Method for continuously producing bacterial cellulose

A technology of bacterial cellulose and bacterial cellulose film, applied in the field of continuous production of bacterial cellulose, can solve the problems of poor homogeneity of bacterial cellulose, shedding, and inability of the machine to rotate, so as to reduce the time of zero cellulose production and improve production. Efficiency, the effect of improving utilization

Inactive Publication Date: 2013-03-20
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dynamic culture method can make up for the lack of static culture in terms of yield, but the obtained bacterial cellulose is mostly flocculent, clustered, spherical, star-shaped, etc., and its direct use value is low.
In addition, the homogeneity of the bacterial cellulose obtained through the turntable fermenter is poor. As the thickness of the bacterial cellulose film increases in the later stage of cultivation, it will fall off the turntable, and the flocculent cellulose in the culture solution will be entangled in the on the rotating shaft so that the machine cannot rotate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Preparation of fermentation medium;

[0038] Components of the fermentation medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, and amount of water;

[0039] The pH of the fermentation broth is 4.0;

[0040] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0041] 2) Bacteria expansion;

[0042] Transfer, inoculate and expand the cultured fermentation broth; the degree of expansion: the number of strain cells is 2×10 7 within the range of a / ml;

[0043] 3) Pre-cultivation;

[0044] Transfer the expanded bacterial liquid to a pre-culture tank filled with fermentation culture liquid, the height of the liquid level shall not exceed 5cm, place it in a constant temp...

Embodiment 2

[0054] 1) Preparation of fermentation medium;

[0055] Components of the fermentation medium, in mass percent, in wt%: 5 glucose, fructose, sucrose or mannitol, 0.5 peptone, 0.5 yeast extract, 0.1 citric acid, 0.2 disodium hydrogen phosphate, 0.1 potassium dihydrogen phosphate, and The amount is water; the pH of the fermentation broth is 6.0;

[0056] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0057] 2) Bacteria expansion;

[0058] Transfer, inoculate and expand the cultured fermentation broth; the degree of expansion: the number of strain cells is 2×10 9 within the range of a / ml;

[0059] 3) Pre-cultivation;

[0060] Transfer the expanded bacterial solution to a pre-culture tank filled with fermentation medium, the liquid level shall not exceed 5cm, place it in a constant temperature culture environment, an...

Embodiment 3

[0070] 1) Preparation of fermentation medium;

[0071] Components of the fermentation medium, in mass percent, in wt%: 5 glucose, fructose, sucrose or mannitol, 0.5 peptone, 0.5 yeast extract, 0.1 citric acid, 0.2 disodium hydrogen phosphate, 0.1 potassium dihydrogen phosphate, and amount of water;

[0072] The pH of the fermentation broth is 5.0;

[0073] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0074] 2) Bacteria expansion;

[0075] Transfer, inoculate and expand the cultured fermentation broth; the degree of expansion: the number of strain cells is 2×10 7 within the range of a / ml;

[0076] 3) Pre-cultivation;

[0077] Transfer the expanded bacterial solution to the pre-culture tank containing the fermentation medium, the liquid level shall not exceed 5cm, place it in a constant temperature culture envi...

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Abstract

The invention relates to a method for continuously producing bacterial cellulose. The method comprises three steps for fermenting and culturing the bacterial cellulose, comprises the step of pre-culturing, the step of continuous culturing, and the step of post-processing; the operation in rapid growth stage and the operation in stable growth stage of bacterial cells in division are independently carried out; and the transition time node of each link is precisely controlled. According to the method, bacterial cellulose original membranes are divided into an upper active layer and a lower bacterial cellulose membrane layer by virtue of the biological nature of the bacterial cellulose in the forming under the stage of collecting the bacterial cellulose original membrane in the continuous culturing link; and the activate layer can be recycled, so that the utilization rate of the bacterial cells which are inoculated into the fermenting and culture liquid at one time can be improved, and the continuous production of at least seven weeks of the bacterial cellulose can be realized.

Description

technical field [0001] The invention relates to a method for continuously producing bacterial cellulose, in particular to a method for continuously collecting bacterial cellulose. Background technique [0002] There have been many reports on the research on cellulose biosynthesis, among which the cellulose obtained through the biosynthesis of Gram-negative, prokaryotic Acetobacter xylinum has the advantages of high yield and high purity, so it is selected as the base for bacterial cellulose fermentation. template strain. The process of cellulose biosynthesis by Acetobacter xylinum can be divided into two processes: intracellular small molecule polymerization to form β-1,4-glucose macromolecular chains and extracellular microfibrils to gradually and hierarchically self-assemble to form a cellulose network network structure . The unique biosynthesis process of bacterial cellulose gives it a layered internal structure: First, the gaps between the microfibrils (cross-sectional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/01C12R1/02C12R1/38C12R1/41
Inventor 王华平杨敬轩李喆陈仕艳
Owner DONGHUA UNIV
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