Assessment method of restoration effect of phenanthrene-polluted soil

A technology of polluted soil and evaluation method, applied in the restoration of polluted soil and other directions, can solve the problems of competition impact and soil ecological risk, and achieve the effects of simple model, real-time verification accuracy, and low earthworm mortality.

Inactive Publication Date: 2013-04-24
XINJIANG UNIVERSITY
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Problems solved by technology

Microbial remediation often requires the introduction of exogenous microorganisms into the soil. The degradation effect of these introduced obligate degrading bacteria or genetically

Method used

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  • Assessment method of restoration effect of phenanthrene-polluted soil
  • Assessment method of restoration effect of phenanthrene-polluted soil
  • Assessment method of restoration effect of phenanthrene-polluted soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Establishment of an evaluation method for the remediation effect of phenanthrene-contaminated soil

[0035] On the basis of the existing technology, the model is used to predict the degradation time and degradation rate, and then the two living indicators of corn germination rate and earthworm mortality are selected to form a comprehensive and accurate evaluation method.

[0036] The evaluation method includes three indicators: model establishment, maize germination rate and earthworm mortality.

[0037] (1) Establish the growth kinetics model of the phenanthrene-contaminated soil-degrading bacteria: by establishing the objective function equation of the growth kinetic parameters of the phenanthrene-contaminated soil-degrading bacteria:

[0038] F ( S ) = K R 0 ln S 0 ...

Embodiment 2

[0049] Example 2: Establishment of growth kinetic equation model of phenanthrene-contaminated soil degrading bacteria

[0050] Describe the rate of cell growth in terms of cell concentration:

[0051] By assuming that the available bacterial composition is represented by a parameter, that is, the change of bacterial concentration X over time:

[0052] dX dt = μX - - - ( 1 )

[0053] The growth of phenanthrene-contaminated soil-degrading bacteria is as follows:

[0054] μ = μ m S K + S - - - ( 2 )

[0055] Substituting formula (2) into formula (1), we get

[0056] dX ...

Embodiment 3

[0085] Embodiment three: corn germination test

[0086] (1) Activation culture of bacterial strains: LB culture medium is used to activate and culture the strains of phenanthrene-contaminated soil-degrading bacteria to obtain bacterial liquid for future use.

[0087] (2) Fermentation culture of strains: insert the bacterial solution of step (1) into the culture medium of step (1) according to the inoculation amount of 5% by volume; culture at 20°C-40°C, initial pH 4.0-8.0, and shake for 24h- After 72h, after the logarithmic growth phase of the thalli, centrifugation was carried out to obtain the thallus;

[0088] (3) Inoculation and degradation of the soil sample: the bacteria in step (2) are mixed with the soil sample containing the polycyclic aromatic hydrocarbon phenanthrene in a ratio of 10-50:100 by weight;

[0089](4) Select plump and similar-sized corn seeds, add the soil in step (3), and bury 20 seeds evenly in each flowerpot on the 0th, 3rd, 6th, 9th, and 12th days r...

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Abstract

The invention discloses an assessment method of a restoration effect of phenanthrene-polluted soil. The assessment method is characterized in that a growth kinetics model of phenanthrene-polluted soil degrading bacteria is established by utilizing a Matlab software, and the established model is simple and can scientifically reflect the growth rule of microbes; and the degradation ratio of phenanthrene in the soil is forecasted in real time and corn sprouting rate and angleworm mortality rate are selected as monitoring indexes, so that the restoration effect of the degrading bacteria to the phenanthrene-polluted soil can be visually reflected, the accuracy of the model can be simultaneously verified in real time, and the monitoring indexes are two living body indexes capable of being detected at any time. The assessment method of the restoration effect of the phenanthrene-polluted soil is established, and has practicability and actual value.

Description

field of invention [0001] The invention relates to the technical field of application of soil remediation microorganisms, in particular, the invention relates to the technical field of an evaluation method for the remediation effect of phenanthrene-contaminated soil. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) refer to a class of compounds in which two or more aromatic rings are fused together, and are ubiquitous in the environment. It has strong carcinogenicity, mutagenicity and teratogenicity, and has a strong inhibitory effect on microbial growth, and polycyclic aromatic hydrocarbons are more toxic after being irradiated by ultraviolet light. As early as the early 1980s, the U.S. Environmental Protection Agency identified 16 unbranched polycyclic aromatic hydrocarbons as priority pollutants in the environment. my country has also included polycyclic aromatic hydrocarbons in the blacklist of pollutants for environmental priority monitoring. There...

Claims

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Application Information

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IPC IPC(8): B09C1/10
Inventor 晁群芳杨滨银陈志丹王旭辉李磊尹丹丹陈爱华
Owner XINJIANG UNIVERSITY
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