Method for producing transfer factor in scale

Abstract

The invention relates to a method for extracting transfer factors from animal spleens, and mainly aims at providing a method which is applicable to in-scale transfer factor production. The method comprises the following steps of firstly crushing spleen cells to prepare a homogenate, secondly eliminating cell residues in the homogenate, and subsequently extracting the transfer factors, wherein the step of eliminating the cell residues in the homogenate comprises adjusting the pH value to 7.2, adding polyaluminium chloride into the homogenate according to a mass volume ratio of 1%, uniformly stirring, acting for 30 minutes, centrifuging for not less than 30 seconds, repeating centrifuging for three times, adding 0.5% of, 0.4% of and 0.1% of polyaluminium chloride according to the mass volume ratio each time, all uniformly stirring after adding polyaluminium chloride, acting for 30 minutes, primarily filtering the centrifugate, and finely filtering the primary filtrate by using a filtering film with a filtering precision of 0.2 microns so as to obtain the transfer factors. According to the method, the polyaluminium chloride sediment is adopted to eliminate the spleen cell residues in a breakthrough, so that the production method is applicable to in-scale production of transfer factors which are high in effective content and good in biological activity.

Description

technical field [0001] The invention relates to a method for extracting transfer factor from animal spleen. Background technique [0002] Lawrence et al. first discovered in 1953 that the dialyzable substance of white blood cells has the function of living lymphocytes, and called the substance causing this effect "transfer factor". [0003] Transfer factor is a mixture of small molecular peptides, ribose, amino acids and unknown factors. It has more than 200 components and its molecular weight is less than 10,000 Daltons. It has absorption characteristics for ultraviolet light. The maximum absorption wavelength is E230-280nm, and the average E250nm, not heat resistant, can be inactivated at 56°C for 30 minutes, but its activity is not destroyed by trypsin, DNase and RNase, stable after freezing and thawing, and the activity will not disappear for several years when stored below -20°C. Transfer factor is widely used in human medicine and veterinary medicine, and it is mainly...

AI Technical Summary

Problems solved by technology

This method involves technical fields such as molecular biology, fermentation engineering, and purification engineering. The technology is difficult, the equipment requirements are high, and the knowledge level and operational ability of practitioners are very high. At present, this technology is still in the research stage, and few products are available.

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