Multi-functional pretreatment device used for amino acid analysis

Abstract

The present invention discloses a multi-functional pretreatment device used for amino acid analysis, and a pretreatment method by using the pretreatment device. This amino acid pre-treatment device is simple in structure and is cleverly designed, and integrates a variety of pretreatment functions in one, basically has all pretreatment functions for the amino acid analysis, and is convenient and simple in operation. The pretreatment device is simple in manufacturing process and low in manufacturing cost, and has a variety of advantages as it was, so the pretreatment device is particularly suitable for popularization and application in the current art, and has a very broad market prospect. Compared with the conventional methods, the amino acid pretreatment method provided by the present invention has relative excellent repeatability, and high stability and accuracy, as well as relative good sensitivity.

Description

technical field [0001] The invention relates to a pretreatment device for amino acid analysis and a pretreatment method for amino acid analysis using the device. Background technique [0002] Amino acid is the basic unit of protein, and it is a necessary analysis and test item in the production and scientific research of biology, medicine, health, agriculture and animal husbandry, food, beverage and feed and other industries. [0003] At present, amino acid analysis methods mainly include chemical analysis, electrochemical methods, spectrophotometry, high performance liquid chromatography, capillary electrophoresis, gas chromatography, etc., among which high performance liquid chromatography (HPLC) is the most commonly used method. one. High performance liquid chromatography includes two modes of ion exchange and reversed phase. The instrument used for ion exchange is an amino acid analyzer, which is high in cost, strong in specificity, and low in popularity. The reversed...

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Problems solved by technology

However, excessive derivatization reagents will affect the life of the chromatographic column, so try to remove excess derivatization reagents before injection

Currently the most commonly used method is n-hexane extraction, which is easy to operate, but the derivatization reaction between PITC and amino acid is carried out under alkaline conditions (pH=9.0), and the sample after derivatization is alkaline, and direct injection analysis will affect the chromatographic peaks type, so it is necessary to dilute the sample by 2-4 times, and the dilution process of the sample will lead to a decrease in the sensitivity of the method

In the industry standard draft of "Determination of Amino Acids in Rice Wine", adding a certain volume of 10% acetic acid aqueous solution to adjust the pH value of the sample is used, but this method is unstable. When the acetic acid is left for too long and the concentration changes slightly, the sample pH value will be corresponding Changes will seriously affect the chromatographic peak shape, and peak bifurcation phenomenon will appear

[0007] To sum up, the pretreatment steps of amino acid analysis at this stage are relatively cumbersome and complicated, requiring more equipment for operation, and its stability and accuracy need to be improved.

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