OGP-CTx fusion protein for treating osteoporosis and relieving pain and nucleic acid encoding same

A technology for fusion protein and osteoporosis, which is applied in the field of fusion protein and nucleic acid encoding the fusion protein, which can solve the inconvenience of patients and achieve the effect of less psychological burden, long treatment process and safe use

Inactive Publication Date: 2013-04-03
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] If the patient takes multiple drugs to treat osteoporosis and relieve pain, drug antagonistic reactions are likely to occur; to stagger the time of taking drugs, on the one hand, the half-life of the drug must be considered, and on the other hand, the effective concentration of the drug must also be considered, which will cause inconvenience to the patient
[0005] There is currently no drug that can effectively treat osteoporosis and relieve pain at the same time

Method used

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  • OGP-CTx fusion protein for treating osteoporosis and relieving pain and nucleic acid encoding same
  • OGP-CTx fusion protein for treating osteoporosis and relieving pain and nucleic acid encoding same
  • OGP-CTx fusion protein for treating osteoporosis and relieving pain and nucleic acid encoding same

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specific Embodiment approach 1

[0012] Specific Embodiment 1: The amino acid sequence of the OGP-CTx fusion protein used in the treatment of osteoporosis and pain relief in this embodiment is shown in SEQ ID NO:2.

[0013] The nucleic acid sequence of the OGP-CTx fusion protein used in the treatment of osteoporosis and pain relief in this embodiment is shown in SEQ ID NO: 1. The nucleic acid sequence was synthesized by a bioengineering company to make a plasmid vector pUC( OGP / CTx). Digest the pUC (OGP / CTx) vector with BamH I and EcoRI double enzymes (the enzyme digestion reaction system is shown in Table 1, the enzyme digestion reaction was reacted at 37°C for 10 hours, and then 1.5% agarose gel electrophoresis, the target fragment Purified and recovered with DNA GEL EXTRACTION KIT), and double-digested pGEX-6P-1 with BamHI and EcoRI (the enzyme digestion reaction system is shown in Table 2, the enzyme digestion reaction was reacted at 37°C for 10h, and then 0.6% agarose gel electrophoresis, and the target...

specific Embodiment approach 2

[0060] Specific embodiment 2: The nucleic acid sequence of the OGP-CTx fusion protein used in the treatment of osteoporosis and pain relief in this embodiment is shown in SEQ ID NO: 1.

[0061] The nucleic acid sequence of the OGP-CTx fusion protein used in the treatment of osteoporosis and pain relief in this embodiment was synthesized by a bioengineering company.

[0062] In this embodiment, a KpnI restriction site is inserted between OGP and CTx in the nucleic acid sequence of the OGP-CTx fusion protein for treating osteoporosis and pain relief, which can improve the stability of the encoded fusion protein and also change the fusion protein The secondary structure improves its biological performance.

[0063] In this embodiment, BamHI and EcoRI restriction sites are respectively added on both sides of the fusion protein gene, and the coding base sequence of the fusion gene is redesigned according to the characteristics of Escherichia coli preferred codons.

[0064]

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Abstract

The invention provides an OGP-CTx fusion protein for treating osteoporosis and relieving pain and a nucleic acid encoding the same, relating to a fusion protein and a nucleic acid encoding the same and overcoming the defect that drugs for effectively treating osteoporosis and relieving pain at the same time do not exist at present. The amino acid sequence of the OGP-CTx fusion protein for treating osteoporosis and relieving pain is shown in SEQ ID NO:2. The sequence of the nucleic acid of the fusion protein is shown in SEQ ID NO:1. The fusion protein can be used in the field of drugs.

Description

technical field [0001] The present invention relates to a fusion protein and nucleic acid encoding the fusion protein. Background technique [0002] The incidence of osteoporosis in the elderly is high. There are 200 million osteoporosis patients in the world, and there are more women than men. According to the standards of the World Health Organization (WHO), the results of the US National Health and Nutrition Survey (NHANES III, 1988~1994) showed that osteoporosis seriously affects the quality of life of the elderly. Among the people over 50 years old, 1 / 2 of women, One fifth of men will suffer from osteoporotic fractures in their lifetime. Once a patient experiences the first osteoporotic fracture, the risk of secondary fractures increases significantly. The elderly in China rank first in the world, and there are 90 million osteoporosis patients, accounting for 7.1% of the total population. With the aging of society, the incidence of osteoporosis is on the rise, and it ...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61P19/10A61P29/00
Inventor 余琼
Owner HEILONGJIANG UNIV
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