inoculum size was added to the liquid medium MS+6-BA1.0mgL added with sodium acetate -1 +NA...

Embodiment 2

[0023] (1) Treatment of explants: Select 1 to 2 tartary buckwheat stem segments near the terminal buds of tartary buckwheat plants that grow healthy and free from diseases and insect pests as explants, disinfect with 0.1% mercuric chloride for 6 minutes, and then use Wash with sterile water 3 times;

[0024] (2) Induction culture of callus: the detoxified explants were cut into 1.0 cm length after absorbing the water on the surface, and then inserted into callus induction medium MS+6-BA 0.5mgL+2, 4-D 2mgL + sucrose 50gL -1 + agar 6.0 g·L -1 The culture conditions were as follows: pH value 5.8, 10 hours of light per day, light intensity of 1200 lx, and culture temperature of 25°C;

[0025] (3) Suspension culture of callus: cut out the callus grown for 24 days, and add 40 g·L -1 The inoculum size was added to the liquid medium MS+6-BA1.5mgL added with sodium acetate -1 +NAA0.4mgL -1 + sucrose 30gL -1 +Vc 150mg L -1 Suspension culture was carried out in the medium, the cul...

Embodiment 3

[0029] Change the amount of sodium acetate added in step (3) of Example 1 to 10 mg / L, and other steps are the same as in Example 1. After 25 days of culture, the biomass multiplication factor of buckwheat cells is 2.15, which is higher than that before adding any The biomass multiplication factor 2.03 of the control group fed with body feed was 5.91% higher; while the total flavonoid content in the assay culture was 5.46%, which was 3.30% higher than the total flavonoid content of the control group without any precursor feed 65.45%. This shows that in the liquid medium of buckwheat cells, when the concentration of sodium acetate precursor feed increased to 10 mg / L, the biomass proliferation of buckwheat cells did not increase significantly, but the growth rate of buckwheat cells in buckwheat cells The content of total flavonoids has obvious promoting effect.

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Abstract

The invention discloses a tissue culturing method for effectively improving a general flavone content of tartary buckwheat. The tissue culturing method uses a precursor feeding technology. A metabolic intermediate sodium acetate or yeast extract of a flavonoid matter is added in a buckwheat cell suspension culture, so that not only can the general flavone content in the buckwheat culture cell be obviously improved, but also the tissue culturing method has obvious acceleration effect on biomass multiplication of a tissue culture matter. According to the tissue culturing method provided by the invention, the foundation is laid for further developing a buckwheat plant secondary metabolite in a large scale, so that the rapid generation of effective components of the buckwheat plant is achieved; and meanwhile, the tissue culturing method can provide important technical support for further research on molecule conditioning of flavones secondary metabolism in the buckwheat plant.

Description

technical field [0001] The invention relates to a buckwheat tissue culture method, in particular to a tissue culture method which can effectively increase the total flavonoid content of buckwheat. Background technique [0002] Buckwheat is a plant with the same source of medicine and food. There are two kinds of buckwheat, tartary buckwheat and sweet buckwheat. Because tartary buckwheat contains a lot of flavonoids in its seeds, roots, stems, leaves, flowers and other organs, it is known as a "three-lowering food for lowering blood sugar, blood pressure, and blood fat". With the continuous discovery of its nutritional value and medicinal value, tartary buckwheat plant resources are more and more favored by people. However, due to the characteristics of self-pollination of tartary buckwheat plants, it is difficult to succeed in artificial hybridization in agricultural production and breeding, which is also an important reason why it is difficult to obtain high-yield and exce...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王跃华赵钢宋超孙雁霞段有丽陈丽邬晓勇吴佳靓覃泽娇王朝君熊云翔
Owner CHENGDU UNIV
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