Cultural method for continuous thickening of bacterial cellulose
A bacterial cellulose and culture method technology, applied in the field of bacterial cellulose cultivation, can solve problems such as shedding, poor homogeneity of bacterial cellulose, and inability to rotate the machine, and achieve the effects of simple preparation process, reduced collection, and reduced production costs
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Embodiment 1
[0043] 1) Preparation of fermentation medium;
[0044] Components of the fermentation medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, and amount of water;
[0045] The pH of the fermentation broth is 4.0;
[0046] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;
[0047] 2) Bacteria expansion;
[0048] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 5 pieces / ml.
[0049] 3) Static cultivation;
[0050] Transfer the expanded bacterial liquid to a culture container filled with fermentation culture liquid, the height of the liquid level should not exceed 5cm, place it in a constant temperature cultur...
Embodiment 2
[0068] 1) Preparation of fermentation medium;
[0069] Components of the fermentation medium, in mass percent, in wt%: 5 glucose, fructose, sucrose or mannitol, 0.5 peptone, 0.5 yeast extract, 0.1 citric acid, 0.2 disodium hydrogen phosphate, 0.1 potassium dihydrogen phosphate, and amount of water;
[0070] The pH of the fermentation broth is 6.0;
[0071] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;
[0072] 2) Bacteria expansion;
[0073] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 pieces / ml.
[0074] 3) Static cultivation;
[0075] Transfer the expanded bacterial liquid to a culture container filled with fermentation culture liquid, the height of the liquid level should not exceed 5cm, place it in a constant temperature culture env...
Embodiment 3
[0093] 1) Preparation of fermentation medium;
[0094] Components of the fermentation medium, in mass percent, in wt%: 5 glucose, fructose, sucrose or mannitol, 0.5 peptone, 0.5 yeast extract, 0.1 citric acid, 0.2 disodium hydrogen phosphate, 0.1 potassium dihydrogen phosphate, and amount of water;
[0095] The pH of the fermentation broth is 5.0;
[0096] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;
[0097] 2) Bacteria expansion;
[0098] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 pieces / ml.
[0099] 3) Static cultivation;
[0100] Transfer the expanded bacterial liquid to a culture container filled with fermentation culture liquid, the height of the liquid level shall not exceed 5cm, place it in a constant temperature culture envi...
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