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Method for utilizing zeolite in-situ adsorption to separate and purify butanol, acetone and ethanol in fermenting solution online

A fermentation liquid and ethanol technology, applied in the biological field, to achieve the effects of low fermentation efficiency, improved fermentation efficiency, and strong adsorption capacity

Inactive Publication Date: 2013-03-13
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are no reports and related patents on the simultaneous separation and purification of butanol, acetone and ethanol using zeolite molecular sieve in-situ adsorption method coupling fermentation device

Method used

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  • Method for utilizing zeolite in-situ adsorption to separate and purify butanol, acetone and ethanol in fermenting solution online
  • Method for utilizing zeolite in-situ adsorption to separate and purify butanol, acetone and ethanol in fermenting solution online
  • Method for utilizing zeolite in-situ adsorption to separate and purify butanol, acetone and ethanol in fermenting solution online

Examples

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Effect test

Embodiment 1

[0046] A method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by using zeolite in-situ adsorption;

[0047] ① if figure 1 As shown, the seed medium was first passed nitrogen in the seed tank for 10 minutes to deoxygenate, then sterilized at 121°C for 30 minutes, cooled to room temperature, inserted into the acetone butanol ethanol production bacteria, and the production bacteria were cultivated to the most active logarithm of growth growth period. The cultivation time until the logarithmic growth phase is 12-18 hours, preferably 15 hours; the cultivation temperature is 30-40°C, preferably 37°C. After the seed culture is completed, it is ready to be inserted into the fermenter.

[0048] ② if figure 1 As shown, first sterilize the fermentation medium at 121°C for 30 minutes, then pass nitrogen gas for 2 hours to deoxygenate, cool to room temperature, turn on the pump I, and put the seed liquid containing acetone, butanol and e...

Embodiment 2

[0053] A method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by using zeolite in-situ adsorption;

[0054] The difference with embodiment 1 is:

[0055] The mass ratio of zeolite molecular sieve CBV28014 to fermentation broth is 1:10.

[0056] The experimental data are shown in Table 1 and Table 2.

[0057] Summary: After the fermentation, the concentrations of butanol and ABE total solvent were 1.5% (w / v) and 2.4% (w / v), respectively, which were 36.4% and 33.3% higher than those in Comparative Example 1. Glucose consumption was 7.4% (w / v), an increase of 34.5% compared with Comparative Example 1. The concentrations of butanol and ABE total solvent recovered after desorption were 5.2% (w / v) and 6.8% (w / v), respectively, which were 4.7 times and 3.8 times higher than those of Comparative Example 1. The production intensity of butanol and ABE total solvents are 0.25g / L / h and 0.42g / L / h, which are 13.6% and 16.7% higher than that...

Embodiment 3

[0059] A method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by using zeolite in-situ adsorption;

[0060] The difference with embodiment 1 is:

[0061] The mass ratio of zeolite molecular sieve CBV28014 to fermentation broth is 1:5.

[0062] The experimental data are shown in Table 1 and Table 2.

[0063] Summary: After the fermentation, the concentrations of butanol and ABE total solvent were 1.7% (w / v) and 2.8% (w / v), respectively, which were 54.5% and 55.6% higher than those in Comparative Example 1. Glucose consumption was 8.5% (w / v), an increase of 54.5% compared with Comparative Example 1. The concentrations of butanol and ABE total solvent recovered after desorption were 6.9% (w / v) and 9.0% (w / v), which were 6.3 times and 5.0 times higher than those of Comparative Example 1, respectively. The production intensity of butanol and ABE total solvents are 0.27g / L / h and 0.46g / L / h, which are increased by 22.7% and 27.8% comp...

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Abstract

The invention provides a method for utilizing zeolite in-situ adsorption to separate and purify butanol, acetone and ethanol in a fermenting solution online, and belongs to the field of a biological technology. The method comprises the following steps of: (1) culturing acetone, butanol and ethanol production bacteria; (2) fermenting and utilizing zeolite to adsorb the butanol, the acetone and the ethanol in situ and online; and (3) after the adsorption, desorbing, condensing and recycling to obtain butanol, acetone and ethanol rough products. The method disclosed by the invention has the beneficial effects that the fermentation efficiency is high and the separation and purification cost is low.

Description

technical field [0001] The invention relates to a method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by using zeolite in-situ adsorption, and belongs to the field of biotechnology. Background technique [0002] Butanol is an important liquid energy source and chemical, which can be obtained through microbial fermentation, usually using acetone-butanol-ethanol (ABE) fermentation to produce butanol, and at the same time produce acetone and ethanol, as well as a small amount of organic acids. (See non-patent literature for details: Kumar, M, Gayen, K..Developments in biobutanol production: new insights.Applied Energy, 88:1999-2012, 2011). However, the butanol concentration at the end point in the fermentation broth is usually between 1.0 and 2.0 % (w / v), the concentration of acetone is 0.5-1.0% (w / v). In addition, the boiling point of butanol is 117.7°C, which is 100°C higher than the boiling point of water. Therefore, if the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/16C12P7/28C12P7/06C07C29/76C07C31/12C07C31/08C07C45/79C07C49/08C12R1/19C12R1/145
CPCY02E50/17Y02E50/10
Inventor 薛闯杨尚天白凤武
Owner DALIAN UNIV OF TECH
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