Unlock instant, AI-driven research and patent intelligence for your innovation.

Use of comfrey total polysaccharides in the preparation of medicines for preventing and treating acute lung injury and acute respiratory distress syndrome

A technology for acute respiratory distress and acute lung injury, applied in the new medicinal use of total polysaccharide from comfrey from traditional Chinese medicine, total polysaccharide from comfrey, preparation of medicines for preventing and treating acute lung injury and acute respiratory distress syndrome, which can solve the problems that have not yet been seen in Xinjiang. The total polysaccharide of comfrey arborvitae prevents and cures ALI and antipyretic reports, and there are few researches on the pharmacological effects of comfrey arborvitae polysaccharide in vivo, which can improve hypoxia and pulmonary inflammation, reduce aggregation, and relieve model symptoms.

Active Publication Date: 2015-08-26
FUDAN UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Throughout the research at home and abroad, there are few studies on the pharmacological effects of Xinjiang comfrey polysaccharides in vivo, and there are no reports on the prevention and treatment of ALI and antipyretic by Xinjiang comfrey polysaccharides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of comfrey total polysaccharides in the preparation of medicines for preventing and treating acute lung injury and acute respiratory distress syndrome
  • Use of comfrey total polysaccharides in the preparation of medicines for preventing and treating acute lung injury and acute respiratory distress syndrome
  • Use of comfrey total polysaccharides in the preparation of medicines for preventing and treating acute lung injury and acute respiratory distress syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take 100g of Xinjiang comfrey (Arnebia euchroma (Royle) Johnst) root medicinal material, crush it, extract 3 times with 95% ethanol cold soaking, dry the medicinal residue in a ventilated place at room temperature, then extract 3 times with hot water, filter and combine The extract is concentrated, centrifuged, the supernatant is used to remove free proteins with trichloroacetic acid; the supernatant is centrifuged, the supernatant is dialyzed with water for 3 days, the dialysate is concentrated to a small volume and ethanol is added to the alcohol content to 80%, centrifuged, and the precipitate is freeze-dried. Total polysaccharides. The sugar content of Xinjiang comfrey total polysaccharides measured by sulfuric acid-phenol method is 75.31%-77.12%; the uronic acid content of Xinjiang comfrey total polysaccharides measured by m-hydroxybiphenyl method is 22.58%-22.35%; Coomassie bright The protein content of Xinjiang Lithospermum total polysaccharide measured by blue me...

Embodiment 2

[0037] 48 SD male rats were randomly divided into 6 groups (groups A, B, C, D, E, and F). Group A is a pseudo-operation group, group B is a model, and groups C, D, and E are the low-dose group (40mg / kg, ig), medium-dose group (80mg / kg, ig) and high-dose of Xinjiang Lithospermum Polysaccharide Group (120mg / kg, ig), F group is the positive control group (hydroprednisone, hydroprednisone, 70mg / kg, ip). 8 per group.

[0038] Except for the sham operation group, the animals in the other groups were intraperitoneally injected with 1g / kg urethane anesthesia and fixed in the supine position. The right common carotid artery was separated and intubated for blood pressure monitoring, blood draw and reperfusion. After the animal was placed for 20 minutes to stabilize, blood was drawn from the common carotid artery to a mean arterial pressure of 40±5 mmHg and maintained in the following 20 minutes. After 1 hour, all blood collected was slowly returned within 1 hour. It is administered after...

Embodiment 3

[0046] 36 male Wistar rats (weight 190±20g), the rats were adapted to the experimental environment for at least 3 days before the experiment, freely ingested water, and performed adaptive body temperature manipulation twice a day. Record the measured body temperature, exclude stress-induced hyperthermia, and exclude those with large body temperature fluctuations (with a difference of 0.3°C for two consecutive times) or those whose body temperature exceeds 38°C. The day before the experiment, the rats were placed in a special squirrel cage with free drinking and fasting for 10 hours. The basal body temperature must be measured before the start of the formal experiment. The body temperature of the experimental animal was measured 3 times with an interval of 15 minutes, and the average of the 3 body temperatures was taken as the basal body temperature.

[0047] The animals were randomly divided into 6 groups (groups A, B, C, D, E, and F). Group A is the normal control group, group...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of traditional Chinese medicines, relates a novel pharmaceutical use of arnebia euchroma total polysaccharides from traditional Chinese medicines and especially relates to a use of arnebia euchroma total polysaccharides in preparation of a drug for preventing and treating acute lung injuries and acute respiratory distress syndrome. The invention realizes separation and extraction of total polysaccharides extract from a traditional Chinese medicine Xinjiang arnebia euchroma. The arnebia euchroma total polysaccharides obtained by the invention has a yield more than 3%, polysaccharides content more than 75%, uronic acid content more than 25% and protein content less than or equal to 10%. An overall animal model test proves that the Xinjing arnebia euchroma total polysaccharides has an obvious protection effect on LPS-induced acute lung injuries of rats and an obvious alleviation effect on LPS-induced rat fever, can be prepared into a drug for preventing and treating acute lung injuries and acute respiratory distress syndrome, and can be further used for preparation of a drug for preventing and treating severe SARS, a drug for bringing down a fever, a drug for preventing and treating human avian influenza, and a drug for preventing and treating A(H1N1)-type influenza, or a drug for preventing and treating seasonal influenza.

Description

Technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a new medicinal use of the total polysaccharide of comfrey sourced from traditional Chinese medicine. In particular, it relates to the use of comfrey total polysaccharides in preparing medicines for preventing and treating acute lung injury and acute respiratory distress syndrome. Background technique [0002] Acute lung injury (ALI) refers to a combination of lung inflammation and increased lung permeability caused by diffuse alveolar capillary membrane damage after the body has been hit by severe infection, trauma, radiation, shock, etc. It is the early stage of acute respiratory distress syndrome (ARDS). The pathophysiological changes are dominated by decreased lung compliance, increased intrapulmonary shunt, and imbalance of ventilation / blood flow ratio, which is one of the more common clinical critical illnesses. The main manifestations are refractory hypoxemia, increa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K36/30A61P11/00
Inventor 陈道峰姜韵章蕴毅
Owner FUDAN UNIV