Method for extracting high-purity ursolic acid from paulownia leaves

A technology of paulownia leaves and ursolic acid, applied in the direction of steroids, organic chemistry, etc., can solve the problem that the purity of ursolic acid cannot meet the requirements of medicinal use, and achieve a simple and fast extraction method, good safety, and less impurities Effect

Inactive Publication Date: 2013-04-03
SHANDONG ACAD OF CHINESE MEDICINE +1
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Problems solved by technology

Patent CN1629180A discloses a method for preparing ursolic acid by an acid-alkali method. The raw material of this method does not involve wid...

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  • Method for extracting high-purity ursolic acid from paulownia leaves

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Embodiment 1

[0028] 1. Take the dried paulownia leaves and crush them. The content of fragments larger than 10mm×10mm is less than 5%. Take the crushed paulownia leaves and add 10 times the amount of 95% ethanol to reflux for extraction. Extract twice, each time for two hours , combine the extracts, recover ethanol until the solution has no alcohol smell, weigh the weight of the obtained extract, add 20 times the amount of distilled water, heat to boiling, make the extract form a suspension, and let stand at room temperature for 12 hours.

[0029] 2. Take the layered ursolic acid water precipitation solution, slowly pour out the upper layer of water and discard it, heat and dissolve the lower layer with 15 times the amount of ethanol, keep the solution temperature at 60°C, and adjust with 1mol / L sodium hydroxide to PH11.0, centrifuge or filter to remove the precipitate, adjust the supernatant to PH2.0 with 1mol / L hydrochloric acid, centrifuge or filter, discard the supernatant, dissolve the...

Embodiment 2

[0038] 1. Take 10kg of paulownia leaves that are simply crushed after drying, add 12 times the amount of 85% ethanol for reflux extraction, extract twice, each time for two hours, combine the extracts, recover the ethanol until the solution has no alcohol smell, and weigh the obtained extract Add 25 times the amount of distilled water, and heat to boiling to make the extract form a suspension, and let stand at room temperature for 12 hours.

[0039] 2. Take the layered ursolic acid water precipitation solution, slowly pour out the upper layer of water and discard it, heat and dissolve the lower layer with 12 times the amount of ethanol, keep the temperature of the solution at 40°C, and use 0.1mol / L sodium hydroxide Adjust to pH 9.0, centrifuge or filter to remove the precipitate, adjust the supernatant to pH 3.0 with 0.1mol / L hydrochloric acid, centrifuge or filter, discard the supernatant, and dissolve the lower precipitate in ethanol and adjust the pH with sodium hydroxide ,...

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Abstract

The invention discloses a method for extracting high-purity ursolic acid from paulownia leaves. The method comprises the following steps: smashing paulownia leaves, extracting the smashed paulownia leaves with alcohol to obtain an extract; adding water into the extract for boiling; taking the water precipitation at the lower layer, dissolving the water precipitation with alcohol; adjusting pH to 9.0 to 11.0 with aqueous solution of sodium hydroxide at the temperature of 40 to 70 DEG C; filtering to remove precipitation; adjusting the pH to 1.0 to 3.0 with hydrochloric acid; filtering to take the precipitation; taking the precipitation once more; dissolving the precipitation with ethanol, and decolorizing and crystallizing with activated carbon to obtain the ursolic acid. The extracting method is simple, convenient and quick; the used reagent is only limited to ethanol, sodium hydroxide, hydrochloric acid, activated carbon and other auxiliary materials approved by pharmacopoeia rather than organic solvent and macroporous resin; no endogenic impurities are introduced; the obtained ursolic acid is stable in crystal form, small in impurity, and 98%-99.5% in purity, meets the reporting requirement of a new medicament, and can be used as a raw material of injection preparation.

Description

technical field [0001] The invention relates to a method for extracting a medicinally active single compound from plants, in particular to a method for extracting high-purity ursolic acid crystals available for injection from Paulownia leaves. Background technique [0002] Ursolic acid is a triterpenoid compound present in natural plants, which has various biological effects such as sedative, anti-inflammatory, antibacterial, anti-diabetic, anti-ulcer, lowering blood sugar, etc. Ursolic acid also has obvious antioxidant function , and thus is widely used as a raw material for medicine and cosmetics. Ursolic acid is widely distributed in nature and exists in a variety of plants, such as privet, loquat, hawthorn, paulownia, etc. Compared with other plants (such as privet, loquat, etc.), the content of ursolic acid in Paulownia leaves is moderate, but Paulownia is widely distributed, and Paulownia is mainly used as a timber tree. The leaves are low in cost and easy to collect,...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 徐新刚生立嵩闫雪生李义召
Owner SHANDONG ACAD OF CHINESE MEDICINE
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