Multi-functional pretreatment device used for amino acid analysis

A technology for processing equipment and amino acids, applied in analytical materials, material separation, measurement devices, etc., can solve problems such as stability and accuracy need to be improved, affecting the life of chromatographic columns, affecting chromatographic peak shapes, etc., to achieve excellent repeatability and structure. Simple, easy-to-make effects

Active Publication Date: 2013-04-24
DALIAN ELITE ANALYTICAL INSTR
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Problems solved by technology

However, excessive derivatization reagents will affect the life of the chromatographic column, so try to remove excess derivatization reagents before injection
Currently the most commonly used method is n-hexane extraction, which is easy to operate, but the derivatization reaction between PITC and amino acid is carried out under alkaline conditions (pH=9.0), and the sample after derivatization is alkaline, and direct injection analysis will affect the chromatographic peaks type, so it is necessary to dilute the sample by 2-4 times, and the dilution process of the sample will lead to a decrease in the sensitivity of the method
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  • Multi-functional pretreatment device used for amino acid analysis

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Embodiment Construction

[0020] Specific embodiments of the present invention will be described below.

[0021] Such as figure 1 As shown, a multifunctional pretreatment device for amino acid analysis, it includes a transparent box 1, the box 1 is preferably made of high temperature resistant glass; the upper part of the box 1 is provided with an openable and closable upper cover 2 , in order to ensure the airtightness of the closed state of the box body 1, a gasket 3 is also provided between the upper cover 2 and the box body 1; a sample rack 4 is arranged inside the box body 1, according to the specifications of the sample bottles to be placed Different, the sample rack 4 can be a structure or style that matches the sample bottle; a heating tube 5 and a temperature measuring tube 6 are also arranged in the box body 1, and the heating tube 5 is controlled by a temperature control meter 7 arranged outside the box body 1. Control; the upper cover 2 is provided with a nitrogen blowpipe 8 with a flow ra...

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Abstract

The present invention discloses a multi-functional pretreatment device used for amino acid analysis, and a pretreatment method by using the pretreatment device. This amino acid pre-treatment device is simple in structure and is cleverly designed, and integrates a variety of pretreatment functions in one, basically has all pretreatment functions for the amino acid analysis, and is convenient and simple in operation. The pretreatment device is simple in manufacturing process and low in manufacturing cost, and has a variety of advantages as it was, so the pretreatment device is particularly suitable for popularization and application in the current art, and has a very broad market prospect. Compared with the conventional methods, the amino acid pretreatment method provided by the present invention has relative excellent repeatability, and high stability and accuracy, as well as relative good sensitivity.

Description

technical field [0001] The invention relates to a pretreatment device for amino acid analysis and a pretreatment method for amino acid analysis using the device. Background technique [0002] Amino acid is the basic unit of protein, and it is a necessary analysis and test item in the production and scientific research of biology, medicine, health, agriculture and animal husbandry, food, beverage and feed and other industries. [0003] At present, amino acid analysis methods mainly include chemical analysis, electrochemical methods, spectrophotometry, high performance liquid chromatography, capillary electrophoresis, gas chromatography, etc., among which high performance liquid chromatography (HPLC) is the most commonly used method. one. High performance liquid chromatography includes two modes of ion exchange and reversed phase. The instrument used for ion exchange is an amino acid analyzer, which is high in cost, strong in specificity, and low in popularity. The reversed...

Claims

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Application Information

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IPC IPC(8): G01N30/06
Inventor 于淑新唐涛董礼孚张彦彬孙元社李彤
Owner DALIAN ELITE ANALYTICAL INSTR
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