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Preparation method of chicken embryo hybrid peptide with high angiotensin-converting enzyme inhibitory activity

An angiotensin and inhibitory activity technology, applied in the field of extracting mixed peptides with high ACE inhibitory activity, can solve the problems of extracting ACE inhibitory peptides and the like, and achieve the effects of low cost, abundant source materials and outstanding product effects

Inactive Publication Date: 2013-04-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant technical report on the extraction of ACE inhibitory peptides from chicken embryos using a simple separation method.

Method used

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  • Preparation method of chicken embryo hybrid peptide with high angiotensin-converting enzyme inhibitory activity

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preparation example Construction

[0028] A preparation method for chicken embryo high angiotensin-converting enzyme inhibiting activity mixed peptide, comprising the following steps:

[0029] (1) hatching of chicken embryos; (2) crushing and freeze-drying; (3) redissolving in water; (4) centrifugation; (5) ultrafiltration; (6) freeze-drying.

[0030] The specific steps are:

[0031] (1) Select fertilized eggs that are not harmful to health and put them in the incubator for incubation. The incubation temperature is controlled at 37.8°C and the relative humidity is 60%. During the peak period of component growth, various organs are basically formed. At this time, the fertilized eggs are taken out, put into a 4°C freezer to end their life and stored for processing;

[0032] (2) Disinfect the surface of the eggs, remove the eggshell and take out the chicken embryos. After crushing the chicken embryos with a tissue homogenizer at 4°C, pour them into a tray and put them in a freeze dryer for freeze-drying. After th...

Embodiment 1

[0041] Preparation method of chicken embryo high angiotensin-converting enzyme inhibitory activity mixed peptide:

[0042] (1) Select fertilized eggs that are not harmful to health, and put them in the incubator for incubation. The incubation temperature is controlled at 37.8°C and the relative humidity is 60%. During the peak period, various organs are basically formed. At this time, the fertilized eggs are taken out, placed in a 4°C freezer to end their life and stored for processing;

[0043] (2) Disinfect the surface of the eggs, remove the egg shell and take out the chicken embryos, crush the chicken embryos with a tissue homogenizer at 4°C, pour them into a tray and put them in a freeze dryer for freeze-drying, and wait until the samples are completely freeze-dried Finally, crush it into powder, seal it and store it in a -30°C refrigerator for processing;

[0044](3) Add sterile water to the dried chicken embryo powder to reconstitute according to the ratio of 5g powder...

Embodiment 2

[0049] Preparation method of chicken embryo high angiotensin-converting enzyme inhibitory activity mixed peptide:

[0050] (1) Eggs that have been hatched to the 15th day of life are selected for processing;

[0051] (2) Disinfect the surface of the eggs, remove the egg shell and take out the chicken embryos, crush the chicken embryos with a tissue homogenizer at 4°C, pour them into a tray and put them in a freeze dryer for freeze-drying, and wait until the samples are completely freeze-dried Finally, crush it into powder, seal it and store it in a -30°C refrigerator for processing;

[0052] (3) Add sterile water to the dried chicken embryo powder to reconstitute according to the ratio of 5g powder / 100mL sterile water, and stir evenly to obtain chicken embryo slurry;

[0053] (4) Centrifuge the chicken embryo slurry at 4°C and 5000×g for 20 minutes;

[0054] (5) Collect the centrifuged supernatant, conduct ultrafiltration through an ultrafiltration membrane with a molecular ...

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Abstract

The invention relates to a method for extracting a hybrid peptide with the high angiotensin-converting enzyme (ACE) inhibitory activity from a chicken embryo. The preparation method comprises the technological means of collecting healthy chick embryos hatched to certain days, and taking out the hybrid peptide product with high ACE inhibitory activity through processing means of tissue homogenate, freeze drying, redissolving with sterile water, refrigerated centrifugation, passing of an ultrafiltration membrane, freeze drying and the like. The finished product can be used as a functional factor to be added in food or serve as a component of a novel natural high-efficiency antihypertensive medicine to be developed. The preparation method is simple in means and low in cost; the obtained product has the advantages of nature, health, high activity and the like, and a potential medicinal development value for the middle-aged and elderly people, sub-healthy people with higher blood pressure and people allergic to chemical antihypertensive medicines; the preparation method is a high value-added processing method of chick embryos; and the hybrid peptide belongs to the application of homology therory of medicine and food of the traditional Chinese medicine in the field of healthcare.

Description

technical field [0001] The invention relates to a method for extracting active peptides, in particular to a method for extracting mixed peptides with high ACE inhibitory activity from chicken embryos. Background technique [0002] At present, with the improvement of people's living standards, hypertension has gradually become a major factor threatening human health. For hypertensive patients, high blood pressure often causes damage to organs such as the heart, brain, and kidneys, which poses a serious threat to life. [0003] In the physiological activities of blood pressure regulation, angiotensin-converting enzyme (ACE: peptidyl dipeptide hydrolase, EC 3, 4, 15, 1) plays a key role. On the one hand, it converts inactive angiotensin I into angiotensin II, which has the ability to strengthen myocardial contractility, and on the other hand, it can also inactivate bradykinin, which has a vasodilation effect. The synergistic effect of these two aspects , causing an increase i...

Claims

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Application Information

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IPC IPC(8): C07K14/81C07K1/14A23L1/29A23L1/305A23L33/00
Inventor 徐学明段翔杨哪吴凤凤金征宇周星田耀旗王金鹏焦爱权赵建伟谢正军
Owner JIANGNAN UNIV
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