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Molecular marker for raphanus sativus L. downy mildew resistant gene close linkage

A technology of molecular markers and resistance genes, applied in the field of crop genetics and breeding, can solve the problems affecting the accuracy of plant resistance identification results, resistance selection efficiency, and time-consuming, so as to improve the selection efficiency of radish downy mildew resistance, improve the Efficiency, the effect of overcoming environmental impact

Inactive Publication Date: 2013-03-20
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Radish downy mildew is an obligate parasitic fungus, and the identification and evaluation of resistance to downy mildew of radish involves multiple steps such as collection of pathogens, live storage, inoculation, plant management, and disease investigation. Traditional inoculation identification is not only time-consuming, but also affected by the concentration of inoculated spores , Inoculation environmental conditions and other factors have seriously affected the accuracy of plant resistance identification results and resistance selection efficiency

Method used

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  • Molecular marker for raphanus sativus L. downy mildew resistant gene close linkage
  • Molecular marker for raphanus sativus L. downy mildew resistant gene close linkage
  • Molecular marker for raphanus sativus L. downy mildew resistant gene close linkage

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Embodiment 1

[0038] 1. Screen radish resistant materials and build a genetic research group for radish downy mildew resistance

[0039] F 1 , F 1 F 2 , while F 1 Backcrossing produces BC.

[0040] 2 Identification of individual downy mildew resistance

[0041] Rinse the mold layer and its attachments on the surface of the harvested radish downy mildew leaves with tap water, then rinse with distilled water, and place them in a dark condition at 20°C to keep moisture (RH=95%), put the diseased leaves in a petri dish During the process, the leaves were covered with moist absorbent paper or filter paper) for 24 hours, and the newly produced sporangia were gently peeled off with wet cotton balls in distilled water to make a suspension. Spore concentration, final dilution to 1×10 5 spores / mL, and finally add 0.1% Tween 20 of the calibration volume to prepare the inoculum. At the 4-leaf stage of the seedlings, use the spray method to inoculate the single leaf, the inoculation amount is 150...

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Abstract

The invention belongs to the field of crop genetic breeding, and discloses a molecular marker for raphanus sativus L. downy mildew resistant gene close linkage. A sequence-related amplified polymorphism (SRAP) molecular marker method is used for carrying out marker genotyping on high-resistance downy mildew raphanus sativus L. parents (NAU-dhp08), high-sensitive downy mildew raphanus sativus L. parents (half green from autumn fields) and a second filial generation (F2) group single plant which is prepared through resistance and sensitive parent hybridization, through the F2 single plant resistance phenotype and marker gene linkage analysis, a resistance marker Em9 / ga24370 of 370-base pair (bp) close linkage is identified, a distance from a resistance locus and a marker genetic distance is 2.3 centimeters. The molecular marker method provided by the invention can be used for the marker assisted selection of raphanus sativus L. downy mildew resistance breeding, quickly and accurately identifies the raphanus sativus L. downy mildew resistance, overcomes the limitations of heavy workload, long period, easily influenced results by environment and the like of the conventional resistance identification method, the high-resistance gene selection efficiency can be obviously improved, so that a breeding process of a new high raphanus sativus L. downy mildew-resistant variety can be accelerated.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to molecular markers closely linked to radish downy mildew resistance genes. Background technique [0002] Radish (Raphanus sativus L.), also known as radish, is an important cruciferous root vegetable crop. my country has rich radish germplasm resources and a long history of cultivation; radish is mainly eaten with enlarged fleshy taproots, which has high nutrition Its value and therapeutic effect are deeply loved by people (Edited by Wang Longzhi and He Qiwei, Chinese Radish, Beijing: Science and Technology Literature Publishing House, 2005). [0003] Downy mildew (downy mildew) is a very harmful disease on radish production. The pathogen of this disease is an obligate living parasite belonging to the flagellate subphylum Oomycetes downy mildew. The disease is found all over the world. distributed. In recent years, the disease of radish downy mildew has occurred seriously,...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68A01H1/04
Inventor 柳李旺徐良蒋秋玮武剑龚义勤高相敏
Owner NANJING AGRICULTURAL UNIVERSITY
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