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Researching method of blueberry fruit chemical components by virtue of acid hydrolysis

A blueberry fruit, chemical technology, applied in scientific instruments, material separation, analytical materials, etc.

Inactive Publication Date: 2015-02-11
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gas chromatography-mass spectrometry (GC-MS) is another broad-spectrum chemical component research method, which has the characteristics of wide linear range, high sensitivity, low cost of use, and wide application. Component reporting

Method used

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  • Researching method of blueberry fruit chemical components by virtue of acid hydrolysis
  • Researching method of blueberry fruit chemical components by virtue of acid hydrolysis
  • Researching method of blueberry fruit chemical components by virtue of acid hydrolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Grind the fresh patriot blueberry fruit with a juicer, accurately weigh 5g of the puree, immerse the puree in 100mL of methanol solution containing 0.1% formic acid, extract at room temperature for 2 hours, centrifuge at 1500rpm for 10 minutes, and take the supernatant; Put 0.5 mL of the supernatant into a 5 mL ground glass centrifuge tube, add 0.5 mL of trifluoroacetic acid solution with a concentration of 4 moL / L, and incubate at 80°C for 2 hours; take out the centrifuge tube, cool to room temperature, and centrifuge at 1000 rpm for 4 minutes. Transfer the supernatant to a 100mL chicken heart bottle, distill under reduced pressure to obtain a translucent purple-red adherent, dissolve the adherent in 1mL of methanol, place it in a centrifuge tube, dry it with a nitrogen blower, and add 0.5mL of BSTFA , put the centrifuge tube in an oven, keep it warm at 80°C for 1 hour, and obtain the derivatization solution; use the GCMS instrument to test the derivatization solution, ...

Embodiment 2

[0038]Grind the fresh fruit of Patriot blueberry with a juicer, accurately weigh 5g of the puree, immerse the puree in 150mL of 30% methanol solution, extract at room temperature for 2 hours, centrifuge at 1500rpm for 10 minutes, and take the supernatant; accurately measure the supernatant 0.5mL, put in a 5mL ground glass centrifuge tube, add 0.5mL trifluoroacetic acid solution with a concentration of 4moL / L, incubate at 100°C for 1 hour; take out the centrifuge tube, cool to room temperature, centrifuge at 1000rpm for 4 minutes, and remove the supernatant Transfer to a 100mL chicken heart bottle, distill under reduced pressure to obtain a translucent purple-red adherent, dissolve the adherent with 1mL of methanol, place it in a centrifuge tube, dry it with a nitrogen blower, add 0.5mL of BSTFA, and centrifuge The tube was placed in an oven and kept at 100°C for 1 hour to obtain a derivatization solution; the derivatization solution was tested with a GCMS instrument to obtain a...

Embodiment 3

[0040] Grind the fresh fruit of Patriot Blueberry with a juicer, accurately weigh 5g of the puree, immerse the puree in 100mL of 50% acetone solution, extract at room temperature for 2 hours, centrifuge at 1500rpm for 10 minutes, and take the supernatant; accurately measure the supernatant 0.5mL, put in a 5mL ground glass centrifuge tube, add 0.5mL trifluoroacetic acid solution with a concentration of 4moL / L, incubate at 80°C for 2 hours; take out the centrifuge tube, cool to room temperature, centrifuge at 1000rpm for 4 minutes, and remove the supernatant Transfer to a 100mL chicken heart bottle, and distill under reduced pressure to obtain a translucent purple-red adherent substance. Dissolve the adherent substance in 1mL methanol and place it in a centrifuge tube, dry it with a nitrogen blower, add 1mL BSTFA, and place the centrifuge tube Put it in an oven and keep it warm at 100°C for 1 hour to obtain a derivatization solution; use a GCMS instrument to test the derivatizati...

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Abstract

The invention provides a researching method of blueberry fruit chemical components by virtue of acid hydrolysis, and belongs to the technology of blueberry chemical component researches. The method comprises the following steps of 1) preparing blueberry fruit chemical component extract; 2) hydrolyzing the extract; 3) deriving the hydrolysate; 4) testing the derived liquid by using a gas chromatograph-mass spectrometer; and 5) acquiring the information of blueberry fruit compound components. According to the method, the blueberry fruit extract is hydrolyzed, and the components of the extract are researched by using precolumn derivatization of a gas chromatograph-mass spectrometer, so that process parameters are provided to comprehensive and thorough research of the blueberry fruit chemical components, and guidance is provided to reasonable utilization of blueberry resources with rich biomass.

Description

technical field [0001] The present invention relates to a method for researching the chemical components of blueberries, in particular to a method for studying the components of blueberry chemical components extracts by using gas chromatography-mass spectrometry (GC-MS) pre-column derivatization method after hydrolysis method. Background technique [0002] Blueberry is one of the five healthy fruits recommended by the World Food and Agriculture Organization. It has strong antioxidant capacity; it can prevent high blood pressure, high blood fat and obesity, and is especially good for eye health. Blueberries are rich in polyphenols, flavonoids, and polysaccharides, especially anthocyanins, which can improve vision and are important factors in providing blueberries with health benefits. [0003] The research on the chemical components of blueberry fruit is the basis of rational utilization of blueberry resources. In recent years, the structural identification of chemical comp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 吕兆林张柏林侯智霞潘月雷美玲任璇郭弘璇林西
Owner BEIJING FORESTRY UNIVERSITY
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