Heparin sodium balance extraction method

An extraction method, the technology of heparin sodium, which is applied in the field of extracting heparin sodium, can solve the problems of decreased resin adsorption capacity, affecting resin adsorption, oil component clogging, etc., and achieves the effects of increased yield, reduced pollution, and easy operation

Inactive Publication Date: 2013-03-27
谭科
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The study of traditional methods found that there is a problem in the method of extracting heparin sodium by resin adsorption. After the resin is put into the adsorption tank, not all the resin has been fully utilized. When the resin is stirred and adsorbed, the enzymatic solution forms a vortex in the tank. The density of the bottom resin is higher than that of the upper part, just because the heparin concentration at the bottom is low and the concentration at the top is high, and there are impurities precipi

Method used

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Examples

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Embodiment 1

[0014] A method for balanced extraction of heparin sodium, comprising the following steps:

[0015] A mixed enzymatic hydrolysis solution: using traditional enzymatic hydrolysis technology, using healthy pig small intestinal mucosa as raw material, adding water, protease, casing salt, sodium hydroxide, the ratios are (this patent is calculated in parts), take 100 parts of intestinal mucosa Add: add 500L water: 500g protease: 3000g enteric salt, put them into the reaction tank together, after stirring evenly, adjust the pH of the enzymolysis solution to 8.0-9.0 with 2mol concentration of sodium hydroxide.

[0016] B. Ultrasonic working process: When the temperature in the enzymolysis solution reaches 40 degrees, add 28KHz, 40KHz, and 68KHz ultrasonic generators respectively, set the working time to 20 minutes, 30 minutes, and 10 minutes, and the power to 5000W, 4000W, and 5000W. . After the ultrasonic wave is over, the temperature is raised to 55-60°C, and the enzyme is hydrol...

Embodiment 2

[0024] The steps of this embodiment are the same as those in Example 1, except that the flow velocity and pressure of the adsorption chromatography column have been changed. In Example 2, the pressure gauge shows 1.02MPa, and the flow rate gauge shows 5m. 3 / H.

Embodiment 3

[0026] The steps of this embodiment are the same as those in Example 1, except that the flow velocity and pressure of the adsorption chromatography column have been changed. In Example 3, the pressure gauge shows 1.025MPa, and the flow rate gauge shows 10m 3 / H.

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Abstract

The invention discloses a heparin sodium extraction method. The heparin sodium extraction method is characterized in that the method solves problems of insufficient intestinal mucosa enzymatic hydrolysis and bad adsorption effect of traditional enzymatic hydrolysis technologies. A non-uniform enzymatic hydrolysis phenomenon is generated in the traditional enzymatic hydrolysis technologies. The method enables full enzymatic hydrolysis to be carried out through introducing different supersonic waves in different enzymatic hydrolysis phases, so the content of heparin sodium in the obtained enzymatic hydrolysis liquid is improved, and the heparin sodium is fully released. The method enables an adsorption mother liquid to be purified through filtering in an adsorption phase, so the adsorption environment is clean, the adsorption of the resin is easy, and pollution and obstruction are less, thereby the production efficiency of the heparin sodium is improved. The heparin sodium yield is improved by 10-20% through the above two steps, so the method has a substantial economic benefit.

Description

technical field [0001] The method of the invention relates to a technology for extracting heparin sodium using pig small intestinal mucosa as a material. The method improves the existing technical level through ultrasonic bio-enzymolysis technology and chromatography technology, thereby increasing the output of heparin sodium. Background technique [0002] Cardiovascular and cerebrovascular diseases are the number one killer of human diseases. With the overnutrition brought about by the improvement of people's living standards, the deterioration of the global environment, the accelerated pace of life, and the aging population, the morbidity and mortality of global cardiovascular and cerebrovascular diseases are increasing year by year. Disease sufferers create miracles of life. At present, heparin is the most effective and clinically used anticoagulant drug in the world. It is mainly used in the treatment of cardiovascular and cerebrovascular diseases and hemodialysis. Amon...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 谭科
Owner 谭科
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