Purification method of cortex mori total flavonoids

A purification method and technology of total flavonoids, which can be applied to pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc., and can solve the problem of low total flavonoid content

Inactive Publication Date: 2013-04-10
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defect of low total flavonoids content in the existing Cortex Morus alba total flavonoids extract, and provide a method for purifying total flavonoids of Cortex Morus alba, after purification, the content of total flavonoids can reach 53.6%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1: Take the crude extract of total flavonoids from Alba Cortex Mori, dissolve it into a crude extract solution equivalent to 0.7g / l of the crude drug, adjust the pH to 5-6, and apply the AB-8 type at a sample flow rate of 2BV / h Macroporous resin chromatography column chromatography, sample volume ≤ 2BV, after the adsorption is completed, first elute with 10BV distilled water, then use 70% ethanol solution to elute at a flow rate of 1BV / h until no flavonoids are present, and collect the ethanol eluate , concentrated and dried to obtain high-purity total flavonoids of Morus alba, the content of which can reach 53.6%.

Embodiment 2

[0064] Example 2: Take the crude extract of total flavonoids from Alba Cortex Mori, dissolve it into a crude extract solution equivalent to 0.5g / l of the crude drug, adjust the pH to 5-6, and apply AB-8 at a sample flow rate of 1.5BV / h Type macroporous resin chromatography column chromatography, sample volume ≤ 2BV, after the adsorption is completed, first elute with 12BV distilled water, then elute with 50% ethanol solution at a flow rate of 1.5BV / h until no flavonoids are present, collect ethanol wash Deliquoring, concentrating, and drying to obtain high-purity total flavonoids of Cortex Mori. The total flavonoid content is above 53%.

Embodiment 3

[0065] Example 3: Take the crude extract of total flavonoids of Cortex Mori, dissolve it into a crude extract solution equivalent to 1 g / l of the crude drug, adjust the pH to 5-6, and apply the AB-8 type at a sample flow rate of 2.5BV / h Macroporous resin chromatography column chromatography, sample volume ≤ 2BV, after the adsorption is completed, first elute with 8BV distilled water, then use 95% ethanol solution to elute at a flow rate of 1.2BV / h until no flavonoids are present, collect ethanol for elution liquid, concentrated, and dried to obtain high-purity total flavonoids of Cortex Mori. The total flavonoid content is above 53%.

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PUM

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Abstract

The invention discloses a purification method of cortex mori total flavonoids, which comprises the following steps of extracting a total flavonoids crude extract of cortex mori, dissolving the total flavonoids crude extract of the cortex mori into crude extract solution which is equivalent to 0.5-1g / l of crude drug in whole, adjusting the pH value to 5-6; performing chromatography on a chromatographic column of a macroporous resin at a loading flow rate of1.5-2.5 BV / h, wherein the loading volume is less than or equal to 2 BV; after absorption, eluting the crude extract solution by distilled water with the volume of 8-12 BV; then eluting the crude extract solution by 50-95 percent of ethanol solution at the flow rate of 1-1.5 BV / h until flavone dose not exist; and colleting an ethanol eluent, and then concentrating and drying the ethanol eluent to obtain a high-purity cortex mori total flavonoids extract, wherein the content of the cortex mori total flavonoids in the extract is over 53 percent, so the situation meets the requirement of measures for the administration of drug registration on that the content of effective parts accounts for over 50 percent of the extract, and the purification method can be directly used for preparing drugs.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extracts, in particular to a method for purifying total flavonoids of Alba Cortex Mori. Background technique [0002] Morus alba (Cortex Mori) Morus ( Morus alba L1) The dry root bark of Chinese herbal medicine is sweet and cold in nature, and returns to the lung meridian, which has the effects of purging lungs, relieving asthma, diuresis and detumescence. Morus alba mainly contains a variety of flavonoids, including Moluxin L., Morusin, cyclomorin, and Kuwanone. Cyclomulene etc. Literature reports that its total flavonoids have diuretic, antihypertensive, hypoglycemic, analgesic, sedative, anti-cancer and anti-HIV effects. Cortex Morus alba is usually extracted with ethanol solution to obtain a crude extract of total flavonoids. The content of total flavonoids in this crude extract is relatively low and cannot meet the requirements for use. At present, polyamides are u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/605A61P3/10A61P7/10A61P25/20A61P29/02A61P31/18A61P35/00
Inventor 周英
Owner GUIZHOU UNIV
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