A process for the clean production of chitin from waste biomass of shrimp and crab by solid-state fermentation with probiotics
A micro-ecological bacterial agent, waste biomass technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of insufficient process economy, insufficient resource utilization, and high recovery costs, achieving significant comprehensive benefits and improving resource comprehensiveness. The effect of utilization efficiency and low production cost
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Embodiment 1
[0033] Add 30Kg of medium containing 3.0% industrial glucose to 100Kg of shrimp and crab waste, and mix well; take EM bacterial solution (commercially available) and dilute it 200 times and spray it on the matrix with an inoculation amount of 0.3%, and then seal it in a fermenter for solid state Fermentation, fermentation temperature 35 ℃, fermentation 6d. After the fermentation is finished, the fermented product is squeezed to recover the biomass, and 0.5 times the volume of water is added to the slag after the first squeeze to squeeze again, and the feed liquid is combined. Wheat bran and cornstarch were added to the feed liquid, stirred and mixed, the water content of the mixed components was 20%, and further dried to obtain a micro-ecological feed additive, with a water content of 5.0%. The slag was soaked in acid and alkali to remove impurities to produce chitin, and a total of 9.7Kg chitin samples were obtained.
Embodiment 2
[0035] Add 60Kg of medium containing 9.0% starch to 100Kg of shrimp and crab waste, mix well and then inoculate the purchased EM bacterial agent. The commercially available EM bacterial solution is diluted 100 times and then sprayed on the substrate. The inoculation amount is 0.8%, and the number of viable bacteria 8.9×10 6 pcs / g. Afterwards, the closed solid-state fermentation is carried out in the fermentation tank, and the natural temperature is fermented for 4 days. After the fermentation is finished, the fermented product is squeezed to recover the biomass, and an equal volume of water is added to the slag after the first squeeze to squeeze again, and the feed liquid is combined. Add cornstarch and soybean meal powder to the feed liquid, stir and mix, the water content of the mixed components is 27%, and further dry to obtain the micro-ecological feed additive, the water content is 7.0%. The slag was soaked in acid and alkali to remove impurities to produce chitin, and ...
Embodiment 3
[0037]Add 100Kg of medium containing 7.0% maltodextrin to 100Kg of shrimp and crab waste, mix well and then inoculate the purchased EM bacteria agent. The commercially available EM bacteria solution is diluted 400 times and then sprayed on the matrix. The inoculation amount is 1.5%. The number is 1.7×10 8 pcs / g. Afterwards, it is closed and solid-state fermented in a fermentation tank, fermented at natural temperature, and fermented for 3 days. After the fermentation is finished, the fermented product is squeezed to recover the biomass, and twice the volume of water is added to the slag after the first squeeze to squeeze again, and the feed liquid is combined. Wheat bran and soybean meal powder were added to the feed liquid, stirred and mixed, and the water content of the mixed components was 36%, and further dried to obtain a micro-ecological feed additive, with a water content of 10.0%. The slag was soaked in acid and alkali to remove impurities to produce chitin, and a to...
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