Online separation and purification method for butanol, acetone and ethanol in fermentation liquor through in-situ adsorption by using resins

A technology of fermented liquid and ethanol, which is applied in the biological field to achieve the effects of low fermentation efficiency, energy saving and strong adsorption capacity

Active Publication Date: 2014-05-21
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are no reports and related patents on the simultaneous separation and purification of butanol, acetone and ethanol using resin in situ adsorption method coupling fermentation device

Method used

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  • Online separation and purification method for butanol, acetone and ethanol in fermentation liquor through in-situ adsorption by using resins
  • Online separation and purification method for butanol, acetone and ethanol in fermentation liquor through in-situ adsorption by using resins
  • Online separation and purification method for butanol, acetone and ethanol in fermentation liquor through in-situ adsorption by using resins

Examples

Experimental program
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Embodiment 1

[0047] A method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by resin in-situ adsorption;

[0048] ① if figure 1 As shown, the seed medium was first passed nitrogen in the seed tank for 10 minutes to deoxygenate, then sterilized at 121°C for 30 minutes, cooled to room temperature, inserted into the acetone butanol ethanol production bacteria, and the production bacteria were cultivated to the most active logarithm of growth growth period. The cultivation time until the logarithmic growth phase is 12-18 hours, preferably 15 hours; the cultivation temperature is 30-40°C, preferably 37°C. After the seed culture is completed, it is ready to be inserted into the fermenter.

[0049] ② if figure 1 As shown, first sterilize the fermentation medium at 121°C for 30 minutes, then pass nitrogen gas for 2 hours to remove oxygen, cool to room temperature, turn on the pump I to transfer the seed liquid containing acetone butanol ethanol p...

Embodiment 2

[0054] A method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by resin in-situ adsorption;

[0055] The difference with embodiment 1 is:

[0056] When the pH of the fermentation broth is lower than 5.0, ammonia water is automatically added to adjust the pH to above 5.0. When the fermentation starts, the pump II is not started. When the fermentation progresses to the logarithmic growth phase of the producing bacteria, the pump II is started to circulate the fermentation liquid between the fermenter and the adsorption device. The fermentation method is feed-fed fermentation. When the glucose concentration in the fermentation liquid drops below 10g / L, glucose is added to continue to provide carbon source for the fermentation until the fermentation is terminated.

[0057] The experimental data are shown in Table 1 and Table 2.

[0058] Summary: After the fermentation, the concentrations of butanol and ABE total solvent were 3.3% (w...

Embodiment 3

[0060] A method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by resin in-situ adsorption;

[0061] The difference with embodiment 1 is:

[0062] The adsorption device is filled with Dowex Optipore SD-2 resin.

[0063] The experimental data are shown in Table 1 and Table 2.

[0064] Summary: After the fermentation, the concentrations of butanol and ABE total solvent were 1.4% (w / v) and 2.3% (w / v), respectively, which were increased by 27.3% and 27.8% compared with Comparative Example 1. The consumption of glucose was 6.8% (w / v), which was 23.6% higher than that of Comparative Example 1. The concentrations of butanol and ABE total solvent recovered after desorption were 7.9% (w / v) and 10.0% (w / v), which were 7.2 times and 5.6 times higher than those in Comparative Example 1, respectively. The production intensity of butanol and ABE total solvent is 0.26g / L / h and 0.43g / L / h, which are 18.2% and 19.4% higher than that of Comparati...

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Abstract

The invention discloses an online separation and purification method for butanol, acetone and ethanol in fermentation liquor through in-situ adsorption by using resins, which belongs to the field of biotechnologies, and comprises the following steps: (1) cultivating butanol, acetone and ethanol producing strains; (2) carrying out online in-situ adsorption on butanol, acetone and ethanol by using resins in the process of fermentation; and (3) after the adsorption is completed, carrying out desorption, condensation and recovery on the obtained products so as to obtain crude butanol, acetone and ethanol products. The method disclosed by the invention has the beneficial effects of high fermentation efficiency and low separation and purification costs.

Description

technical field [0001] The invention relates to a method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by using resin in-situ adsorption, and belongs to the field of biotechnology. Background technique [0002] Butanol is an important liquid energy source and chemical, which can be obtained through microbial fermentation, usually using acetone-butanol-ethanol (ABE) fermentation to produce butanol, and at the same time produce acetone and ethanol, as well as a small amount of organic acids. (See non-patent literature for details: Kumar, M, Gayen, K..Developments in biobutanol production: new insights.Applied Energy, 88:1999-2012, 2011). However, the butanol concentration at the end point in the fermentation broth is usually between 1.0 and 2.0 % (w / v), the concentration of acetone is 0.5-1.0% (w / v). In addition, the boiling point of butanol is 117.7°C, which is 100°C higher than the boiling point of water. Therefore, if the tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/16C12P7/28C12P7/06C07C29/76C07C31/12C07C31/08C07C45/79C07C49/08C12R1/19C12R1/145
CPCY02E50/17Y02E50/10
Inventor 薛闯杨尚天白凤武
Owner DALIAN UNIV OF TECH
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