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Cryopreservation solution of horse sperms and freezing method thereof

A technology of cryopreservation solution and freezing method, which is applied in the field of horse sperm cryopreservation solution and its freezing, and can solve the problems of unstable freezing effect, unrelated preparation and method of horse sperm freezing, low recovery rate, etc.

Active Publication Date: 2014-07-16
新疆维吾尔自治区畜牧科学院畜牧科学研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging from the disclosed information, the cryopreservation solution and freezing method of horse semen are still immature. The specific performance is that the quality of frozen sperm is poor, the recovery rate is low, and the freezing effect is very unstable due to individual differences, so it is difficult to apply to production practice.
There are relevant patent disclosures such as: a semen diluent for sex-controlled sperm cryopreservation; a cryoprotectant for mouse sperm; a cryopreservation solution for Tibetan mastiff sperm; a sperm freezing method and its cryopreservation solution, According to the search results, the preparation and method of equine sperm freezing have not been involved

Method used

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  • Cryopreservation solution of horse sperms and freezing method thereof
  • Cryopreservation solution of horse sperms and freezing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] 1) Processing method of mare's milk

[0031] Mix double-distilled water made in the laboratory with edible Arowana refined first-grade rapeseed oil (Yihai (Changji) Grain and Oil Industry Co., Ltd.) at a ratio of 1:1, bathe in 70°C water for 3 minutes, shake vigorously for 5 minutes, stand at room temperature until the water and oil separate, remove water, repeat this 3 times, centrifuge at 4500 rpm for 5min, and collect the oil;

[0032] Mix the collected oil with fresh mare's milk at a ratio of 1:6, shake well, place in a 70°C water bath for 5 minutes, shake vigorously for 5 minutes, centrifuge at 4500 rpm for 20 minutes, recover the middle layer of milk into a 100ml glass bottle, seal it and put it in an autoclave Sterilize at 90°C for 30 minutes and set aside;

[0033] 2) Preparation of LDL

[0034]Take 15ml of fresh egg yolk, dilute the yolk 3 times with 40ml of isotonic (9.945g / L) sodium chloride solution, stir for 1h, centrifuge at 10000×g for 45min at 4°C, and...

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Abstract

The invention provides cryopreservation solution of horse sperms and a freezing method thereof. The freezing method comprises the steps of firstly, applying mare milk and yolk low-density lipoprotein after special treatment to the cryopreservation solution of horse sperms at the same time; adding the cryopreservation solution by sub-steps in the freezing process; and controlling cooling, lower liquid nitrogen fumigation height and shorter fumigation time. The quality of freezing the horse sperms is obviously improved by the technology; the artificial insemination efficiency of frozen semen of the horse is greatly improved.

Description

technical field [0001] The invention relates to the preparation of an animal sperm cryopreservation solution, and is especially suitable for the preparation of a horse sperm cryopreservation solution. The cryopreservation solution prepared by the method has a stable and reliable freezing effect, and significantly improves the freezing quality of the horse sperm. Background technique [0002] The cryopreservation technology of domestic animal semen can be traced back to 1776. The Italian Spallanzanei first discovered that low-temperature substances (ice and snow) in nature can affect the activity of human sperm. In 1886, Montegazza reported that human sperm were frozen at -15°C. After treatment, some still survived, and the idea of ​​using low-temperature preservation of animal semen to preserve fine varieties was first proposed. Based on this idea, many scientists have successively applied different methods to human, frog, rabbit, rat, guinea pig and cattle. A large number o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 毋状元郑新宝霍飞陈静波董红张国庭范洪先海里且木阿依努尔
Owner 新疆维吾尔自治区畜牧科学院畜牧科学研究所
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