Preparation method for extracting antibacterial peptides from wood frog bodies by using ultrasonic waves

An ultrasonic wave and antimicrobial peptide technology, which is applied in the field of preparation of antimicrobial peptides extracted from Rana body by ultrasonic waves, can solve the problems of unsatisfactory antibacterial effect of antimicrobial peptide filtrate, unsatisfactory industrial production, difficulty in peeling Rana skin, etc., and achieve saving Raw materials, reduce labor intensity, reduce the effect of production costs

Active Publication Date: 2014-03-05
TONGHUA KANGYUAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its shortcoming is that the raw material directly adopts forest frog skin, which is difficult to peel, and it is labor-intensive and time-consuming, resulting in serious loss and waste, and it cannot meet the needs of industrial production at the same time.
And its antibacterial effect of the antibacterial peptide filtrate obtained by this process is not ideal enough

Method used

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  • Preparation method for extracting antibacterial peptides from wood frog bodies by using ultrasonic waves
  • Preparation method for extracting antibacterial peptides from wood frog bodies by using ultrasonic waves
  • Preparation method for extracting antibacterial peptides from wood frog bodies by using ultrasonic waves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A preparation method for directly extracting antimicrobial peptides from wood frogs by ultrasonic waves:

[0052] 1. Take 50 g of the dry body of the degreased and de-egged wood frog, and carry out the dedusting and impurity removal treatment.

[0053] 2. Add 500 mL of 5% acetic acid 10 times that of the frog body and swell at 10°C for 0.2 hours.

[0054] 3. Put the swollen frog body and 5% acetic acid solution 10 times larger than the frog body together into an ultrasonic wave with a power of 100W and a frequency of 20kHz for 15 minutes. Under the same conditions as in 2 and 3, extract 1 more time.

[0055] 4. Combine the extracts, centrifuge at 3000r.p.m for 20min, discard the precipitate, collect the supernatant, and follow steps 3 and 4 for one more operation.

[0056] 5. Combine the supernatants, heat at 50°C for 5 minutes, then lower to 10°C and filter.

[0057] 6. Concentrate to 1 / 3 volume under negative pressure of 0.5 atmospheres at 35°C.

[0058] 7. Collec...

Embodiment 2

[0060] A preparation method for directly extracting antimicrobial peptides from wood frogs by ultrasonic waves:

[0061] 1. Take 50g of the dry body of the forest frog, and carry out dust and impurity removal treatment.

[0062] 2. Add 750 mL of 6% acetic acid 15 times that of the frog body and swell at 15°C for 0.3 hours.

[0063] 3. Put the swollen frog body into an ultrasonic wave with a power of 150W and a frequency of 30kHz for 10 minutes. Under the same conditions as in 2 and 3, extract 1 more time.

[0064] 4. Combine the extracts, centrifuge at 5000r.p.m for 15min, discard the precipitate, collect the supernatant, and repeat steps 3 and 4 twice.

[0065] 5. Combine the supernatants, heat at 55°C for 10 minutes, then cool down to 10°C and filter.

[0066] 6. Concentrate to 1 / 5 volume under negative pressure of 0.5 atmospheres at 45°C.

[0067] 7. Collect the concentrated solution and store it at 4°C.

Embodiment 3

[0069] A preparation method for directly extracting antimicrobial peptides from wood frogs by ultrasonic waves:

[0070] 1. Take 50g of the dry body of the forest frog, and carry out dust and impurity removal treatment.

[0071] 2. Add 1000 mL of 7% acetic acid 20 times that of the frog body and swell at 20°C for 0.5 hours.

[0072] 3. Put the swollen frog body into an ultrasonic wave with a power of 200W and a frequency of 40kHz for 15 minutes. Under the same conditions as in 2 and 3, extract 1 more time.

[0073] 4. Combine the extracts, centrifuge at 5000r.p.m for 10min, discard the precipitate, collect the supernatant, and repeat steps 3 and 4 once.

[0074] 5. Combine the supernatants, heat at 60°C for 15 minutes, then reduce to 10°C and filter.

[0075] 6. Concentrate to 1 / 6 volume under negative pressure of 0.5 atmospheres at 55°C.

[0076] 7. Collect the concentrated solution and store it at 4°C.

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Abstract

The invention relates to a preparation method for extracting antibacterial peptides from wood frog bodies by using ultrasonic waves. A process for extracting biological antibacterial peptides disclosed by the invention comprises the following steps of: performing earlier stage cleaning treatment on dry wood frog bodies from which fat and ova are removed and which are taken as raw materials; performing acidifying treatment; extracting by adopting ultrasonic waves; and performing steps of centrifugal precipitation, filtration, concentration, collection and the like to obtain a wood frog antibacterial peptide concentrated solution. A novel process for directly extracting antibacterial peptides from wood frog bodies disclosed by the invention has the advantages of easy operation, high production efficiency, high yield, remarkable broad-spectrum bacterium-resisting and sterilizing efficiency, and the like, and can be widely applied to the fields of drugs, foods, fresh keeping of fruits and vegetables, daily cosmetics, feed additives and the like.

Description

technical field [0001] The invention relates to an extraction method of Rana antibacterial peptide, in particular to a preparation method for extracting antibacterial peptide from Rana body by ultrasonic waves. Its products can be widely used in the fields of medicine, food, fruit and vegetable preservation, daily cosmetics and feed additives. Background technique [0002] In the prior art, antimicrobial peptides have broad-spectrum antibacterial activity and have a strong killing effect on bacteria, especially the killing effect on some drug-resistant pathogenic bacteria has attracted people's attention. In view of the many characteristics of antimicrobial peptides, such as natural broad-spectrum antibacterial, heat resistance, non-toxic and non-drug residue, and not easy to produce drug-resistant strains, antimicrobial peptides may become another important new class of antibacterial drugs after traditional antibiotics such as penicillin. Therefore, the development and app...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/14
Inventor 林万君陆军滕利荣孟庆繁逯家辉王娟
Owner TONGHUA KANGYUAN BIOLOGICAL TECH
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