Molecular marker primer combination for polymerization of wide-compatibility fertility site segments of indica-japonica subspecies and application thereof

Abstract

The invention discloses a molecular marker primer combination for the polymerization of wide-compatibility fertility site segments of indica-japonica subspecies and an application thereof. The primer combination consists of molecular marker primers W5, W7 and W17 respectively corresponding to fertility sites S5-n, S7-n and S17-n, wherein a left end primer sequence of the W5 is SEQ ID NO. 1, and a right end primer sequence of the W5 is SEQ ID NO. 2; a left end primer sequence of the W7 is SEQ ID NO. 3, and a right end primer sequence of the W7 is SEQ ID NO. 4; and a left end primer sequence of the W17 is SEQ ID NO. 5, and a right end primer sequence of the W17 is SEQ ID NO. 6. The application of molecular marker primer combination in crossbreeding is suitable for the breeding of wide-compatibility fertility site segment polymerization systems of hybridized descendants of rice indica and japonica, and is capable of solving the problem of infertility of intersubspecific hybrids between the indica and the japonica and promoting the utilization of superiority of the intersubspecific hybrids between the indica and the japonica.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and provides a combination of molecular marker primers for the aggregation of indica and japonica subspecies wide-compatibility fertility site fragments and its application. Background technique [0002] In recent years, with the continuous increase of my country's population and limited arable land, increasing rice yield is of great significance to ensure national food security, and the utilization of indica and japonica heterosis is an important way to increase rice yield. But indica-japonica inter-subspecific hybrid F1 usually exhibits partial sterility, which limits the effective use of its strong heterosis in production. The discovery of wide-compatibility gene S5-n provides a way to overcome the fertility barrier between indica and japonica subspecies (Japan.J.Breed., 1984,34:304-312). At present, the wide-compatibility gene S5-n is widely used in the utilization of indica and japonica he...

AI Technical Summary

Problems solved by technology

With the expansion of the range of breeding parents, researchers found that although one of the parents of some hybrid combinations is a wide-compatibility variety carrying S5-n, the hybrid F1 is still semi-sterile (Japan J Breed1993,43:507 –516), therefore, the wide-compatibility gene S5-n alone cannot completely solve the problem of hybrid semi-sterility between indica and japonica subspecies

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