Method for cell perfusion culture to produce recombinant protein by microcarrier technology

A recombinant protein and perfusion culture technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as excessive accumulation of metabolic waste, and achieve the effects of avoiding accumulation, high yield, and increased yield
CN103103237AActive Publication Date: 2013-05-15哈药集团股份有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
哈药集团股份有限公司
Publication Date
2013-05-15

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Abstract

The invention discloses a method for large-scale and high density animal cell perfusion culture to produce recombinant protein by a microcarrier technology. The method is characterized in that: a shaking bioreactor is employed, a microcarrier suspends in the reactor and is in an uninterrupted flow state, a continuous perfusion way is adopted for continuous supplement of nutrients and metabolic waste is discharged, and at the same time the recombinant protein is harvested.
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Description

technical field

[0001] The invention belongs to the field of biopharmaceutical technology. Specifically, it relates to a method for producing protein by using microcarrier technology to perfuse and culture animal cells. Background technique

[0002] As therapeutic drugs, recombinant protein drugs have increasingly highlighted their important medical and commercial value. In general, larger, more complex proteins are produced using mammalian cell expression systems. Therefore, the high-level production of recombinant protein drugs depends on the selection and optimization of the culture process of mammalian cells.

[0003] At present, there are many animal cell culture techniques, which can be summarized into three categories, namely adherent culture, suspension culture and immobilized culture.

[0004] Adherent culture means that cells are attached to a certain solid surface for monolayer culture. Since the cells are fixed on the surface, no complex cell shut-off device...

Claims

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