A method for hydrolyzing whey protein and preparing peptone with compound enzyme

Abstract

The invention provides a method of using a compound enzyme for hydrolysis of whey protein. According to the method, the compound enzyme is used for hydrolysis of whey protein, and a peptone product is produced by using a hydrolysis product. The method comprises the following steps: preparing an aqueous whey protein solution with a certain concentration; carrying out centrifugal degreasing; adjusting the pH value and temperature of feed liquid after heating and insulation in a hot bath for a period of time; adding a certain amount of endoprotease and exoprotease for compound hydrolysis for a period of time; heating hydrolysate to a temperature of about 90 DEG C; carrying out insulation for about 10 min and inactivating enzyme activity; carrying out ultrafiltration and reverse osmosis condensation; and finally carrying out freeze-drying to prepare whey peptone powder. According to the invention, the degree of hydrolysis of the produced peptone product is more than 60%, the product can be dissolved in water easily, yield of peptone is more than 50%, and the peptone product has good quality, contains more than 90% of oligopeptide and can be easily digested and absorbed.

Description

technical field

[0001] The invention relates to a method for producing peptone, in particular to a method for hydrolyzing whey protein with compound enzymes and preparing peptone by ultrafiltration technology. Background technique

[0002] As one of the main components of culture medium, peptone is widely used in many fields such as food, medicine, microbial culture, and biological fermentation. A good peptone product should not only be inexpensive, but also high in nutritional value. At present, the main raw materials for the production of peptone are mostly soybeans and scraps from the production and processing of animal products, such as bones, blood, internal organs, etc. Due to the difference in raw materials and production processes, the intrinsic quality and nutritional value of peptone products are also different. .

[0003] At present, the commonly used process methods for producing peptone are: acid hydrolysis, alkali hydrolysis and protease hydrolysis. Although...

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