Heparin sodium balance extraction method

Abstract

The invention discloses a heparin sodium extraction method. The heparin sodium extraction method is characterized in that the method solves problems of insufficient intestinal mucosa enzymatic hydrolysis and bad adsorption effect of traditional enzymatic hydrolysis technologies. A non-uniform enzymatic hydrolysis phenomenon is generated in the traditional enzymatic hydrolysis technologies. The method enables full enzymatic hydrolysis to be carried out through introducing different supersonic waves in different enzymatic hydrolysis phases, so the content of heparin sodium in the obtained enzymatic hydrolysis liquid is improved, and the heparin sodium is fully released. The method enables an adsorption mother liquid to be purified through filtering in an adsorption phase, so the adsorption environment is clean, the adsorption of the resin is easy, and pollution and obstruction are less, thereby the production efficiency of the heparin sodium is improved. The heparin sodium yield is improved by 10-20% through the above two steps, so the method has a substantial economic benefit.

Description

technical field [0001] The method of the invention relates to a technology for extracting heparin sodium using pig small intestinal mucosa as a material. The method improves the existing technical level through ultrasonic bio-enzymolysis technology and chromatography technology, thereby increasing the output of heparin sodium. Background technique [0002] Cardiovascular and cerebrovascular diseases are the number one killer of human diseases. With the overnutrition brought about by the improvement of people's living standards, the deterioration of the global environment, the accelerated pace of life, and the aging population, the morbidity and mortality of global cardiovascular and cerebrovascular diseases are increasing year by year. Disease sufferers create miracles of life. At present, heparin is the most effective and clinically used anticoagulant drug in the world. It is mainly used in the treatment of cardiovascular and cerebrovascular diseases and hemodialysis. Amon...

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Problems solved by technology

[0004] The study of traditional methods found that there is a problem in the method of extracting heparin sodium by resin adsorption. After the resin is put into the adsorption tank, not all the resin has been fully utilized. When the resin is stirred and adsorbed, the enzymatic solution forms a vortex in the tank. The density of the bottom resin is higher than that of the upper part, just because the heparin concentration at the bottom is low and the concentration at the top is high, and there are impurities precipitated at the bottom. In addition, the upper part of the resin floating in the adsorption tank is easily blocked by the oil components in the enzymolysis solution, and the adsorption capacity of the blocked resin is greatly reduced. These problems have affected resin adsorption, and the present invention adopts chromatographic method to solve this difficult problem for above-mentioned problem, do not find the related report that adopts chromatographic method in the crude product production of heparin sodium at present