Kit for treating human bone marrow, umbilical cord blood, and peripheral blood cells, and cell treatment method

A processing method and umbilical cord blood technology are applied in the application field of biomedical technology, which can solve the problems of unknown impact, high cost, pain of patients, etc., and achieve the effects of avoiding the risk of microvascular embolism, easy use and preparation, and avoiding red blood cell interference.

Inactive Publication Date: 2013-03-13
WUHAN HAMILTON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a human bone marrow, umbilical cord blood, and peripheral blood cell processing kit and cell processing method with strong operability, high clinical safety, and easy clinical promotion. In the separation technology, there are problems such as high cost, low cell activity, unknown impact of markers on the human body after entering the human body, the need to inject mobilizing agents, causing pain to patients, cumbersome and not suitable for clinical use, etc.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0026] The present invention has developed a bone marrow, umbilical cord blood, peripheral blood cell processing kit, wherein the kit contains the following four reagents:

[0027] Solution A is the diluent: PBS solution;

[0028] Liquid No. B is a density liquid: sodium diatrizoate-polysucrose 400# or HISTOPAQUE1077 or Ficoll; sodium diatrizoate-polysucrose 400# is a density liquid prepared from polysucrose and sodium diatrizoate with a density of 1.075±0.001;

[0029] Liquid No. C is washing liquid: 0.9% sodium chloride injection;

[0030] Solution D is red blood cell removal solution: 0.8% ammonium chloride solution;

[0031] The present invention also discloses a cell treatment method using the above kit, the specific steps of which are: adding bone marrow or umbilical cord blood or peripheral blood containing sodium citrate anticoagulant to liquid A at a ratio greater than or equal to 1:1, Mix well; according to the volume of the diluted sample, take an appropriate numb...

Embodiment 2

[0034] The steps of the method for isolating umbilical cord blood stem cells using the kit of the present invention:

[0035] The composition of the kit is as follows:

[0036] Solution A is the diluent: PBS solution;

[0037] Liquid B is density liquid: Ficoll solution;

[0038] Liquid No. C is washing liquid: 0.9% sodium chloride injection solution;

[0039] Solution D is red blood cell removal solution: 0.8% ammonium chloride solution;

[0040] The above-mentioned No. A solution was sterilized by 10 pounds for 10 minutes, and its endogen content was detected to be less than or equal to 0.5 EU / ml, bottled, and stored at 4°C. Liquid B was sterilized by 10 pounds for 10 minutes, and its endotoxin content was detected to be ≤0.5EU / ml, and then bottled.

[0041] The steps of using the above kit to isolate 100ml of umbilical cord blood stem cells are as follows:

[0042] (1) Take 100ml of solution A, dilute the cord blood at a ratio of 1:1, mix well, and store it at 4 degrees ...

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PUM

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Abstract

The invention discloses a kit for treating human bone marrow, umbilical cord blood, and peripheral blood cells, and provides a kit for treating human bone marrow, umbilical cord blood, and peripheral blood cells and a cell treatment method which are strong in operationality, high in clinical safety, and convenient for clinical popularization. The kit comprises four reagents: No. A liquid is a diluent; No. B liquid is density fluid; No. C liquid is washing liquid; No. D liquid is erythrocyte-removing liquid. The invention fundamentally solves the problems of high cost, low cell activity, undefined human body influence for markers entering human body, pain for patients due to mobilization agent injection, cumbersome and inapplicable operations, and the like for current cell separation technology.

Description

technical field [0001] The present invention belongs to the application field of biomedical technology. Specifically relates to a method for separating stem cells in human bone marrow, umbilical cord blood and peripheral blood in vitro, in particular to a human bone marrow, umbilical cord blood, peripheral blood stem cell processing kit and a cell processing method. Background technique [0002] With the continuous development of science and technology, the separation technology for human blood cells has been continuously developed and optimized. The current stem cell separation methods include immunomagnetic bead method, flow cytometry method, blood cell separator method, culture expansion method And density gradient centrifugation, etc., but there are many disadvantages. (1) The immunomagnetic bead method combines the antibody coated on the magnetic bead particles with the corresponding antigen-positive cells on the cell surface, and then removes the non-adsorbed cells th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0789C12N5/074
Inventor 武栋成
Owner WUHAN HAMILTON BIOTECH
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