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Saccharomycete for hydrolyzing starch, producing lactic acid and fixing nitrogen, as well as application thereof

A technology of yeast and starch solution, which is applied in the field of microorganisms to achieve the effect of reducing costs and production costs

Inactive Publication Date: 2015-04-29
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The living microorganisms currently used in microbial fertilizers are all prokaryotes, such as rhizobia, nitrogen-fixing bacteria, nitrogen-fixing cyanobacteria, etc. Have not yet seen the report of eukaryote such as saccharomycetes nitrogen fixation, the saccharomycete provided by the present invention fills up this gap

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  • Saccharomycete for hydrolyzing starch, producing lactic acid and fixing nitrogen, as well as application thereof
  • Saccharomycete for hydrolyzing starch, producing lactic acid and fixing nitrogen, as well as application thereof
  • Saccharomycete for hydrolyzing starch, producing lactic acid and fixing nitrogen, as well as application thereof

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Embodiment Construction

[0036] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0037] The screening and identification process of the CCTCC M2011495 bacterial strain of the present invention will be further described in detail below, which is an explanation of the present invention rather than a limitation.

[0038] 1. The characteristics, usage and formula of the culture medium used

[0039] Basic inorganic salt: As a common component of the following related synthetic media, its composition is (mass ratio): K 2 HPO 4 0.2%; CaCl 2 0.02%; MgSO 4 .7H 2 O0.02%; NaCl0.005%, prepared from tap water.

[0040] 1) Bacterial source enrichment medium: natural medium, designed in imitation of my country's traditional pulp-water fermentation mode, for the enrichment of amylolytic yeast in nature; its composition (mass ratio): 2% common wheat flour, 1% salt %, prepared from ta...

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Abstract

The invention discloses saccharomycete for hydrolyzing starch, producing lactic acid and fixing nitrogen, as well as application thereof. The saccharomycete belongs to Candida tropicalis, and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC M 2011495. The saccharomycete can be used for hydrolyzing starch, secreting lactic acid and self-producing fixed nitrogen, becomes the first multifunctional novel saccharomycete strain in current report, can be applied to lactic acid fermentation and single-cell protein production, or serves as an active strain source in microbial nitrogenous fertilizer, and has an extremely wide application prospect in food industry, agriculture, pharmacy and other fields.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, relates to a new yeast, in particular to a nitrogen-fixing yeast producing lactic acid and amylolysis and application thereof. Background technique [0002] Yeasts are a group of unicellular eukaryotic microorganisms known for their ability to grow in sugar-rich environments, be facultatively aerobic, and produce alcohol and carbon dioxide in anoxic environments. Commonly used in wine fermentation and bread, steamed bread making. The yeast of the present invention can secrete lactic acid with starch as a carbon source under anaerobic conditions, can self-generate nitrogen fixation under aerobic conditions, and has wide application value. [0003] Lactic acid is a non-toxic and non-polluting natural compound with a wide range of uses. The production strains currently used for lactic acid fermentation are mainly lactic acid bacteria and Rhizopus oryzae. During lactic acid fermentation, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P7/56C05F11/08C12R1/74
Inventor 杨水云王玲吴耀彬林杰黄敬亮龙建纲刘健康
Owner XI AN JIAOTONG UNIV