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Preparation method of flaky nano-hydroxyapatite

A nano-hydroxyapatite and flaky technology, applied in nanotechnology, chemical instruments and methods, phosphorus compounds, etc., can solve the problems of rare reports and achieve safe and non-toxic reagents, simple process, and biocompatibility Good results

Inactive Publication Date: 2013-05-15
SHANGHAI NAT ENG RES CENT FORNANOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using a certain concentration of aminodextran as a template to synthesize sheet-like HA based on the principle of biomineralization, there are few reports on this aspect

Method used

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  • Preparation method of flaky nano-hydroxyapatite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Dissolve 0.1g of chondroitin sulfate (CS) in ultrapure water and stir at 4°C to make a 1.25% (w / v) CS solution; get 420ml of 0.1mol / L CaCl2 solution and add it into the prepared mixed solution, mix 20 minutes with slow heating to 40°C. Then slowly add NaH2PO4 solution (252ml, 0.1mol / L) and adjust the pH value with 0.1mol / L NaOH. When the pH value is 7, a white suspension appears and lasts for 24 hours. Centrifuge the saturated solution with a centrifuge (5000rpm, 5min), remove the supernatant, wash repeatedly with deionized water to remove salt ions, and dry at low temperature to obtain flaky nano-hydroxyapatite.

Embodiment 2

[0021] Dissolve 0.1g of hyaluronic acid (HA) in ultrapure water and stir at 4°C to make a 1.25% (w / v) HA solution; take 420ml of the CaCl2 solution with a concentration of 0.1mol / L and add it to the prepared mixed solution, mix 20 minutes with slow heating to 40°C. Then slowly add NaH2PO4 solution (252ml, 0.1mol / L) and adjust the pH value with 0.1mol / L NaOH. When the pH value is 7, a white suspension appears and lasts for 24 hours. Centrifuge the saturated solution with a centrifuge (5000rpm, 5min), remove the supernatant, wash repeatedly with deionized water to remove salt ions, and dry at low temperature to obtain flaky nano-hydroxyapatite.

Embodiment 3

[0023] Dissolve 0.1g of heparan sulfate (HS) in ultrapure water and stir at 4°C to make a 1.25% (w / v) HS solution; take 420ml of the CaCl2 solution with a concentration of 0.1mol / L and add it to the prepared mixed solution, mix 20 minutes with slow heating to 40°C. Then slowly add NaH2PO4 solution (252ml, 0.1mol / L) and adjust the pH value with 0.1mol / L NaOH. When the pH value is 7, a white suspension appears and lasts for 24 hours. Centrifuge the saturated solution with a centrifuge (5000rpm, 5min), remove the supernatant, wash repeatedly with deionized water to remove salt ions, and dry at low temperature to obtain flaky nano-hydroxyapatite.

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Abstract

The invention relates to a preparation method of flaky nano-hydroxyapatite. The method is characterized by comprising the steps of: dissolving amino-dextran in ultrapure water under 4DEG C to prepare a polysaccharide solution; adding a calcium salt solution into the polysaccharide solution prepared in step (1), slowly heating the solution to 40DEG C, and conducting stirring for 20min; adding a phosphate solution into the mixed solution prepared in step (2) slowly according to a Ca / P ratio of 1.67, and simultaneously adjusting the pH value with sodium hydroxide till the pH value becomes 7 and a white suspension liquid comes into being, keeping the pH value of the current solution at 7.0 for 24h; subjecting the precipitate generated in step (3) to cleaning, centrifugation and drying, thus obtaining flaky nano-hydroxyapatite powder. The flaky nano-hydroxyapatite prepared by the method is applicable to dentin and enamel biomimetic materials. The whole preparation process is simple, the adopted agents are safe and non-toxic, and the biocompatibility is good.

Description

technical field [0001] The invention relates to a method for preparing hydroxyapatite, in particular to a method for preparing flaky nano-hydroxyapatite. The invention belongs to the technical field of material preparation. Background technique [0002] Nano-hydroxyapatite (hydroxyapatite, referred to as HA) is an important component of vertebrate bones and teeth. For example, the mass fraction of HA in human bone is about 65%, and the HA content in human teeth is more than 95%. In dentin and enamel, HA is a flaky crystal, and the extracellular matrix is ​​90% collagen and 10% non-collagen. The non-collagen mainly includes dentin-specific protein, aminodextran, a small amount of phospholipids and some growth factors, etc. [0003] Aminodextran is several kinds of heteropolysaccharides that are polymerized from disaccharide repeating units into a linear polymer chain, and its structure includes an uronic acid part (hexuronic acid) and an amino sugar part (N-acetylhexosamine...

Claims

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Application Information

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IPC IPC(8): C01B25/32B82Y40/00
Inventor 陈玉云徐宁许国华叶晓键钟健何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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