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A method for hydrolyzing whey protein and preparing peptone with compound enzyme

A whey protein and whey protein hydrolysis technology, applied in the direction of fermentation, etc., can solve the problems of unreasonable amino acid composition, low protein content, and low nutritional value of peptone products.

Active Publication Date: 2014-10-22
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] It can be seen that the current process of peptone production mainly uses soybeans, plant seeds, bones, blood, internal organs, etc. as raw materials. Although the price is low, the protein content is low, and the amino acid composition is not very reasonable. The nutritional value is not high, and at the same time, the yield of peptone is not very high

Method used

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  • A method for hydrolyzing whey protein and preparing peptone with compound enzyme

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The whey protein is prepared into a solution of 8% by weight with water, and the solution is centrifugally defatted (5000r / min, 55°C, the processing capacity is 20t / h), and the defatted solution is incubated in a hot water bath at 80°C for 15 minutes , adjust the pH value of the defatted solution to 9.0 with 1mol / L aqueous sodium hydroxide solution; then, add the compound enzyme of alkaline protease and flavor protease, the total addition amount of compound enzyme is 5% of the weight of whey protein added, wherein the addition ratio of alkaline protease to flavor protease is 0.6:1; this solution is hydrolyzed at 55°C for 4.0 hours, and inactivated in a water bath at 85°C for 10 minutes , and then use the ultrafiltration membrane to filter the solution. The molecular weight and aperture of the ultrafiltration membrane is 200,000 Da. The working pressure of the ultrafiltration membrane is 0.40MPa, and the working pressure of the membrane is 0.05MPa. The temperature was 42...

Embodiment 2

[0048] Whey protein is prepared into a 2% by weight solution with water, and the solution is centrifugally degreased (5000r / min, 55°C, processing capacity is 20t / h), and the defatted solution is incubated in a hot water bath at 60°C for 10 minutes , adjust the pH value of the degreased solution to 8.0 with 1mol / L aqueous sodium hydroxide solution; It is 2% of the weight of the whey protein added when preparing the whey protein aqueous solution, wherein the addition ratio of alkaline protease and flavor protease is 0.4: 1; this solution is hydrolyzed under the condition of 50 ℃ for 3.0 hours, at 80 ℃ Under the condition of water bath inactivation for 10 minutes, then use the ultrafiltration membrane to filter the solution. The molecular weight and pore size of the ultrafiltration membrane is 50,000 Da, the working pressure of the ultrafiltration membrane is 0.30MPa, and the working pressure of the membrane is 0.20 MPa, the working temperature of the ultrafiltration membrane is ...

Embodiment 3

[0051] Whey protein is prepared into a 10% by weight solution with water, and the solution is centrifugally degreased (5000r / min, 55°C, processing capacity is 20t / h), and the defatted solution is kept in a hot water bath at 90°C for 20 minutes , adjust the pH value of the defatted solution to 10.0 with 1mol / L sodium hydroxide aqueous solution; It is 4% of the weight of the whey protein added when preparing whey protein aqueous solution, wherein the addition ratio of alkaline protease and flavor protease is 0.7: 1; Under the condition of water bath inactivation for 10 minutes, then use the ultrafiltration membrane to filter the solution. The molecular weight and aperture of the ultrafiltration membrane is 200,000 Da. The working pressure of the ultrafiltration membrane is 0.40MPa, and the working pressure of the membrane is 0.10 MPa, the working temperature of the ultrafiltration membrane is 50° C.; then the permeate is concentrated by a reverse osmosis membrane, and the concen...

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Abstract

The invention provides a method of using a compound enzyme for hydrolysis of whey protein. According to the method, the compound enzyme is used for hydrolysis of whey protein, and a peptone product is produced by using a hydrolysis product. The method comprises the following steps: preparing an aqueous whey protein solution with a certain concentration; carrying out centrifugal degreasing; adjusting the pH value and temperature of feed liquid after heating and insulation in a hot bath for a period of time; adding a certain amount of endoprotease and exoprotease for compound hydrolysis for a period of time; heating hydrolysate to a temperature of about 90 DEG C; carrying out insulation for about 10 min and inactivating enzyme activity; carrying out ultrafiltration and reverse osmosis condensation; and finally carrying out freeze-drying to prepare whey peptone powder. According to the invention, the degree of hydrolysis of the produced peptone product is more than 60%, the product can be dissolved in water easily, yield of peptone is more than 50%, and the peptone product has good quality, contains more than 90% of oligopeptide and can be easily digested and absorbed.

Description

technical field [0001] The invention relates to a method for producing peptone, in particular to a method for hydrolyzing whey protein with compound enzymes and preparing peptone by ultrafiltration technology. Background technique [0002] As one of the main components of culture medium, peptone is widely used in many fields such as food, medicine, microbial culture, and biological fermentation. A good peptone product should not only be inexpensive, but also high in nutritional value. At present, the main raw materials for the production of peptone are mostly soybeans and scraps from the production and processing of animal products, such as bones, blood, internal organs, etc. Due to the difference in raw materials and production processes, the intrinsic quality and nutritional value of peptone products are also different. . [0003] At present, the commonly used process methods for producing peptone are: acid hydrolysis, alkali hydrolysis and protease hydrolysis. Although...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 石丹王彩云云战友高海燕秦文林漆晓明
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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