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Triple immune colloidal gold rapid detection card and its preparation and use method

A technology of colloidal gold and quick test card, which is applied in the field of bioengineering to achieve the effect of simple operation, good anti-interference effect and unified judgment standard

Inactive Publication Date: 2013-03-13
WUXI FUYANG BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no public report on rapid detection test strips for the simultaneous detection of clenbuterol hydrochloride, ractopamine, albuterol, etc.

Method used

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  • Triple immune colloidal gold rapid detection card and its preparation and use method

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Experimental program
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Embodiment Construction

[0021] Provide following embodiment in conjunction with content of the present invention:

[0022] (1) Production of "Clenbuterol" triple quick test card

[0023] There are two holes on the upper plastic board strip, the sampling hole and the observation hole. The size of the sampling hole is 3mm×7mm, and the size of the observation hole is 4mm×18mm. The sample pads pasted side by side under the plastic card strips, the gold standard pads immobilized with colloidal gold-labeled specific monoclonal antibodies, NC membranes and absorbent pads. 25ng-50ng of three kinds of monoclonal antibodies were immobilized on the gold standard pad, and 0.4μg-1.2μg of three kinds of antigens-BSA were fixed on the NC membrane. Then place it at 37°C for 30 min in vacuum.

[0024] (2) Effect evaluation of the actual standard product of "Clenbuterol" triple rapid test card

[0025] ① Add 50 μL blank sample solution (pH 7.4, 0.2mol / L PBS: 8g sodium chloride, 3.35g disodium hydrogen phosphate dod...

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Abstract

The invention relates to an immune colloidal gold label rapid detection card, which applies the principle of competitive inhibition immunochromatography. During detection, first a sample to be detected is added into a sample adding hole. If the sample contains to-be-detected materials, antigens in the sample combine with colloidal gold-labeled specific monoclonal antibodies located on a gold label pad during side movement, thus inhibiting combination of the gold-labeled antibodies with corresponding antigen-BSA conjugates on NC film detection lines, and the detection lines (T lines) have no color, redundant gold-labeled monoclonal antibodies combine with redundant enzyme-labeled antibodies, and a control line (C line) present a color; and otherwise, the detection lines (T lines) are wine red, and the control line (C line) present a color. The rapid detection card provided in the invention can be applied to rapid, on-site and sensitive detection of Clenbuterol, Ractopamine and Salbutamol illicit drug residues in urine samples, tissue samples and the like.

Description

technical field [0001] The invention relates to a quick test card in the technical field of bioengineering and a method for using the same, in particular to a triple immune colloidal gold quick test card for simultaneous detection of clenbuterol (clenbuterol hydrochloride, ractopamine, and salbutamol) and its use method. Background technique [0002] Clenbuterol hydrochloride (Clenbuterol), ractopamine (Ractopamine), salbutamol (Salbutamol) and other β-adrenoceptor agonists can promote the growth of livestock and poultry, and then used in livestock and poultry feed additives, because the livestock and poultry after eating In the process of metabolism, it can promote the synthesis of protein and the degradation of fat, and improve the lean meat rate of livestock and poultry. In the 1990s, my country mistakenly regarded it as a scientific research achievement and began to introduce and promote it as a feed additive, which was commonly known as "clenbuterol". The drug is easy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531
Inventor 武爱波范峰刘木林毛爱民杨文杰刘刚陶海强徐伟钟国良
Owner WUXI FUYANG BIOTECH
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