Method for extracting low molecular weight collagen from cattle bone

A low-molecular-weight collagen technology, applied in the field of extracting low-molecular-weight collagen, can solve the problems of fine particle size and gelatin preparation, and achieve the effect of simple method and increased production

Active Publication Date: 2013-04-24
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, the bone pellet factory that provides raw materials for gelatin manufacturers produces a large amount of bone foam during the producti...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) The bone grains of the bovine bone after degreasing the raw material are pulverized by a pulverizer and ground into bone powder with a particle size of 0.5 to 1000 μm; the bone powder is dispersed in water to form bone slurry, and the weight ratio of bone powder to water is 1:3;

[0034] (2) adding hydrochloric acid to the bone slurry obtained in step (1), so that the pH of the bone slurry is 2.5, and after reacting for 1 hour, centrifuge; the obtained solid aggregate is washed with water until the pH is 5.0;

[0035] (3) Disperse the solid aggregate obtained after washing in step (2) in water to obtain bone slurry, the weight ratio of solid aggregate to water is 1:3; according to the pepsin to be added, add glacial acetic acid solution to the bone slurry Adjust the pH of the bone mud to 2.5;

[0036] (4) Heating the pH-adjusted bone slurry obtained in step (3) to 50° C., adding pepsin with a weight of 5‰ of the bone slurry to carry out enzymatic hydrolysis reaction...

Embodiment 2

[0043] (1) The bone grains of pig bones after degreasing the raw material are pulverized by a pulverizer and ground into bone meal with a particle size of 0.5 to 1000 μm; the bone powder is dispersed in water to form bone slurry, and the weight ratio of bone powder to water is 1:5;

[0044] (2) adding sulfuric acid to the bone slurry obtained in step (1), so that the pH of the bone slurry is 4.0, and after reacting for 1 hour, centrifuge; the obtained solid aggregate is washed with water until the pH is 5.7;

[0045] (3) Disperse the solid aggregate obtained after washing in step (2) in water to obtain bone mud, the weight ratio of solid aggregate to water is 1:4; according to the papain to be added, add sodium hydroxide to the bone mud The aqueous solution adjusts the pH of the bone mud to 6.0;

[0046] (4) Heating the pH-adjusted bone slurry obtained in step (3) to 60° C., adding papain with a weight of 0.5‰ of the bone slurry to carry out enzymatic hydrolysis reaction for 2...

Embodiment 3

[0053] (1) The bone grains of sheep bones after degreasing the raw materials are pulverized by a pulverizer and ground into bone powder with a particle size of 0.5-1000 μm; the bone powder is dispersed in water to form bone slurry, and the weight ratio of bone powder to water is 1:3;

[0054] (2) adding hydrochloric acid to the bone slurry obtained in step (1), so that the pH of the bone slurry is 2.5, and after reacting for 5 hours, centrifuging; the obtained solid aggregate is washed with water until the pH is 5.0;

[0055] (3) Disperse the solid aggregate obtained after washing in step (2) in water to obtain bone slurry, the weight ratio of solid aggregate to water is 1:3; add sodium hydroxide to the bone slurry according to the bromelain to be added The aqueous solution adjusts the pH of the bone mud to 6.0;

[0056] (4) Heating the pH-adjusted bone slurry obtained in step (3) to 55° C., adding bromelain with a weight of 0.05‰ of the bone slurry for enzymolysis reaction fo...

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Abstract

The invention belongs to the collagen hydrolysis field, and in particular to the method for extracting low molecular weight collagen from cattle bone. According to the invention, the cattle bone after being degreased is crushed and grinded to bone meal with particle size of 0.5-1000mum; the bone meal is dispersed in water to form bone slurry, a series postprocessing technology is employed to remove enzymatic hydrolysis and enzymes inactivating is carried out to obtain solid residues and soluble inorganic salt impurity in a protein liquid, thereby the low molecular weight collagen with high purity can be obtained. According to the invention, the low molecular weight collagen product with average molecular weight of 1000-5000g/mol can be obtained, the ash content is lower than 1.5%, and the obtained low molecular weight collagen can be used in food, medicine, and cosmetic and health product.

Description

technical field [0001] The invention belongs to the field of hydrolyzed collagen, in particular to a method for extracting low-molecular-weight collagen from livestock (including pigs, cattle, horses, and sheep) bones, and the low-molecular-weight collagen can be used in food, medicine, cosmetics and health care products. Background technique [0002] Low-molecular-weight collagen, also known as hydrolyzed collagen, is a product of collagen degradation and denaturation, with a molecular weight of 500-20,000 g / mol. In recent years, with the improvement of people's living standards, a wide variety of protein powders on the market have increasingly entered people's daily lives. These protein powders are derived from plants and animals respectively. There is a certain gap between the type and relative quantity of plant protein and the requirements of the human body, such as the lack of immunoglobulin in plant protein, and the relative lack of lysine in cereals and so on. In ad...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 张兵郭燕川王颖史京京
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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